Restriction map for pF1KB3507
 (Linear) (Six-base) MAPSORT of: KIBB3507.seq  Check: 5300  from: 1  to: 1119



KIBB3507.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      33    1119 
   Size:         33    1086
 
AatII G_ACGT'C

Cuts at:      0     897    1119 
   Size:        897     222
 
AccI GT'mk_AC

Cuts at:      0     409    1119 
   Size:        409     710
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     750     820    1119 
   Size:        750      70     299

  Fragments arranged by size: 

                 750     299      70
 
AflIII A'CryG_T

Cuts at:      0      50     227    1072    1119 
   Size:         50     177     845      47

  Fragments arranged by size: 

                 845     177      50      47
 
AleI CACnn'nnGTG

Cuts at:      0     854    1119 
   Size:        854     265
 
ApaI G_GGCC'C

Cuts at:      0     470    1119 
   Size:        470     649
 
ApoI r'AATT_y

Cuts at:      0     180     607    1119 
   Size:        180     427     512

  Fragments arranged by size: 

                 512     427     180
 
BanI G'GyrC_C

Cuts at:      0     431     839    1119 
   Size:        431     408     280

  Fragments arranged by size: 

                 431     408     280
 
BanII G_rGCy'C

Cuts at:      0     152     470    1103    1119 
   Size:        152     318     633      16

  Fragments arranged by size: 

                 633     318     152      16
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     695    1069    1119 
   Size:        695     374      50

  Fragments arranged by size: 

                 695     374      50
 
BbvCI CC'TCA_GC

Cuts at:      0       9    1119 
   Size:          9    1110
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     504     538     919     953    1119 
   Size:        504      34     381      34     166

  Fragments arranged by size: 

                 504     381     166      34      34
 
Bme1580I G_kGCm'C

Cuts at:      0     434     470    1119 
   Size:        434      36     649

  Fragments arranged by size: 

                 649     434      36
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     594    1119 
   Size:        594     525
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     667    1119 
   Size:        667     452
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     177     578    1119 
   Size:        128      49     401     541

  Fragments arranged by size: 

                 541     401     128      49
 
Bpu10I CC'TnA_GC

Cuts at:      0       9     719    1119 
   Size:          9     710     400

  Fragments arranged by size: 

                 710     400       9
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     322    1119 
   Size:        322     797
 
BsaAI yAC'GTr

Cuts at:      0    1073    1119 
   Size:       1073      46
 
BsaHI Gr'CG_yC

Cuts at:      0     894    1119 
   Size:        894     225
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     391     421    1119 
   Size:        391      30     698

  Fragments arranged by size: 

                 698     391      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     802     826    1119 
   Size:        802      24     293

  Fragments arranged by size: 

                 802     293      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     767    1119 
   Size:        767     352
 
BsmI GAATG_Cn'

Cuts at:      0     971    1119 
   Size:        971     148
 
Bsp1286I G_dGCh'C

Cuts at:      0     152     434     470    1103    1119 
   Size:        152     282      36     633      16

  Fragments arranged by size: 

                 633     282     152      36      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      33    1119 
   Size:         33    1086
 
BsrFI r'CCGG_y

Cuts at:      0    1090    1119 
   Size:       1090      29
 
BsrGI T'GTAC_A

Cuts at:      0      47    1119 
   Size:         47    1072
 
BssSI C'ACGA_G

Cuts at:      0     235    1119 
   Size:        235     884
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     159    1119 
   Size:        159     960
 
BstZ17I GTA'TAC

Cuts at:      0     410    1119 
   Size:        410     709
 
Bsu36I CC'TnA_GG

Cuts at:      0      62    1119 
   Size:         62    1057
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1109    1119 
   Size:       1109      10
 
ClaI AT'CG_AT

Cuts at:      0      98    1119 
   Size:         98    1021
 
EaeI y'GGCC_r

Cuts at:      0     987    1119 
   Size:        987     132
 
EarI CTCTTCn'nnn_

Cuts at:      0     158    1119 
   Size:        158     961
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     177     578     750     820    1119 
   Size:        128      49     401     172      70     299

  Fragments arranged by size: 

                 401     299     172     128      70      49
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      61     513     626    1119 
   Size:         61     452     113     493

  Fragments arranged by size: 

                 493     452     113      61
 
EcoO109I rG'GnC_Cy

Cuts at:      0     258     273     466     467    1119 
   Size:        258      15     193       1     652

  Fragments arranged by size: 

                 652     258     193      15       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1023    1119 
   Size:       1023      96
 
HaeII r_GCGC'y

Cuts at:      0     962    1119 
   Size:        962     157
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     476     944    1119 
   Size:        242     234     468     175

  Fragments arranged by size: 

                 468     242     234     175
 
MscI TGG'CCA

Cuts at:      0     989    1119 
   Size:        989     130
 
MslI CAynn'nnrTG

Cuts at:      0     854    1119 
   Size:        854     265
 
NspI r_CATG'y

Cuts at:      0      54     231    1119 
   Size:         54     177     888

  Fragments arranged by size: 

                 888     177      54
 
PasI CC'CwG_GG

Cuts at:      0     463     471    1119 
   Size:        463       8     648

  Fragments arranged by size: 

                 648     463       8
 
PciI A'CATG_T

Cuts at:      0      50     227    1119 
   Size:         50     177     892

  Fragments arranged by size: 

                 892     177      50
 
PmlI CAC'GTG

Cuts at:      0    1073    1119 
   Size:       1073      46
 
PpuMI rG'GwC_Cy

Cuts at:      0     258     273    1119 
   Size:        258      15     846

  Fragments arranged by size: 

                 846     258      15
 
PspOMI G'GGCC_C

Cuts at:      0     466    1119 
   Size:        466     653
 
PstI C_TGCA'G

Cuts at:      0     327    1119 
   Size:        327     792
 
SanDI GG'GwC_CC

Cuts at:      0     258    1119 
   Size:        258     861
 
ScaI AGT'ACT

Cuts at:      0     198    1119 
   Size:        198     921
 
SfcI C'TryA_G

Cuts at:      0     323    1119 
   Size:        323     796
 
StuI AGG'CCT

Cuts at:      0     321     878    1119 
   Size:        321     557     241

  Fragments arranged by size: 

                 557     321     241
 
StyI C'CwwG_G

Cuts at:      0     773     869    1119 
   Size:        773      96     250

  Fragments arranged by size: 

                 773     250      96
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     645    1119 
   Size:        645     474
 
TatI w'GTAC_w

Cuts at:      0      47     196     308     803    1109    1119 
   Size:         47     149     112     495     306      10

  Fragments arranged by size: 

                 495     306     149     112      47      10
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     876    1119 
   Size:        876     243
 
ZraI GAC'GTC

Cuts at:      0     895    1119 
   Size:        895     224
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     ApaI     ApoI
     BanI    BanII     BbsI    BbvCI     BcgI Bme1580I     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI    BseRI     BsgI
     BsmI Bsp1286I    BspMI    BsrFI    BsrGI    BssSI    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII     MmeI     MscI     MslI     NspI     PasI     PciI
     PmlI    PpuMI   PspOMI     PstI    SanDI     ScaI     SfcI     StuI
     StyI    TaqII     TatI     XcmI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI     AloI
    AlwNI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI    BpuEI    BsaBI    BsaWI    BseYI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI   BssHII   BstAPI
    BstBI   BstEII    BstYI     BtgI     BtsI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
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