Restriction map for pF1KE1125
 (Linear) (Six-base) MAPSORT of: KIEE1125.seq  Check: 9217  from: 1  to: 1716



KIEE1125.orf

 With 182 enzymes: SgfI * 

                        March 23, 2015 09:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1240    1345    1716 
   Size:       1240     105     371

  Fragments arranged by size: 

                1240     371     105
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1078    1716 
   Size:       1078     638
 
AhdI GACnn_n'nnGTC

Cuts at:      0     450    1716 
   Size:        450    1266
 
AleI CACnn'nnGTG

Cuts at:      0    1241    1408    1716 
   Size:       1241     167     308

  Fragments arranged by size: 

                1241     308     167
 
AlwNI CAG_nnn'CTG

Cuts at:      0     624    1716 
   Size:        624    1092
 
ApaI G_GGCC'C

Cuts at:      0     156     764    1041    1049    1716 
   Size:        156     608     277       8     667

  Fragments arranged by size: 

                 667     608     277     156       8
 
AvaI C'yCGr_G

Cuts at:      0     121     148     497     755     968    1175    1503    1716 
   Size:        121      27     349     258     213     207     328     213

  Fragments arranged by size: 

                 349     328     258     213     213     207     121      27
            
 
BanI G'GyrC_C

Cuts at:      0     144     271     890    1287    1435    1716 
   Size:        144     127     619     397     148     281

  Fragments arranged by size: 

                 619     397     281     148     144     127
 
BanII G_rGCy'C

Cuts at:      0     156     764     852    1041    1049    1349    1529    1710 
   Size:        156     608      88     189       8     300     180     181

Cuts at:   1710    1716 
   Size:          6

  Fragments arranged by size: 

                 608     300     189     181     180     156      88       8
                   6
 
BbeI G_GCGC'C

Cuts at:      0    1291    1716 
   Size:       1291     425
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     212    1716 
   Size:        212    1504
 
BbvCI CC'TCA_GC

Cuts at:      0    1366    1716 
   Size:       1366     350
 
BglI GCCn_nnn'nGGC

Cuts at:      0     179     759    1716 
   Size:        179     580     957

  Fragments arranged by size: 

                 957     580     179
 
BglII A'GATC_T

Cuts at:      0     335    1716 
   Size:        335    1381
 
Bme1580I G_kGCm'C

Cuts at:      0     156     521     580     764     893     998    1041    1049 
   Size:        156     365      59     184     129     105      43       8

Cuts at:   1049    1438    1716 
   Size:        389     278

  Fragments arranged by size: 

                 389     365     278     184     156     129     105      59
                  43       8
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     462    1455    1716 
   Size:        462     993     261

  Fragments arranged by size: 

                 993     462     261
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     767     785     866    1096    1240    1300    1342 
   Size:        346     421      18      81     230     144      60      42

Cuts at:   1342    1450    1716 
   Size:        108     266

  Fragments arranged by size: 

                 421     346     266     230     144     108      81      60
                  42      18
 
Bpu10I CC'TnA_GC

Cuts at:      0    1366    1538    1716 
   Size:       1366     172     178

  Fragments arranged by size: 

                1366     178     172
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      45    1716 
   Size:         45    1671
 
BsaAI yAC'GTr

Cuts at:      0    1677    1716 
   Size:       1677      39
 
BsaHI Gr'CG_yC

Cuts at:      0    1288    1716 
   Size:       1288     428
 
BsaWI w'CCGG_w

Cuts at:      0     360    1716 
   Size:        360    1356
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     343     346     347     379     382    1084    1716 
   Size:        343       3       1      32       3     702     632

  Fragments arranged by size: 

                 702     632     343      32       3       3       1
 
BseYI C'CCAG_C

Cuts at:      0     155     235     617     677     683     725     749     765 
   Size:        155      80     382      60       6      42      24      16

Cuts at:    765     934     950    1088    1716 
   Size:        169      16     138     628

  Fragments arranged by size: 

                 628     382     169     155     138      80      60      42
                  24      16      16       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     994    1716 
   Size:        994     722
 
BsiEI CG_ry'CG

Cuts at:      0     299    1155    1716 
   Size:        299     856     561

  Fragments arranged by size: 

                 856     561     299
 
BsiHKAI G_wGCw'C

Cuts at:      0     264    1513    1682    1716 
   Size:        264    1249     169      34

  Fragments arranged by size: 

                1249     264     169      34
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      23    1716 
   Size:         23    1693
 
Bsp1286I G_dGCh'C

Cuts at:      0     156     264     521     580     764     852     893     998 
   Size:        156     108     257      59     184      88      41     105

Cuts at:    998    1041    1049    1349    1438    1513    1529    1682    1710 
   Size:         43       8     300      89      75      16     153      28

Cuts at:   1710    1716 
   Size:          6

  Fragments arranged by size: 

                 300     257     184     156     153     108     105      89
                  88      75      59      43      41      28      16       8
                   6
 
BspEI T'CCGG_A

Cuts at:      0     360    1716 
   Size:        360    1356
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1240    1345    1716 
   Size:       1240     105     371

  Fragments arranged by size: 

                1240     371     105
 
BsrFI r'CCGG_y

Cuts at:      0    1002    1463    1716 
   Size:       1002     461     253

  Fragments arranged by size: 

                1002     461     253
 
BssHII G'CGCG_C

Cuts at:      0     159    1716 
   Size:        159    1557
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     259     993    1098    1716 
   Size:        259     734     105     618

  Fragments arranged by size: 

                 734     618     259     105
 
BstYI r'GATC_y

Cuts at:      0     335     859    1716 
   Size:        335     524     857

  Fragments arranged by size: 

                 857     524     335
 
Bsu36I CC'TnA_GG

Cuts at:      0     380     695    1716 
   Size:        380     315    1021

  Fragments arranged by size: 

                1021     380     315
 
BtgI C'CryG_G

Cuts at:      0    1373    1716 
   Size:       1373     343
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1203    1716 
   Size:       1203     513
 
BtsI GCAGTG_nn'

Cuts at:      0     457    1395    1697    1716 
   Size:        457     938     302      19

  Fragments arranged by size: 

                 938     457     302      19
 
DraIII CAC_nnn'GTG

Cuts at:      0     993    1716 
   Size:        993     723
 
EaeI y'GGCC_r

Cuts at:      0     530    1152    1545    1716 
   Size:        530     622     393     171

  Fragments arranged by size: 

                 622     530     393     171
 
EagI C'GGCC_G

Cuts at:      0    1152    1716 
   Size:       1152     564
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     767     785     866    1078    1096    1240    1300 
   Size:        346     421      18      81     212      18     144      60

Cuts at:   1300    1342    1450    1716 
   Size:         42     108     266

  Fragments arranged by size: 

                 421     346     266     212     144     108      81      60
                  42      18      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0     152     376     384     760     761    1037    1038    1045 
   Size:        152     224       8     376       1     276       1       7

Cuts at:   1045    1046    1716 
   Size:          1     670

  Fragments arranged by size: 

                 670     376     276     224     152       8       7       1
                   1       1
 
FspI TGC'GCA

Cuts at:      0    1247    1716 
   Size:       1247     469
 
HaeII r_GCGC'y

Cuts at:      0    1291    1716 
   Size:       1291     425
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     432     436     464     468    1716 
   Size:        432       4      28       4    1248

  Fragments arranged by size: 

                1248     432      28       4       4
 
HincII GTy'rAC

Cuts at:      0    1140    1716 
   Size:       1140     576
 
KasI G'GCGC_C

Cuts at:      0    1287    1716 
   Size:       1287     429
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     726    1666    1716 
   Size:        726     940      50

  Fragments arranged by size: 

                 940     726      50
 
MscI TGG'CCA

Cuts at:      0     532    1716 
   Size:        532    1184
 
MslI CAynn'nnrTG

Cuts at:      0    1241    1408    1716 
   Size:       1241     167     308

  Fragments arranged by size: 

                1241     308     167
 
MspA1I CmG'CkG

Cuts at:      0    1083    1209    1716 
   Size:       1083     126     507

  Fragments arranged by size: 

                1083     507     126
 
NarI GG'CG_CC

Cuts at:      0    1288    1716 
   Size:       1288     428
 
NspI r_CATG'y

Cuts at:      0    1365    1716 
   Size:       1365     351
 
PasI CC'CwG_GG

Cuts at:      0     410    1033    1042    1716 
   Size:        410     623       9     674

  Fragments arranged by size: 

                 674     623     410       9
 
PfoI T'CCnGG_A

Cuts at:      0     843    1716 
   Size:        843     873
 
PmlI CAC'GTG

Cuts at:      0    1677    1716 
   Size:       1677      39
 
PspOMI G'GGCC_C

Cuts at:      0     152     760    1037    1045    1716 
   Size:        152     608     277       8     671

  Fragments arranged by size: 

                 671     608     277     152       8
 
PstI C_TGCA'G

Cuts at:      0     570    1716 
   Size:        570    1146
 
PvuI CG_AT'CG

Cuts at:      0     299    1716 
   Size:        299    1417
 
PvuII CAG'CTG

Cuts at:      0    1083    1209    1716 
   Size:       1083     126     507

  Fragments arranged by size: 

                1083     507     126
 
SexAI A'CCwGG_T

Cuts at:      0    1019    1716 
   Size:       1019     697
 
SfcI C'TryA_G

Cuts at:      0     566    1272    1716 
   Size:        566     706     444

  Fragments arranged by size: 

                 706     566     444
 
SfoI GGC'GCC

Cuts at:      0    1289    1716 
   Size:       1289     427
 
SmaI CCC'GGG

Cuts at:      0     123     150     499    1177    1716 
   Size:        123      27     349     678     539

  Fragments arranged by size: 

                 678     539     349     123      27
 
SphI G_CATG'C

Cuts at:      0    1365    1716 
   Size:       1365     351
 
SrfI GCCC'GGGC

Cuts at:      0    1177    1716 
   Size:       1177     539
 
StuI AGG'CCT

Cuts at:      0    1384    1716 
   Size:       1384     332
 
StyI C'CwwG_G

Cuts at:      0     595    1716 
   Size:        595    1121
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      27     763    1716 
   Size:         27     736     953

  Fragments arranged by size: 

                 953     736      27
 
XmaI C'CCGG_G

Cuts at:      0     121     148     497    1175    1716 
   Size:        121      27     349     678     541

  Fragments arranged by size: 

                 678     541     349     121      27
 
XmnI GAAnn'nnTTC

Cuts at:      0      73     112     209     308    1716 
   Size:         73      39      97      99    1408

  Fragments arranged by size: 

                1408      99      97      73      39
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     AleI    AlwNI     ApaI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BglI    BglII Bme1580I     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrFI
   BssHII   BstAPI    BstYI   Bsu36I     BtgI    BtgZI     BtsI   DraIII
     EaeI     EagI  Eco57MI EcoO109I     FspI    HaeII    Hin4I   HincII
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NspI     PasI
     PfoI     PmlI   PspOMI     PstI     PvuI    PvuII    SexAI     SfcI
     SfoI     SmaI     SphI     SrfI     StuI     StyI    TaqII     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI
     BmrI     BmtI    BpuEI    BsaBI    BsaXI    BsiWI     BsmI    BspHI
    BsrBI    BsrDI    BsrGI    BssSI    BstBI   BstEII    BstXI  BstZ17I
     ClaI    CspCI     DraI     DrdI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PmeI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SspI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     ZraI
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