Restriction map for pF1KB6642
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvrII       BamHI       BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    EcoNI       EcoRV       
EcoT22I     FbaI        FseI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MroI        MseI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         1102  GTCTAC               GT^[AC][GT]AC
AcsI         1260  GAATTC               [GA]^AATT[CT]
AflIII       1076  ACGTGT               A^C[GA][CT]GT
Aor51HI       644  AGCGCT               AGC^GCT
ApoI         1260  GAATTC               [GA]^AATT[CT]
AviII         870  TGCGCA               TGC^GCA
BalI          827  TGGCCA               TGG^CCA
BanI         1111  GGTGCC               G^G[CT][GA]CC
BcgI          393  GCACGACTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI        1231  TACGTG               [CT]AC^GT[GA]
BseRI          77  CTCCTC               GAGGAG(10/8)
BsgI          391  CTGCAC               GTGCAG(16/14)
BsiEI        1207  CGGCCG               CG[GA][CT]^CG
BsmI          112  GAATGC               GAATGC(1/-1)
BspHI        1268  TCATGA               T^CATGA
BsrFI        1209  GCCGGT               [GA]^CCGG[CT]
Cfr10I       1209  GCCGGT               [GA]^CCGG[CT]
DsaI          909  CCATGG               C^C[GA][CT]GG
EagI         1207  CGGCCG               C^GGCCG
EarI           91  GAAGAG               CTCTTC(1/4)
EclXI        1207  CGGCCG               C^GGCCG
Eco47III      644  AGCGCT               AGC^GCT
Eco52I       1207  CGGCCG               C^GGCCG
EcoRI        1260  GAATTC               G^AATTC
EcoT14I       909  CCATGG               C^C[AT][AT]GG
FspI          870  TGCGCA               TGC^GCA
HgaI          773  GCGTC                GACGC(5/10)
Ksp632I        91  GAAGAG               CTCTTC(1/4)
MboII          91  GAAGA                GAAGA(8/7)
MluNI         827  TGGCCA               TGG^CCA
MscI          827  TGGCCA               TGG^CCA
MslI          703  CATCGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          909  CCATGG               C^CATGG
PflMI         819  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          862  GAGTC                GAGTC(4/5)
PshAI        1095  GACCTCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          720  CTGCAG               CTGCA^G
RcaI         1268  TCATGA               T^CATGA
SapI           91  GAAGAGC              GCTCTTC(1/4)
SfcI          720  CTGCAG               C^T[GA][CT]AG
StuI          188  AGGCCT               AGG^CCT
StyI          909  CCATGG               C^C[AT][AT]GG
TaqI         1008  TCGA                 T^CGA
TfiI           85  GATTC                G^A[AT]TC
Tsp509I      1261  AATT                 ^AATT
TthHB8I      1008  TCGA                 T^CGA
Van91I        819  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Alw44I        444  GTGCAC               G^TGCAC
             1030  GTGCAC              
AlwNI         385  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              820  CAGCTGCTG           
AocI           79  CCTCAGG              CC^T[ACGT]AGG
              184  CCTGAGG             
ApaLI         444  GTGCAC               G^TGCAC
             1030  GTGCAC              
BglI          326  GCCCACCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              683  GCCACCAGGGC         
BpmI          747  CTCCAG               CTGGAG(16/14)
             1243  CTGGAG              
BsaI           29  GGTCTC               GGTCTC(1/5)
              963  GGTCTC              
BspMI         448  ACCTGC               ACCTGC(4/8)
              536  ACCTGC              
BstYI        1085  AGATCC               [GA]^GATC[CT]
             1130  AGATCC              
Bsu36I         79  CCTCAGG              CC^T[ACGT]AGG
              184  CCTGAGG             
EaeI          827  TGGCCA               [CT]^GGCC[GA]
             1207  CGGCCG              
Eco81I         79  CCTCAGG              CC^T[ACGT]AGG
              184  CCTGAGG             
HinfI          85  GATTC                G^A[ACGT]TC
              862  GAGTC               
MflI         1085  AGATCC               [GA]^GATC[CT]
             1130  AGATCC              
SfaNI         212  GCATC                GCATC(5/9)
              702  GCATC               
SmaI           25  CCCGGG               CCC^GGG
             1223  CCCGGG              
XcmI          420  CCATAACTTGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              935  CCAACCGCTGCGTGG     
XhoII        1085  AGATCC               [GA]^GATC[CT]
             1130  AGATCC              
XmaCI          25  CCCGGG               C^CCGGG
             1223  CCCGGG              
XmaI           25  CCCGGG               C^CCGGG
             1223  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        ApaI        
AspHI       AvaI        AvaII       BanII       BbvI        
BcnI        BmyI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrBI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoO65I     
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       TseI        Tsp45I      TspRI       
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