Restriction map for pF1KB6694
 (Linear) (Six-base) MAPSORT of: KIBB6694.seq  Check: 2454  from: 1  to: 1386



KIBB6694.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AccI GT'mk_AC

Cuts at:      0     758    1386 
   Size:        758     628
 
AclI AA'CG_TT

Cuts at:      0     851     971    1386 
   Size:        851     120     415

  Fragments arranged by size: 

                 851     415     120
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1144    1386 
   Size:       1144     242
 
AflIII A'CryG_T

Cuts at:      0      42    1386 
   Size:         42    1344
 
ApoI r'AATT_y

Cuts at:      0     121     235     364    1174    1386 
   Size:        121     114     129     810     212

  Fragments arranged by size: 

                 810     212     129     121     114
 
AvaI C'yCGr_G

Cuts at:      0     398    1386 
   Size:        398     988
 
BanII G_rGCy'C

Cuts at:      0     157    1386 
   Size:        157    1229
 
BglI GCCn_nnn'nGGC

Cuts at:      0     415    1386 
   Size:        415     971
 
BlpI GC'TnA_GC

Cuts at:      0     194    1118    1386 
   Size:        194     924     268

  Fragments arranged by size: 

                 924     268     194
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     436    1386 
   Size:        436     950
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     920    1260    1386 
   Size:        920     340     126

  Fragments arranged by size: 

                 920     340     126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     733     827    1358    1386 
   Size:        625     108      94     531      28

  Fragments arranged by size: 

                 625     531     108      94      28
 
Bpu10I CC'TnA_GC

Cuts at:      0    1025    1386 
   Size:       1025     361
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     706    1386 
   Size:        706     680
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     235    1386 
   Size:        235    1151
 
BsaAI yAC'GTr

Cuts at:      0      45    1386 
   Size:         45    1341
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     825     855    1386 
   Size:        825      30     531

  Fragments arranged by size: 

                 825     531      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     157    1386 
   Size:        157    1229
 
BsrDI GCAATG_nn'

Cuts at:      0     669    1100    1386 
   Size:        669     431     286

  Fragments arranged by size: 

                 669     431     286
 
BsrFI r'CCGG_y

Cuts at:      0    1063    1386 
   Size:       1063     323
 
BstEII G'GTnAC_C

Cuts at:      0     831    1350    1386 
   Size:        831     519      36

  Fragments arranged by size: 

                 831     519      36
 
BtsI GCAGTG_nn'

Cuts at:      0    1019    1325    1386 
   Size:       1019     306      61

  Fragments arranged by size: 

                1019     306      61
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     651     666     686     701     750     785    1386 
   Size:        651      15      20      15      49      35     601

  Fragments arranged by size: 

                 651     601      49      35      20      15      15
 
DraIII CAC_nnn'GTG

Cuts at:      0     430    1386 
   Size:        430     956
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     334    1386 
   Size:        334    1052
 
EaeI y'GGCC_r

Cuts at:      0      72     792    1386 
   Size:         72     720     594

  Fragments arranged by size: 

                 720     594      72
 
EarI CTCTTCn'nnn_

Cuts at:      0     487     911    1386 
   Size:        487     424     475

  Fragments arranged by size: 

                 487     475     424
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     733     827    1144    1358    1386 
   Size:        625     108      94     317     214      28

  Fragments arranged by size: 

                 625     317     214     108      94      28
 
EcoRV GAT'ATC

Cuts at:      0     222    1386 
   Size:        222    1164
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     174    1386 
   Size:        174    1212
 
HaeII r_GCGC'y

Cuts at:      0    1064    1386 
   Size:       1064     322
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     278     310     629     661    1386 
   Size:        278      32     319      32     725

  Fragments arranged by size: 

                 725     319     278      32      32
 
HincII GTy'rAC

Cuts at:      0     456     849    1386 
   Size:        456     393     537

  Fragments arranged by size: 

                 537     456     393
 
HindIII A'AGCT_T

Cuts at:      0     890    1386 
   Size:        890     496
 
HpaI GTT'AAC

Cuts at:      0     456    1386 
   Size:        456     930
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     565     745    1386 
   Size:        565     180     641

  Fragments arranged by size: 

                 641     565     180
 
MscI TGG'CCA

Cuts at:      0      74    1386 
   Size:         74    1312
 
MspA1I CmG'CkG

Cuts at:      0    1012    1386 
   Size:       1012     374
 
NaeI GCC'GGC

Cuts at:      0    1065    1386 
   Size:       1065     321
 
NgoMIV G'CCGG_C

Cuts at:      0    1063    1386 
   Size:       1063     323
 
NspI r_CATG'y

Cuts at:      0     409     603     624    1109    1386 
   Size:        409     194      21     485     277

  Fragments arranged by size: 

                 485     409     277     194      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     580    1386 
   Size:        580     806
 
PfoI T'CCnGG_A

Cuts at:      0     749    1386 
   Size:        749     637
 
PvuII CAG'CTG

Cuts at:      0    1012    1386 
   Size:       1012     374
 
SapI GCTCTTCn'nnn_

Cuts at:      0     911    1386 
   Size:        911     475
 
ScaI AGT'ACT

Cuts at:      0     253    1386 
   Size:        253    1133
 
SfcI C'TryA_G

Cuts at:      0     483    1386 
   Size:        483     903
 
SmlI C'TyrA_G

Cuts at:      0     685    1386 
   Size:        685     701
 
SphI G_CATG'C

Cuts at:      0     409    1109    1386 
   Size:        409     700     277

  Fragments arranged by size: 

                 700     409     277
 
StyI C'CwwG_G

Cuts at:      0     625    1386 
   Size:        625     761
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     418    1294    1386 
   Size:        418     876      92

  Fragments arranged by size: 

                 876     418      92
 
TatI w'GTAC_w

Cuts at:      0     251    1386 
   Size:        251    1135
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     825     857    1386 
   Size:        825      32     529

  Fragments arranged by size: 

                 825     529      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     581    1206    1386 
   Size:        581     625     180

  Fragments arranged by size: 

                 625     581     180
 
XmnI GAAnn'nnTTC

Cuts at:      0     905    1386 
   Size:        905     481
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     ApoI     AvaI    BanII     BglI
     BlpI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaXI Bsp1286I    BsrDI    BsrFI   BstEII     BtsI    CspCI   DraIII
     DrdI     EaeI     EarI  Eco57MI    EcoRV     FalI    HaeII    Hin4I
   HincII  HindIII     HpaI     MmeI     MscI   MspA1I     NaeI   NgoMIV
     NspI    PflMI     PfoI    PvuII     SapI     ScaI     SfcI     SmlI
     SphI     StyI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII Bme1580I    BmgBI     BmtI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI     DraI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI     FseI     FspI    FspAI     KasI     KpnI     MfeI     MluI
     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XhoI     XmaI     ZraI
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