Restriction map for pF1KE1125
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspI        AvrII       BamHI       BcgI        
BciVI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BsaBI       BsiWI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DrdI        EarI        Eco47III    
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HgaI        HindIII     HpaI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MseI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Tth111I     Van91I      XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII        360  TCCGGA               T^CCGGA
AcyI         1287  GGCGCC               G[GA]^CG[CT]C
AhdI          445  GACTCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         619  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI         445  GACTCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        1245  TGCGCA               TGC^GCA
BalI          530  TGGCCA               TGG^CCA
BbeI         1287  GGCGCC               GGCGC^C
BbrPI        1675  CACGTG               CAC^GTG
BbsI          205  GAAGAC               GAAGAC(2/6)
BbvCI        1365  GCTGAGG              CCTCAGC(-5/-2)
BglII         335  AGATCT               A^GATCT
BpuAI         205  GAAGAC               GAAGAC(2/6)
BsaAI        1675  CACGTG               [CT]AC^GT[GA]
BsaHI        1287  GGCGCC               G[GA]^CG[CT]C
BsaI           39  GGTCTC               GGTCTC(1/5)
BsaWI         360  TCCGGA               [AT]^CCGG[AT]
BseAI         360  TCCGGA               T^CCGGA
BsgI          973  GTGCAG               GTGCAG(16/14)
BsmBI          17  CGTCTC               CGTCTC(1/5)
BspEI         360  TCCGGA               T^CCGGA
BssHII        159  GCGCGC               G^CGCGC
DraIII        988  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1373  CCACGG               C^C[GA][CT]GG
EagI         1152  CGGCCG               C^GGCCG
Eam1105I      445  GACTCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1152  CGGCCG               C^GGCCG
Eco52I       1152  CGGCCG               C^GGCCG
EcoT14I       595  CCTTGG               C^C[AT][AT]GG
FspI         1245  TGCGCA               TGC^GCA
HaeII        1287  GGCGCC               [GA]GCGC^[CT]
Hin1I        1287  GGCGCC               G[GA]^CG[CT]C
HincII       1138  GTCAAC               GT[CT]^[GA]AC
HindII       1138  GTCAAC               GT[CT]^[GA]AC
KasI         1287  GGCGCC               G^GCGCC
MaeII        1676  ACGT                 A^CGT
MluNI         530  TGGCCA               TGG^CCA
MroI          360  TCCGGA               T^CCGGA
MscI          530  TGGCCA               TGG^CCA
NarI         1287  GGCGCC               GG^CGCC
NspI         1361  GCATGC               [GA]CATG^[CT]
PmaCI        1675  CACGTG               CAC^GTG
PmlI         1675  CACGTG               CAC^GTG
PstI          566  CTGCAG               CTGCA^G
PvuI          296  CGATCG               CGAT^CG
SexAI        1019  ACCAGGT              A^CC[AT]GGT
SfoI         1287  GGCGCC               GGC^GCC
SphI         1361  GCATGC               GCATG^C
StuI         1382  AGGCCT               AGG^CCT
StyI          595  CCTTGG               C^C[AT][AT]GG
Tsp509I       884  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwI          484  GATCC                GGATC(4/5)
              859  GGATC               
AocI          379  CCTGAGG              CC^T[ACGT]AGG
              694  CCTCAGG             
AvaII         699  GGTCC                G^G[AT]CC
             1452  GGACC               
BfaI         1201  CTAG                 C^TAG
             1703  CTAG                
BglI          173  GCCGGGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              753  GCCCCGAGGGC         
BsiEI         296  CGATCG               CG[GA][CT]^CG
             1152  CGGCCG              
BspMI        1231  ACCTGC               ACCTGC(4/8)
             1336  ACCTGC              
BsrFI        1002  ACCGGC               [GA]^CCGG[CT]
             1463  ACCGGC              
BstYI         335  AGATCT               [GA]^GATC[CT]
              859  GGATCT              
Bsu36I        379  CCTGAGG              CC^T[ACGT]AGG
              694  CCTCAGG             
Cfr10I       1002  ACCGGC               [GA]^CCGG[CT]
             1463  ACCGGC              
Eco81I        379  CCTGAGG              CC^T[ACGT]AGG
              694  CCTCAGG             
MaeI         1201  CTAG                 C^TAG
             1703  CTAG                
MflI          335  AGATCT               [GA]^GATC[CT]
              859  GGATCT              
MslI         1237  CACCAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1404  CACCTGTGTG          
MspA1I       1081  CAGCTG               C[AC]G^C[GT]G
             1207  CAGCTG              
PvuII        1081  CAGCTG               CAG^CTG
             1207  CAGCTG              
SfcI          566  CTGCAG               C^T[GA][CT]AG
             1272  CTACAG              
XhoII         335  AGATCT               [GA]^GATC[CT]
              859  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        ApaI        
Asp700I     AspHI       AvaI        BanI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmaI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     XmaCI       
XmaI        XmnI        
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