Restriction map for pF1KB5237
 (Linear) (Six-base) MAPSORT of: KIBB5237.seq  Check: 5009  from: 1  to: 885



KIBB5237.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AccI GT'mk_AC

Cuts at:      0     194     885 
   Size:        194     691
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     885 
   Size:         80     805
 
AgeI A'CCGG_T

Cuts at:      0     352     885 
   Size:        352     533
 
ApoI r'AATT_y

Cuts at:      0     742     885 
   Size:        742     143
 
AvaI C'yCGr_G

Cuts at:      0      20      34     885 
   Size:         20      14     851

  Fragments arranged by size: 

                 851      20      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      66     312     885 
   Size:         66     246     573

  Fragments arranged by size: 

                 573     246      66
 
BglI GCCn_nnn'nGGC

Cuts at:      0      85     885 
   Size:         85     800
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     885 
   Size:        427     458
 
Bpu10I CC'TnA_GC

Cuts at:      0     517     885 
   Size:        517     368
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      31     885 
   Size:         31     854
 
BsaWI w'CCGG_w

Cuts at:      0     352     692     885 
   Size:        352     340     193

  Fragments arranged by size: 

                 352     340     193
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     358     388     761     791     885 
   Size:        358      30     373      30      94

  Fragments arranged by size: 

                 373     358      94      30      30
 
BseYI C'CCAG_C

Cuts at:      0      68     361     885 
   Size:         68     293     524

  Fragments arranged by size: 

                 524     293      68
 
BsiHKAI G_wGCw'C

Cuts at:      0     352     885 
   Size:        352     533
 
Bsp1286I G_dGCh'C

Cuts at:      0     352     885 
   Size:        352     533
 
BsrFI r'CCGG_y

Cuts at:      0      88     352     885 
   Size:         88     264     533

  Fragments arranged by size: 

                 533     264      88
 
BtsI GCAGTG_nn'

Cuts at:      0     674     885 
   Size:        674     211
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      95     130     885 
   Size:         95      35     755

  Fragments arranged by size: 

                 755      95      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     263     885 
   Size:        263     622
 
EaeI y'GGCC_r

Cuts at:      0      86     885 
   Size:         86     799
 
EarI CTCTTCn'nnn_

Cuts at:      0     691     885 
   Size:        691     194
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     427     885 
   Size:         80     347     458

  Fragments arranged by size: 

                 458     347      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24     885 
   Size:         24     861
 
EcoRI G'AATT_C

Cuts at:      0     742     885 
   Size:        742     143
 
HaeII r_GCGC'y

Cuts at:      0      95     885 
   Size:         95     790
 
HincII GTy'rAC

Cuts at:      0     690     885 
   Size:        690     195
 
HpaI GTT'AAC

Cuts at:      0     690     885 
   Size:        690     195
 
NaeI GCC'GGC

Cuts at:      0      90     885 
   Size:         90     795
 
NgoMIV G'CCGG_C

Cuts at:      0      88     885 
   Size:         88     797
 
PfoI T'CCnGG_A

Cuts at:      0     524     885 
   Size:        524     361
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     339     371     885 
   Size:        339      32     514

  Fragments arranged by size: 

                 514     339      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      24     885 
   Size:         24     861
 
PshAI GACnn'nnGTC

Cuts at:      0      43     493     885 
   Size:         43     450     392

  Fragments arranged by size: 

                 450     392      43
 
SanDI GG'GwC_CC

Cuts at:      0      24     885 
   Size:         24     861
 
SmaI CCC'GGG

Cuts at:      0      22     885 
   Size:         22     863
 
SspI AAT'ATT

Cuts at:      0     755     885 
   Size:        755     130
 
TatI w'GTAC_w

Cuts at:      0     700     885 
   Size:        700     185
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     446     478     885 
   Size:        446      32     407

  Fragments arranged by size: 

                 446     407      32
 
XmaI C'CCGG_G

Cuts at:      0      20     885 
   Size:         20     865
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     ApoI     AvaI     BbsI     BglI     BpmI
   Bpu10I     BsaI    BsaWI    BsaXI    BseYI  BsiHKAI Bsp1286I    BsrFI
     BtsI    CspCI   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRI
    HaeII   HincII     HpaI     NaeI   NgoMIV     PfoI     PpiI    PpuMI
    PshAI    SanDI     SmaI     SspI     TatI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI     DraI
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI
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