Restriction map for pF1KB8711
 (Linear) (Six-base) MAPSORT of: KIBB8711.seq  Check: 2777  from: 1  to: 1446



KIBB8711.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     974    1112    1446 
   Size:        974     138     334

  Fragments arranged by size: 

                 974     334     138
 
AflIII A'CryG_T

Cuts at:      0     138     521    1446 
   Size:        138     383     925

  Fragments arranged by size: 

                 925     383     138
 
ApoI r'AATT_y

Cuts at:      0     283     784    1446 
   Size:        283     501     662

  Fragments arranged by size: 

                 662     501     283
 
AseI AT'TA_AT

Cuts at:      0     901    1446 
   Size:        901     545
 
BglII A'GATC_T

Cuts at:      0    1130    1446 
   Size:       1130     316
 
Bme1580I G_kGCm'C

Cuts at:      0    1220    1446 
   Size:       1220     226
 
BmgBI CAC'GTC

Cuts at:      0     522    1446 
   Size:        522     924
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     286    1446 
   Size:        286    1160
 
BmtI G_CTAG'C

Cuts at:      0     691    1446 
   Size:        691     755
 
Bpu10I CC'TnA_GC

Cuts at:      0    1011    1263    1334    1446 
   Size:       1011     252      71     112

  Fragments arranged by size: 

                1011     252     112      71
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     245    1446 
   Size:         32     213    1201

  Fragments arranged by size: 

                1201     213      32
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     512    1446 
   Size:        512     934
 
BseYI C'CCAG_C

Cuts at:      0     368    1446 
   Size:        368    1078
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     501     904    1446 
   Size:        501     403     542

  Fragments arranged by size: 

                 542     501     403
 
BsiHKAI G_wGCw'C

Cuts at:      0     504    1446 
   Size:        504     942
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      85    1187    1446 
   Size:         85    1102     259

  Fragments arranged by size: 

                1102     259      85
 
Bsp1286I G_dGCh'C

Cuts at:      0     504    1220    1446 
   Size:        504     716     226

  Fragments arranged by size: 

                 716     504     226
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1121    1338    1446 
   Size:       1121     217     108

  Fragments arranged by size: 

                1121     217     108
 
BstYI r'GATC_y

Cuts at:      0     645    1130    1446 
   Size:        645     485     316

  Fragments arranged by size: 

                 645     485     316
 
ClaI AT'CG_AT

Cuts at:      0      39    1446 
   Size:         39    1407
 
DraI TTT'AAA

Cuts at:      0     797    1446 
   Size:        797     649
 
EaeI y'GGCC_r

Cuts at:      0    1197    1446 
   Size:       1197     249
 
EarI CTCTTCn'nnn_

Cuts at:      0     833    1148    1375    1446 
   Size:        833     315     227      71

  Fragments arranged by size: 

                 833     315     227      71
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     974    1112    1446 
   Size:        974     138     334

  Fragments arranged by size: 

                 974     334     138
 
EcoO109I rG'GnC_Cy

Cuts at:      0     351    1015    1079    1446 
   Size:        351     664      64     367

  Fragments arranged by size: 

                 664     367     351      64
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     120     610     664    1446 
   Size:        120     490      54     782

  Fragments arranged by size: 

                 782     490     120      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     189     221     656     688     976    1008    1027    1059 
   Size:        189      32     435      32     288      32      19      32

Cuts at:   1059    1375    1407    1446 
   Size:        316      32      39

  Fragments arranged by size: 

                 435     316     288     189      39      32      32      32
                  32      32      19
 
MfeI C'AATT_G

Cuts at:      0     224    1446 
   Size:        224    1222
 
MslI CAynn'nnrTG

Cuts at:      0     507    1446 
   Size:        507     939
 
MspA1I CmG'CkG

Cuts at:      0    1183    1446 
   Size:       1183     263
 
NdeI CA'TA_TG

Cuts at:      0     780    1446 
   Size:        780     666
 
NheI G'CTAG_C

Cuts at:      0     687    1446 
   Size:        687     759
 
NspI r_CATG'y

Cuts at:      0     142     687    1010    1446 
   Size:        142     545     323     436

  Fragments arranged by size: 

                 545     436     323     142
 
PasI CC'CwG_GG

Cuts at:      0      23    1230    1446 
   Size:         23    1207     216

  Fragments arranged by size: 

                1207     216      23
 
PciI A'CATG_T

Cuts at:      0     138    1446 
   Size:        138    1308
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     417    1446 
   Size:        417    1029
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1147    1179    1446 
   Size:       1147      32     267

  Fragments arranged by size: 

                1147     267      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1015    1079    1446 
   Size:       1015      64     367

  Fragments arranged by size: 

                1015     367      64
 
SanDI GG'GwC_CC

Cuts at:      0    1079    1446 
   Size:       1079     367
 
SmlI C'TyrA_G

Cuts at:      0      47     260    1446 
   Size:         47     213    1186

  Fragments arranged by size: 

                1186     213      47
 
SphI G_CATG'C

Cuts at:      0    1010    1446 
   Size:       1010     436
 
SspI AAT'ATT

Cuts at:      0      85    1446 
   Size:         85    1361
 
StuI AGG'CCT

Cuts at:      0     103    1315    1446 
   Size:        103    1212     131

  Fragments arranged by size: 

                1212     131     103
 
StyI C'CwwG_G

Cuts at:      0     297    1148    1247    1446 
   Size:        297     851      99     199

  Fragments arranged by size: 

                 851     297     199      99
 
TatI w'GTAC_w

Cuts at:      0     228     868    1446 
   Size:        228     640     578

  Fragments arranged by size: 

                 640     578     228
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1227    1446 
   Size:       1227     219
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AseI    BglII Bme1580I    BmgBI     BmrI
     BmtI   Bpu10I    BpuEI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BstYI     ClaI     DraI     EaeI     EarI  Eco57MI
 EcoO109I     FalI    Hin4I     MfeI     MslI   MspA1I     NdeI     NheI
     NspI     PasI     PciI    PflMI     PpiI    PpuMI    SanDI     SmlI
     SphI     SspI     StuI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     NaeI     NarI     NcoI   NgoMIV     NotI     NruI     NsiI
     PacI     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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