Restriction map for
pF1KB6664
(Linear) (Six-base) MAPSORT of: KIBB6664.seq Check: 602 from: 1 to: 1356
KIBB6664.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AclI AA'CG_TT
Cuts at: 0 185 1356
Size: 185 1171
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 1356
Size: 458 898
AflII C'TTAA_G
Cuts at: 0 163 1356
Size: 163 1193
AleI CACnn'nnGTG
Cuts at: 0 874 1356
Size: 874 482
AlwNI CAG_nnn'CTG
Cuts at: 0 508 765 1216 1356
Size: 508 257 451 140
Fragments arranged by size:
508 451 257 140
ApoI r'AATT_y
Cuts at: 0 72 360 540 635 1356
Size: 72 288 180 95 721
Fragments arranged by size:
721 288 180 95 72
AseI AT'TA_AT
Cuts at: 0 128 1356
Size: 128 1228
AvaI C'yCGr_G
Cuts at: 0 27 1356
Size: 27 1329
AvrII C'CTAG_G
Cuts at: 0 567 1356
Size: 567 789
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 809 842 1356
Size: 809 33 514
Fragments arranged by size:
809 514 33
BanI G'GyrC_C
Cuts at: 0 37 1356
Size: 37 1319
BanII G_rGCy'C
Cuts at: 0 7 1123 1356
Size: 7 1116 233
Fragments arranged by size:
1116 233 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1145 1356
Size: 1145 211
BglII A'GATC_T
Cuts at: 0 441 1356
Size: 441 915
Bme1580I G_kGCm'C
Cuts at: 0 40 1356
Size: 40 1316
BmrI ACTGGGnnnn_n'
Cuts at: 0 1294 1356
Size: 1294 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 1356
Size: 367 989
Bpu10I CC'TnA_GC
Cuts at: 0 1137 1356
Size: 1137 219
BsaI GGTCTCn'nnnn_
Cuts at: 0 235 1356
Size: 235 1121
BsaBI GATnn'nnATC
Cuts at: 0 208 1356
Size: 208 1148
BsaHI Gr'CG_yC
Cuts at: 0 1243 1356
Size: 1243 113
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1204 1234 1356
Size: 1204 30 122
Fragments arranged by size:
1204 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1212 1356
Size: 1212 144
BseYI C'CCAG_C
Cuts at: 0 966 1356
Size: 966 390
BsiHKAI G_wGCw'C
Cuts at: 0 1123 1356
Size: 1123 233
BsmI GAATG_Cn'
Cuts at: 0 296 1356
Size: 296 1060
Bsp1286I G_dGCh'C
Cuts at: 0 7 40 1123 1356
Size: 7 33 1083 233
Fragments arranged by size:
1083 233 33 7
BspHI T'CATG_A
Cuts at: 0 425 1356
Size: 425 931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 370 1356
Size: 370 986
BsrDI GCAATG_nn'
Cuts at: 0 1061 1356
Size: 1061 295
BsrGI T'GTAC_A
Cuts at: 0 494 1356
Size: 494 862
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1216 1356
Size: 1216 140
BstYI r'GATC_y
Cuts at: 0 23 168 441 1356
Size: 23 145 273 915
Fragments arranged by size:
915 273 145 23
BtsI GCAGTG_nn'
Cuts at: 0 1250 1356
Size: 1250 106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1254 1289 1356
Size: 1254 35 67
Fragments arranged by size:
1254 67 35
DraIII CAC_nnn'GTG
Cuts at: 0 397 1356
Size: 397 959
EaeI y'GGCC_r
Cuts at: 0 273 1356
Size: 273 1083
EarI CTCTTCn'nnn_
Cuts at: 0 647 1034 1128 1356
Size: 647 387 94 228
Fragments arranged by size:
647 387 228 94
EcoICRI GAG'CTC
Cuts at: 0 1121 1356
Size: 1121 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 458 1356
Size: 367 91 898
Fragments arranged by size:
898 367 91
EcoO109I rG'GnC_Cy
Cuts at: 0 1260 1356
Size: 1260 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 1135 1167 1204 1236 1356
Size: 340 32 763 32 37 32 120
Fragments arranged by size:
763 340 120 37 32 32 32
MslI CAynn'nnrTG
Cuts at: 0 874 1356
Size: 874 482
MspA1I CmG'CkG
Cuts at: 0 705 799 1356
Size: 705 94 557
Fragments arranged by size:
705 557 94
NspI r_CATG'y
Cuts at: 0 343 1356
Size: 343 1013
PflMI CCAn_nnn'nTGG
Cuts at: 0 991 1356
Size: 991 365
PfoI T'CCnGG_A
Cuts at: 0 645 1356
Size: 645 711
PshAI GACnn'nnGTC
Cuts at: 0 247 1356
Size: 247 1109
PstI C_TGCA'G
Cuts at: 0 310 372 1131 1356
Size: 310 62 759 225
Fragments arranged by size:
759 310 225 62
PvuII CAG'CTG
Cuts at: 0 705 799 1356
Size: 705 94 557
Fragments arranged by size:
705 557 94
SacI G_AGCT'C
Cuts at: 0 1123 1356
Size: 1123 233
SapI GCTCTTCn'nnn_
Cuts at: 0 1128 1356
Size: 1128 228
SexAI A'CCwGG_T
Cuts at: 0 906 1356
Size: 906 450
SfcI C'TryA_G
Cuts at: 0 213 306 368 746 1127 1356
Size: 213 93 62 378 381 229
Fragments arranged by size:
381 378 229 213 93 62
SmaI CCC'GGG
Cuts at: 0 29 1356
Size: 29 1327
SmlI C'TyrA_G
Cuts at: 0 163 1356
Size: 163 1193
StyI C'CwwG_G
Cuts at: 0 567 1106 1256 1356
Size: 567 539 150 100
Fragments arranged by size:
567 539 150 100
TatI w'GTAC_w
Cuts at: 0 494 817 1356
Size: 494 323 539
Fragments arranged by size:
539 494 323
Tth111I GACn'n_nGTC
Cuts at: 0 1282 1356
Size: 1282 74
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 263 545 1356
Size: 263 282 811
Fragments arranged by size:
811 282 263
XmaI C'CCGG_G
Cuts at: 0 27 1356
Size: 27 1329
Enzymes that do cut:
AclI AcuI AflII AleI AlwNI ApoI AseI AvaI
AvrII BaeI BanI BanII BbsI BglII Bme1580I BmrI
BpmI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI
BstYI BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI
EcoO109I Hin4I MslI MspA1I NspI PflMI PfoI PshAI
PstI PvuII SacI SapI SexAI SfcI SmaI SmlI
StyI TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmtI BplI BpuEI BsaAI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI DraI DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI XbaI XhoI XmnI ZraI