Restriction map for pF1KB6642
 (Linear) (Six-base) MAPSORT of: KIBB6642.seq  Check: 2822  from: 1  to: 1329



KIBB6642.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     457    1329 
   Size:        457     872
 
AccI GT'mk_AC

Cuts at:      0    1103    1329 
   Size:       1103     226
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110    1329 
   Size:        110    1219
 
AfeI AGC'GCT

Cuts at:      0     646    1329 
   Size:        646     683
 
AflIII A'CryG_T

Cuts at:      0    1076    1329 
   Size:       1076     253
 
AlwNI CAG_nnn'CTG

Cuts at:      0     390     825    1329 
   Size:        390     435     504

  Fragments arranged by size: 

                 504     435     390
 
ApaI G_GGCC'C

Cuts at:      0     479     633    1222    1329 
   Size:        479     154     589     107

  Fragments arranged by size: 

                 589     479     154     107
 
ApaLI G'TGCA_C

Cuts at:      0     444    1030    1329 
   Size:        444     586     299

  Fragments arranged by size: 

                 586     444     299
 
ApoI r'AATT_y

Cuts at:      0    1260    1329 
   Size:       1260      69
 
AvaI C'yCGr_G

Cuts at:      0      25     478    1223    1329 
   Size:         25     453     745     106

  Fragments arranged by size: 

                 745     453     106      25
 
BanI G'GyrC_C

Cuts at:      0    1111    1329 
   Size:       1111     218
 
BanII G_rGCy'C

Cuts at:      0      98     344     479     633     953    1222    1329 
   Size:         98     246     135     154     320     269     107

  Fragments arranged by size: 

                 320     269     246     154     135     107      98
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     382     416    1329 
   Size:        382      34     913

  Fragments arranged by size: 

                 913     382      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     332     689    1329 
   Size:        332     357     640

  Fragments arranged by size: 

                 640     357     332
 
Bme1580I G_kGCm'C

Cuts at:      0     384     448     479     633    1034    1070    1222    1329 
   Size:        384      64      31     154     401      36     152     107

  Fragments arranged by size: 

                 401     384     154     152     107      64      36      31
            
 
BmgBI CAC'GTC

Cuts at:      0    1077    1329 
   Size:       1077     252
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     732    1264    1329 
   Size:        732     532      65

  Fragments arranged by size: 

                 732     532      65
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      35     969    1329 
   Size:         35     934     360

  Fragments arranged by size: 

                 934     360      35
 
BsaAI yAC'GTr

Cuts at:      0    1233    1329 
   Size:       1233      96
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     580     610     865     895    1329 
   Size:        580      30     255      30     434

  Fragments arranged by size: 

                 580     434     255      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      68    1329 
   Size:         68    1261
 
BseYI C'CCAG_C

Cuts at:      0     406     579    1329 
   Size:        406     173     750

  Fragments arranged by size: 

                 750     406     173
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376    1329 
   Size:        376     953
 
BsiEI CG_ry'CG

Cuts at:      0    1210    1329 
   Size:       1210     119
 
BsiHKAI G_wGCw'C

Cuts at:      0     448     883    1034    1329 
   Size:        448     435     151     295

  Fragments arranged by size: 

                 448     435     295     151
 
BsmI GAATG_Cn'

Cuts at:      0     118    1329 
   Size:        118    1211
 
Bsp1286I G_dGCh'C

Cuts at:      0      98     344     384     448     479     633     883     953 
   Size:         98     246      40      64      31     154     250      70

Cuts at:    953    1034    1070    1222    1329 
   Size:         81      36     152     107

  Fragments arranged by size: 

                 250     246     154     152     107      98      81      70
                  64      40      36      31
 
BspHI T'CATG_A

Cuts at:      0    1268    1329 
   Size:       1268      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     457     545    1329 
   Size:        457      88     784

  Fragments arranged by size: 

                 784     457      88
 
BsrBI CCG'CTC

Cuts at:      0     248     307     596    1059    1329 
   Size:        248      59     289     463     270

  Fragments arranged by size: 

                 463     289     270     248      59
 
BsrFI r'CCGG_y

Cuts at:      0    1209    1329 
   Size:       1209     120
 
BstEII G'GTnAC_C

Cuts at:      0     460     814    1251    1329 
   Size:        460     354     437      78

  Fragments arranged by size: 

                 460     437     354      78
 
BstYI r'GATC_y

Cuts at:      0    1085    1130    1329 
   Size:       1085      45     199

  Fragments arranged by size: 

                1085     199      45
 
Bsu36I CC'TnA_GG

Cuts at:      0      80     185    1329 
   Size:         80     105    1144

  Fragments arranged by size: 

                1144     105      80
 
BtgI C'CryG_G

Cuts at:      0     909    1329 
   Size:        909     420
 
BtsI GCAGTG_nn'

Cuts at:      0     150     729     801    1329 
   Size:        150     579      72     528

  Fragments arranged by size: 

                 579     528     150      72
 
EaeI y'GGCC_r

Cuts at:      0     827    1207    1329 
   Size:        827     380     122

  Fragments arranged by size: 

                 827     380     122
 
EagI C'GGCC_G

Cuts at:      0    1207    1329 
   Size:       1207     122
 
EarI CTCTTCn'nnn_

Cuts at:      0      86    1329 
   Size:         86    1243
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     732    1264    1329 
   Size:        110     622     532      65

  Fragments arranged by size: 

                 622     532     110      65
 
EcoO109I rG'GnC_Cy

Cuts at:      0     135     283     387     583     629     630     665    1219 
   Size:        135     148     104     196      46       1      35     554

Cuts at:   1219    1316    1329 
   Size:         97      13

  Fragments arranged by size: 

                 554     196     148     135     104      97      46      35
                  13       1
 
EcoRI G'AATT_C

Cuts at:      0    1260    1329 
   Size:       1260      69
 
FspI TGC'GCA

Cuts at:      0     872    1329 
   Size:        872     457
 
FspAI rTGC'GCAy

Cuts at:      0     872    1329 
   Size:        872     457
 
HaeII r_GCGC'y

Cuts at:      0      49     648    1190    1329 
   Size:         49     599     542     139

  Fragments arranged by size: 

                 599     542     139      49
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     700     732     847     879    1329 
   Size:        700      32     115      32     450

  Fragments arranged by size: 

                 700     450     115      32      32
 
HincII GTy'rAC

Cuts at:      0     165     777     903    1329 
   Size:        165     612     126     426

  Fragments arranged by size: 

                 612     426     165     126
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1019    1329 
   Size:       1019     310
 
MscI TGG'CCA

Cuts at:      0     829    1329 
   Size:        829     500
 
MslI CAynn'nnrTG

Cuts at:      0     707    1329 
   Size:        707     622
 
MspA1I CmG'CkG

Cuts at:      0     253     359     527     554     579     770     822     923 
   Size:        253     106     168      27      25     191      52     101

Cuts at:    923     941     977    1072    1329 
   Size:         18      36      95     257

  Fragments arranged by size: 

                 257     253     191     168     106     101      95      52
                  36      27      25      18
 
NcoI C'CATG_G

Cuts at:      0     909    1329 
   Size:        909     420
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     825    1329 
   Size:        825     504
 
PpuMI rG'GwC_Cy

Cuts at:      0     135     283     665    1329 
   Size:        135     148     382     664

  Fragments arranged by size: 

                 664     382     148     135
 
PshAI GACnn'nnGTC

Cuts at:      0    1099    1329 
   Size:       1099     230
 
PspOMI G'GGCC_C

Cuts at:      0     475     629    1218    1329 
   Size:        475     154     589     111

  Fragments arranged by size: 

                 589     475     154     111
 
PstI C_TGCA'G

Cuts at:      0     724    1329 
   Size:        724     605
 
PvuII CAG'CTG

Cuts at:      0     359     579     822    1329 
   Size:        359     220     243     507

  Fragments arranged by size: 

                 507     359     243     220
 
SanDI GG'GwC_CC

Cuts at:      0     135     283    1329 
   Size:        135     148    1046

  Fragments arranged by size: 

                1046     148     135
 
SapI GCTCTTCn'nnn_

Cuts at:      0      86    1329 
   Size:         86    1243
 
SfcI C'TryA_G

Cuts at:      0     720    1329 
   Size:        720     609
 
SmaI CCC'GGG

Cuts at:      0      27    1225    1329 
   Size:         27    1198     104

  Fragments arranged by size: 

                1198     104      27
 
StuI AGG'CCT

Cuts at:      0     190    1329 
   Size:        190    1139
 
StyI C'CwwG_G

Cuts at:      0     909    1329 
   Size:        909     420
 
TatI w'GTAC_w

Cuts at:      0    1290    1329 
   Size:       1290      39
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     193     225     865     897    1329 
   Size:        193      32     640      32     432

  Fragments arranged by size: 

                 640     432     193      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     427     942    1329 
   Size:        427     515     387

  Fragments arranged by size: 

                 515     427     387
 
XmaI C'CCGG_G

Cuts at:      0      25    1223    1329 
   Size:         25    1198     106

  Fragments arranged by size: 

                1198     106      25
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII     BcgI     BglI Bme1580I    BmgBI
     BpmI     BsaI    BsaAI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI   BstEII
    BstYI   Bsu36I     BtgI     BtsI     EaeI     EagI     EarI  Eco57MI
 EcoO109I    EcoRI     FspI    FspAI    HaeII    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NcoI    PflMI    PpuMI    PshAI   PspOMI
     PstI    PvuII    SanDI     SapI     SfcI     SmaI     StuI     StyI
     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI     BmrI
     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI    BsiWI
    BsmBI    BspEI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmnI     ZraI
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