Restriction map for pF1KB5237
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DpnII       
DraI        DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        Hin1I       HindIII     KasI        
KpnI        KspI        MaeI        MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MunI        NarI        
NcoI        NdeI        NdeII       NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PleI        
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        TaqI        Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          193  GTCTAC               GT^[AC][GT]AC
AccII          29  CGCG                 CG^CG
AcsI          742  GAATTC               [GA]^AATT[CT]
AgeI          352  ACCGGT               A^CCGGT
ApoI          742  GAATTC               [GA]^AATT[CT]
AspHI         348  GAGCAC               G[AT]GC[AT]^C
AvaII          24  GGTCC                G^G[AT]CC
BbvI          118  GCAGC                GCAGC(8/12)
BglI           79  GCCCAGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          348  GAGCAC               G[AGT]GC[ACT]^C
BpmI          406  CTGGAG               CTGGAG(16/14)
BsiHKAI       348  GAGCAC               G[AT]GC[AT]^C
BsmFI          25  GTCCC                GGGAC(10/14)
Bsp1286I      348  GAGCAC               G[AGT]GC[ACT]^C
BstUI          29  CGCG                 CG^CG
Cac8I          88  GCCGGC               GC[ACGT]^[ACGT]GC
DraII          23  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII        258  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI           86  TGGCCG               [CT]^GGCC[GA]
EarI          696  GAAGAG               CTCTTC(1/4)
EcoO109I       23  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         742  GAATTC               G^AATTC
FauI           27  CCCGC                CCCGC(4/6)
Fnu4HI        118  GCAGC                GC^[ACGT]GC
HaeII          91  GGCGCT               [GA]GCGC^[CT]
HgaI           54  GACGC                GACGC(5/10)
HincII        688  GTTAAC               GT[CT]^[GA]AC
HindII        688  GTTAAC               GT[CT]^[GA]AC
HinfI         672  GATTC                G^A[ACGT]TC
HpaI          688  GTTAAC               GTT^AAC
HphI          272  TCACC                GGTGA(8/7)
ItaI          118  GCAGC                GC^[ACGT]GC
Ksp632I       696  GAAGAG               CTCTTC(1/4)
MaeII         304  ACGT                 A^CGT
MvnI           29  CGCG                 CG^CG
NaeI           88  GCCGGC               GCC^GGC
NgoMIV         88  GCCGGC               G^CCGGC
PinAI         352  ACCGGT               A^CCGGT
PpuMI          23  GGGTCCC              [GA]G^G[AT]CC[CT]
SmaI           20  CCCGGG               CCC^GGG
SspI          753  AATATT               AAT^ATT
TfiI          672  GATTC                G^A[AT]TC
TseI          118  GCAGC                G^C[AT]GC
XmaCI          20  CCCGGG               C^CCGGG
XmaI           20  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AciI           28  CCGC                 CCGC(-3/-1)
               41  CCGC                
AfaI          306  GTAC                 GT^AC
              701  GTAC                
AluI          771  AGCT                 AG^CT
              853  AGCT                
AvaI           20  CCCGGG               C^[CT]CG[GA]G
               34  CCCGAG              
BbsI           73  GTCTTC               GAAGAC(2/6)
              319  GTCTTC              
BpuAI          73  GTCTTC               GAAGAC(2/6)
              319  GTCTTC              
BsaI            4  GAGACC               GGTCTC(1/5)
               37  GAGACC              
BsaWI         352  ACCGGT               [AT]^CCGG[AT]
              692  ACCGGA              
BsoBI          20  CCCGGG               C^[CT]CG[GA]G
               34  CCCGAG              
BsrFI          88  GCCGGC               [GA]^CCGG[CT]
              352  ACCGGT              
CfoI           30  GCGC                 GCG^C
               92  GCGC                
Cfr10I         88  GCCGGC               [GA]^CCGG[CT]
              352  ACCGGT              
DdeI          215  CTAAG                C^T[ACGT]AG
              517  CTAAG               
HhaI           30  GCGC                 GCG^C
               92  GCGC                
HinP1I         30  GCGC                 G^CGC
               92  GCGC                
MaeIII        266  GTGAC                ^GT[ACGT]AC
              469  GTGAC               
MwoI           79  GCCCAGGTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               92  GCGCTTCAGGC         
PshAI          39  GACCGCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              489  GACTTTAGTC          
RsaI          306  GTAC                 GT^AC
              701  GTAC                
Tsp45I        266  GTGAC                ^GT[GC]AC
              469  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

BcnI        BsaJI       BsiYI       BslI        BsmAI       
BssKI       BstF5I      BstNI       Cfr13I      EcoRII      
FokI        HaeIII      HapII       HpaII       MboII       
MnlI        MseI        MspI        MvaI        NciI        
NlaIV       Sau96I      ScrFI       Tru9I       Tsp509I     
TspRI       
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