Restriction map for pF1KE2390
 (Linear) (Six-base) MAPSORT of: KIEE2390.seq  Check: 1898  from: 1  to: 1503



KIEE2390.orf

 With 182 enzymes: SgfI * 

                        January 17, 2012 13:59  ..

AccI GT'mk_AC

Cuts at:      0      84    1503 
   Size:         84    1419
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1387    1503 
   Size:       1387     116
 
AleI CACnn'nnGTG

Cuts at:      0     226     499    1503 
   Size:        226     273    1004

  Fragments arranged by size: 

                1004     273     226
 
AlwNI CAG_nnn'CTG

Cuts at:      0     449    1503 
   Size:        449    1054
 
ApoI r'AATT_y

Cuts at:      0     363    1503 
   Size:        363    1140
 
AvaI C'yCGr_G

Cuts at:      0     429    1093    1503 
   Size:        429     664     410

  Fragments arranged by size: 

                 664     429     410
 
AvrII C'CTAG_G

Cuts at:      0     670    1503 
   Size:        670     833
 
BanI G'GyrC_C

Cuts at:      0      42    1503 
   Size:         42    1461
 
BanII G_rGCy'C

Cuts at:      0      15     836    1118    1503 
   Size:         15     821     282     385

  Fragments arranged by size: 

                 821     385     282      15
 
BbeI G_GCGC'C

Cuts at:      0      46    1503 
   Size:         46    1457
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     789    1503 
   Size:        789     714
 
BfrBI ATG'CAT

Cuts at:      0     767    1503 
   Size:        767     736
 
BglI GCCn_nnn'nGGC

Cuts at:      0     645    1503 
   Size:        645     858
 
BglII A'GATC_T

Cuts at:      0    1043    1503 
   Size:       1043     460
 
BlpI GC'TnA_GC

Cuts at:      0     262    1503 
   Size:        262    1241
 
BmgBI CAC'GTC

Cuts at:      0     336     372    1503 
   Size:        336      36    1131

  Fragments arranged by size: 

                1131     336      36
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1326    1503 
   Size:       1326     177
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     644    1118    1472    1503 
   Size:        262     382     474     354      31

  Fragments arranged by size: 

                 474     382     354     262      31
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     140     175     892    1094    1503 
   Size:        140      35     717     202     409

  Fragments arranged by size: 

                 717     409     202     140      35
 
BsaAI yAC'GTr

Cuts at:      0    1472    1503 
   Size:       1472      31
 
BsaHI Gr'CG_yC

Cuts at:      0      43      59    1466    1503 
   Size:         43      16    1407      37

  Fragments arranged by size: 

                1407      43      37      16
 
BsaWI w'CCGG_w

Cuts at:      0    1175    1290    1503 
   Size:       1175     115     213

  Fragments arranged by size: 

                1175     213     115
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     137     167    1503 
   Size:        137      30    1336

  Fragments arranged by size: 

                1336     137      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1302    1503 
   Size:       1302     201
 
BseYI C'CCAG_C

Cuts at:      0      27      36     266    1145    1503 
   Size:         27       9     230     879     358

  Fragments arranged by size: 

                 879     358     230      27       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     979    1503 
   Size:        979     524
 
BsiHKAI G_wGCw'C

Cuts at:      0     223     284    1503 
   Size:        223      61    1219

  Fragments arranged by size: 

                1219     223      61
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1314    1503 
   Size:       1314     189
 
Bsp1286I G_dGCh'C

Cuts at:      0      15     223     284     836    1118    1503 
   Size:         15     208      61     552     282     385

  Fragments arranged by size: 

                 552     385     282     208      61      15
 
BspEI T'CCGG_A

Cuts at:      0    1175    1290    1503 
   Size:       1175     115     213

  Fragments arranged by size: 

                1175     213     115
 
BspHI T'CATG_A

Cuts at:      0     591    1503 
   Size:        591     912
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     812    1001    1079    1503 
   Size:        812     189      78     424

  Fragments arranged by size: 

                 812     424     189      78
 
BsrBI CCG'CTC

Cuts at:      0      48    1004    1503 
   Size:         48     956     499

  Fragments arranged by size: 

                 956     499      48
 
BsrDI GCAATG_nn'

Cuts at:      0     755    1503 
   Size:        755     748
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     449    1503 
   Size:        449    1054
 
BstEII G'GTnAC_C

Cuts at:      0     462    1503 
   Size:        462    1041
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1398    1503 
   Size:       1398     105
 
BstYI r'GATC_y

Cuts at:      0    1043    1503 
   Size:       1043     460
 
BtgI C'CryG_G

Cuts at:      0      63     944    1391    1503 
   Size:         63     881     447     112

  Fragments arranged by size: 

                 881     447     112      63
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1311    1503 
   Size:       1311     192
 
ClaI AT'CG_AT

Cuts at:      0     293    1259    1503 
   Size:        293     966     244

  Fragments arranged by size: 

                 966     293     244
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     388    1503 
   Size:        388    1115
 
EaeI y'GGCC_r

Cuts at:      0     127     615     785    1388    1503 
   Size:        127     488     170     603     115

  Fragments arranged by size: 

                 603     488     170     127     115
 
EarI CTCTTCn'nnn_

Cuts at:      0     206     742     785    1204    1414    1503 
   Size:        206     536      43     419     210      89

  Fragments arranged by size: 

                 536     419     210     206      89      43
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      91     328     712    1503 
   Size:         91     237     384     791

  Fragments arranged by size: 

                 791     384     237      91
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     644    1118    1387    1472    1503 
   Size:        262     382     474     269      85      31

  Fragments arranged by size: 

                 474     382     269     262      85      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     674    1503 
   Size:        674     829
 
EcoO109I rG'GnC_Cy

Cuts at:      0     270     556    1445    1503 
   Size:        270     286     889      58

  Fragments arranged by size: 

                 889     286     270      58
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     249     753    1503 
   Size:        249     504     750

  Fragments arranged by size: 

                 750     504     249
 
HaeII r_GCGC'y

Cuts at:      0      37      46     696    1503 
   Size:         37       9     650     807

  Fragments arranged by size: 

                 807     650      37       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     137     169    1503 
   Size:        137      32    1334

  Fragments arranged by size: 

                1334     137      32
 
HincII GTy'rAC

Cuts at:      0      85     750    1503 
   Size:         85     665     753

  Fragments arranged by size: 

                 753     665      85
 
KasI G'GCGC_C

Cuts at:      0      42    1503 
   Size:         42    1461
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     428    1503 
   Size:        428    1075
 
MscI TGG'CCA

Cuts at:      0     617     787    1390    1503 
   Size:        617     170     603     113

  Fragments arranged by size: 

                 617     603     170     113
 
MslI CAynn'nnrTG

Cuts at:      0     226     499     590    1396    1503 
   Size:        226     273      91     806     107

  Fragments arranged by size: 

                 806     273     226     107      91
 
MspA1I CmG'CkG

Cuts at:      0      65     449    1065    1338    1503 
   Size:         65     384     616     273     165

  Fragments arranged by size: 

                 616     384     273     165      65
 
NarI GG'CG_CC

Cuts at:      0      43    1503 
   Size:         43    1460
 
NcoI C'CATG_G

Cuts at:      0     944    1391    1503 
   Size:        944     447     112

  Fragments arranged by size: 

                 944     447     112
 
NsiI A_TGCA'T

Cuts at:      0     769    1503 
   Size:        769     734
 
PasI CC'CwG_GG

Cuts at:      0       7    1503 
   Size:          7    1496
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1141    1503 
   Size:       1141     362
 
PfoI T'CCnGG_A

Cuts at:      0      89    1134    1460    1503 
   Size:         89    1045     326      43

  Fragments arranged by size: 

                1045     326      89      43
 
PpuMI rG'GwC_Cy

Cuts at:      0     270     556    1445    1503 
   Size:        270     286     889      58

  Fragments arranged by size: 

                 889     286     270      58
 
PshAI GACnn'nnGTC

Cuts at:      0    1450    1503 
   Size:       1450      53
 
PstI C_TGCA'G

Cuts at:      0     454     545    1343    1503 
   Size:        454      91     798     160

  Fragments arranged by size: 

                 798     454     160      91
 
PvuII CAG'CTG

Cuts at:      0     449    1338    1503 
   Size:        449     889     165

  Fragments arranged by size: 

                 889     449     165
 
SacII CC_GC'GG

Cuts at:      0      66    1503 
   Size:         66    1437
 
SalI G'TCGA_C

Cuts at:      0      83    1503 
   Size:         83    1420
 
SanDI GG'GwC_CC

Cuts at:      0     270    1445    1503 
   Size:        270    1175      58

  Fragments arranged by size: 

                1175     270      58
 
SexAI A'CCwGG_T

Cuts at:      0     743    1503 
   Size:        743     760
 
SfcI C'TryA_G

Cuts at:      0     255     450     513     541    1339    1503 
   Size:        255     195      63      28     798     164

  Fragments arranged by size: 

                 798     255     195     164      63      28
 
SfoI GGC'GCC

Cuts at:      0      44    1503 
   Size:         44    1459
 
SmaI CCC'GGG

Cuts at:      0     431    1503 
   Size:        431    1072
 
SspI AAT'ATT

Cuts at:      0    1168    1503 
   Size:       1168     335
 
StuI AGG'CCT

Cuts at:      0     551    1503 
   Size:        551     952
 
StyI C'CwwG_G

Cuts at:      0     670     944    1391    1503 
   Size:        670     274     447     112

  Fragments arranged by size: 

                 670     447     274     112
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1030    1503 
   Size:       1030     473
 
TatI w'GTAC_w

Cuts at:      0     230    1503 
   Size:        230    1273
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     307     339    1503 
   Size:        307      32    1164

  Fragments arranged by size: 

                1164     307      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     151    1503 
   Size:        151    1352
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1142    1503 
   Size:       1142     361
 
XmaI C'CCGG_G

Cuts at:      0     429    1503 
   Size:        429    1074
 
XmnI GAAnn'nnTTC

Cuts at:      0     100    1503 
   Size:        100    1403
 

 Enzymes that do cut:

     AccI     AcuI     AleI    AlwNI     ApoI     AvaI    AvrII     BanI
    BanII     BbeI     BbsI    BfrBI     BglI    BglII     BlpI    BmgBI
     BplI     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI
     ClaI     DrdI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NsiI     PasI    PflMI     PfoI    PpuMI
    PshAI     PstI    PvuII    SacII     SalI    SanDI    SexAI     SfcI
     SfoI     SmaI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI Bme1580I     BmrI
     BmtI   Bpu10I    BpuEI    BsaBI    BsiEI    BsiWI     BsmI    BsrFI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     BtsI    CspCI
     DraI   DraIII     EagI  EcoICRI    EcoRI    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     NaeI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PciI     PmeI
     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SwaI     XbaI     XhoI     ZraI
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