Restriction map for pF1KB8151
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcyI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        Asp718I     AspEI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BlnI        BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       Bsp1407I    BspDI       BspEI       
BspHI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstXI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRV       EcoT22I     
FbaI        FseI        FspI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     XbaI        
XcmI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AclI          336  AACGTT               AA^CGTT
AcsI          304  GAATTC               [GA]^AATT[CT]
AfaI           39  GTAC                 GT^AC
AflIII        867  ACATGT               A^C[GA][CT]GT
AlwNI         631  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          304  GAATTC               [GA]^AATT[CT]
AseI          805  ATTAAT               AT^TAAT
AsnI          805  ATTAAT               AT^TAAT
Asp700I       445  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI          210  GGTGCC               G^G[CT][GA]CC
BglII         447  AGATCT               A^GATCT
BlpI          144  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      144  GCTCAGC              GC^T[ACGT]AGC
BsmI          510  GAATGC               GAATGC(1/-1)
BspLU11I      867  ACATGT               A^CATGT
BspMI         573  ACCTGC               ACCTGC(4/8)
BsrDI         287  CATTGC               GCAATG(2/0)
BsrFI         207  GCCGGT               [GA]^CCGG[CT]
BstEII        607  GGTAACC              G^GT[ACGT]ACC
BstF5I        539  GGATG                GGATG(2/0)
BstPI         607  GGTAACC              G^GT[ACGT]ACC
CelII         144  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        207  GCCGGT               [GA]^CCGG[CT]
DraII         655  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EarI          496  GAAGAG               CTCTTC(1/4)
EcoO109I      655  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       607  GGTAACC              G^GT[ACGT]ACC
EcoRI         304  GAATTC               G^AATTC
EcoT14I       764  CCTTGG               C^C[AT][AT]GG
FokI          539  GGATG                GGATG(9/13)
HaeII          72  GGCGCT               [GA]GCGC^[CT]
HincII        676  GTCAAC               GT[CT]^[GA]AC
HindII        676  GTCAAC               GT[CT]^[GA]AC
Ksp632I       496  GAAGAG               CTCTTC(1/4)
NlaIII        868  CATG                 CATG^
NspI          867  ACATGT               [GA]CATG^[CT]
PaeR7I        117  CTCGAG               C^TCGAG
PflMI         634  CCACTGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         805  ATTAAT               AT^TAAT
Psp1406I      336  AACGTT               AA^CGTT
PstI           79  CTGCAG               CTGCA^G
RsaI           39  GTAC                 GT^AC
SapI          496  GAAGAGC              GCTCTTC(1/4)
SfcI           79  CTGCAG               C^T[GA][CT]AG
SgrAI         206  CGCCGGTG             C[GA]^CCGG[CT]G
StyI          764  CCTTGG               C^C[AT][AT]GG
Tsp45I        851  GTCAC                ^GT[GC]AC
Tsp509I       305  AATT                 ^AATT
Van91I        634  CCACTGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          117  CTCGAG               C^TCGAG
XmnI          445  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AluI           34  AGCT                 AG^CT
              785  AGCT                
AlwI          359  GATCC                GGATC(4/5)
              882  GGATC               
ApaI          655  GGGCCC               GGGCC^C
              706  GGGCCC              
AspHI         120  GAGCAC               G[AT]GC[AT]^C
              142  GTGCTC              
BpmI          252  CTCCAG               CTGGAG(16/14)
              697  CTCCAG              
BseRI          22  GAGGAG               GAGGAG(10/8)
              394  GAGGAG              
BsiHKAI       120  GAGCAC               G[AT]GC[AT]^C
              142  GTGCTC              
BsmBI         150  CGTCTC               CGTCTC(1/5)
              168  CGTCTC              
BsrBI         685  CCGCTC               CCGCTC(-3/-3)
               25  GAGCGG              
BstYI         358  AGATCC               [GA]^GATC[CT]
              447  AGATCT              
DraIII         94  CACGGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              635  CACTGGGTG           
HphI          243  GGTGA                GGTGA(8/7)
              781  GGTGA               
MaeII         337  ACGT                 A^CGT
              557  ACGT                
MflI          358  AGATCC               [GA]^GATC[CT]
              447  AGATCT              
MspA1I         88  CAGCGG               C[AC]G^C[GT]G
              192  CAGCGG              
SmaI          130  CCCGGG               CCC^GGG
              238  CCCGGG              
SmlI          117  CTCGAG               C^T[CT][GA]AG
              474  CTTGAG              
XhoII         358  AGATCC               [GA]^GATC[CT]
              447  AGATCT              
XmaCI         130  CCCGGG               C^CCGGG
              238  CCCGGG              
XmaI          130  CCCGGG               C^CCGGG
              238  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AccII       AciI        AvaI        BanII       BbvI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EcoRII      FauI        
Fnu4HI      HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       ItaI        MaeIII      
MboI        MboII       MnlI        MseI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIV       PleI        Sau3AI      Sau96I      ScrFI       
TaqI        TfiI        Tru9I       TseI        TspRI       
TthHB8I     
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