Restriction map for pF1KB7983
 (Linear) (Six-base) MAPSORT of: KIBB7983.seq  Check: 2765  from: 1  to: 1266



KIBB7983.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     129    1266 
   Size:        129    1137
 
AccI GT'mk_AC

Cuts at:      0     884    1266 
   Size:        884     382
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595     637     770    1266 
   Size:        595      42     133     496

  Fragments arranged by size: 

                 595     496     133      42
 
AfeI AGC'GCT

Cuts at:      0     981    1266 
   Size:        981     285
 
AgeI A'CCGG_T

Cuts at:      0     912    1266 
   Size:        912     354
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     684     716    1266 
   Size:        684      32     550

  Fragments arranged by size: 

                 684     550      32
 
ApaI G_GGCC'C

Cuts at:      0     111    1266 
   Size:        111    1155
 
ApoI r'AATT_y

Cuts at:      0     131     164    1266 
   Size:        131      33    1102

  Fragments arranged by size: 

                1102     131      33
 
AvaI C'yCGr_G

Cuts at:      0     716    1164    1266 
   Size:        716     448     102

  Fragments arranged by size: 

                 716     448     102
 
BanI G'GyrC_C

Cuts at:      0     469     501    1159    1168    1266 
   Size:        469      32     658       9      98

  Fragments arranged by size: 

                 658     469      98      32       9
 
BanII G_rGCy'C

Cuts at:      0     111    1266 
   Size:        111    1155
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     831    1266 
   Size:        831     435
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     497    1266 
   Size:        497     769
 
BglII A'GATC_T

Cuts at:      0     741    1266 
   Size:        741     525
 
Bme1580I G_kGCm'C

Cuts at:      0     111     283     472    1162    1171    1266 
   Size:        111     172     189     690       9      95

  Fragments arranged by size: 

                 690     189     172     111      95       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     258    1030    1266 
   Size:        258     772     236

  Fragments arranged by size: 

                 772     258     236
 
BmtI G_CTAG'C

Cuts at:      0      97     395    1266 
   Size:         97     298     871

  Fragments arranged by size: 

                 871     298      97
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     207    1266 
   Size:        207    1059
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     694    1266 
   Size:        224     470     572

  Fragments arranged by size: 

                 572     470     224
 
BsaWI w'CCGG_w

Cuts at:      0     457     912    1266 
   Size:        457     455     354

  Fragments arranged by size: 

                 457     455     354
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     268     298     667     697    1266 
   Size:        268      30     369      30     569

  Fragments arranged by size: 

                 569     369     268      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1044    1266 
   Size:       1044     222
 
BseYI C'CCAG_C

Cuts at:      0     464     997    1042    1266 
   Size:        464     533      45     224

  Fragments arranged by size: 

                 533     464     224      45
 
BsiEI CG_ry'CG

Cuts at:      0     115    1266 
   Size:        115    1151
 
Bsp1286I G_dGCh'C

Cuts at:      0     111     283     472    1162    1171    1266 
   Size:        111     172     189     690       9      95

  Fragments arranged by size: 

                 690     189     172     111      95       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     129     164    1028    1266 
   Size:        129      35     864     238

  Fragments arranged by size: 

                 864     238     129      35
 
BsrFI r'CCGG_y

Cuts at:      0     912    1266 
   Size:        912     354
 
BstYI r'GATC_y

Cuts at:      0     741    1266 
   Size:        741     525
 
BtgI C'CryG_G

Cuts at:      0    1007    1266 
   Size:       1007     259
 
BtsI GCAGTG_nn'

Cuts at:      0      58    1266 
   Size:         58    1208
 
EaeI y'GGCC_r

Cuts at:      0      55     112    1252    1266 
   Size:         55      57    1140      14

  Fragments arranged by size: 

                1140      57      55      14
 
EagI C'GGCC_G

Cuts at:      0     112    1266 
   Size:        112    1154
 
EarI CTCTTCn'nnn_

Cuts at:      0     792    1266 
   Size:        792     474
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1113    1266 
   Size:       1113     153
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     595     637     694     770    1266 
   Size:        224     371      42      57      76     496

  Fragments arranged by size: 

                 496     371     224      76      57      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     669    1266 
   Size:        107     562     597

  Fragments arranged by size: 

                 597     562     107
 
EcoRI G'AATT_C

Cuts at:      0     131    1266 
   Size:        131    1135
 
HaeII r_GCGC'y

Cuts at:      0     983    1266 
   Size:        983     283
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     268     300    1266 
   Size:        268      32     966

  Fragments arranged by size: 

                 966     268      32
 
HincII GTy'rAC

Cuts at:      0      48    1266 
   Size:         48    1218
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     599    1266 
   Size:        170     429     667

  Fragments arranged by size: 

                 667     429     170
 
MscI TGG'CCA

Cuts at:      0    1254    1266 
   Size:       1254      12
 
MspA1I CmG'CkG

Cuts at:      0      17     104     615    1266 
   Size:         17      87     511     651

  Fragments arranged by size: 

                 651     511      87      17
 
NcoI C'CATG_G

Cuts at:      0    1007    1266 
   Size:       1007     259
 
NdeI CA'TA_TG

Cuts at:      0    1127    1266 
   Size:       1127     139
 
NheI G'CTAG_C

Cuts at:      0      93     391    1266 
   Size:         93     298     875

  Fragments arranged by size: 

                 875     298      93
 
NspI r_CATG'y

Cuts at:      0     426    1071    1266 
   Size:        426     645     195

  Fragments arranged by size: 

                 645     426     195
 
PasI CC'CwG_GG

Cuts at:      0     203    1266 
   Size:        203    1063
 
PfoI T'CCnGG_A

Cuts at:      0    1221    1266 
   Size:       1221      45
 
PspOMI G'GGCC_C

Cuts at:      0     107    1266 
   Size:        107    1159
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     633     665    1266 
   Size:        633      32     601

  Fragments arranged by size: 

                 633     601      32
 
PstI C_TGCA'G

Cuts at:      0     140    1266 
   Size:        140    1126
 
PvuII CAG'CTG

Cuts at:      0     615    1266 
   Size:        615     651
 
SfcI C'TryA_G

Cuts at:      0     136     975    1266 
   Size:        136     839     291

  Fragments arranged by size: 

                 839     291     136
 
SphI G_CATG'C

Cuts at:      0    1071    1266 
   Size:       1071     195
 
StyI C'CwwG_G

Cuts at:      0    1007    1266 
   Size:       1007     259
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1005    1266 
   Size:       1005     261
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AgeI     AloI     ApaI     ApoI
     AvaI     BanI    BanII     BbsI    BciVI    BglII Bme1580I     BmrI
     BmtI     BplI     BpmI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
 Bsp1286I    BspMI    BsrFI    BstYI     BtgI     BtsI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I   HincII
     MmeI     MscI   MspA1I     NcoI     NdeI     NheI     NspI     PasI
     PfoI   PspOMI     PsrI     PstI    PvuII     SfcI     SphI     StyI
     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AhdI     AleI     AlfI
    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI    BmgBI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI     BsgI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI     NaeI     NarI   NgoMIV     NotI
     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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