Restriction map for pF1KB5142
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       AscI        Asp700I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BbrPI       BbsI        
BbvCI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BpmI        BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspEI       
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstEII      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cfr10I      CpoI        DraI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRII      
EcoRV       FauI        FbaI        FseI        FspI        
HapII       HindIII     HpaI        HpaII       HphI        
KspI        MaeI        MamI        MluI        MluNI       
MroI        MscI        MspI        MvaI        MvnI        
NaeI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfcI        SfiI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SwaI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I        935  GGTACC               G^GTACC
AciI           18  CCGC                 CCGC(-3/-1)
AcsI          283  AAATTT               [GA]^AATT[CT]
AflIII        260  ACATGT               A^C[GA][CT]GT
AlwI          232  GGATC                GGATC(4/5)
ApaI          825  GGGCCC               GGGCC^C
ApoI          283  AAATTT               [GA]^AATT[CT]
AseI          562  ATTAAT               AT^TAAT
AsnI          562  ATTAAT               AT^TAAT
Asp718I       935  GGTACC               G^GTACC
AspHI         185  GAGCAC               G[AT]GC[AT]^C
BanII         825  GGGCCC               G[GA]GC[CT]^C
BbeI          890  GGCGCC               GGCGC^C
BlpI          979  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      979  GCTCAGC              GC^T[ACGT]AGC
BsgI          411  CTGCAC               GTGCAG(16/14)
BsiHKAI       185  GAGCAC               G[AT]GC[AT]^C
BspDI        1276  ATCGAT               AT^CGAT
BspHI         237  TCATGA               T^CATGA
BspLU11I      260  ACATGT               A^CATGT
BstBI        1215  TTCGAA               TT^CGAA
BstYI         232  GGATCT               [GA]^GATC[CT]
Cac8I         386  GCATGC               GC[ACGT]^[ACGT]GC
CelII         979  GCTCAGC              GC^T[ACGT]AGC
ClaI         1276  ATCGAT               AT^CGAT
DpnII         233  GATC                 ^GATC
DraII         825  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        680  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1245  GACTGTAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          821  CCATGG               C^C[GA][CT]GG
EcoO109I      825  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       970  ATGCAT               ATGCA^T
HgaI          639  GCGTC                GACGC(5/10)
KasI          890  GGCGCC               G^GCGCC
KpnI          935  GGTACC               GGTAC^C
MboI          233  GATC                 ^GATC
MfeI          704  CAATTG               C^AATTG
MflI          232  GGATCT               [GA]^GATC[CT]
MslI         1200  CATTGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1056  CAGCTG               C[AC]G^C[GT]G
MunI          704  CAATTG               C^AATTG
NarI          890  GGCGCC               GG^CGCC
NcoI          821  CCATGG               C^CATGG
NdeII         233  GATC                 ^GATC
NsiI          970  ATGCAT               ATGCA^T
NspV         1215  TTCGAA               TT^CGAA
PshBI         562  ATTAAT               AT^TAAT
PvuII        1056  CAGCTG               CAG^CTG
RcaI          237  TCATGA               T^CATGA
Sau3AI        233  GATC                 ^GATC
SfoI          890  GGCGCC               GGC^GCC
SfuI         1215  TTCGAA               TT^CGAA
SphI          386  GCATGC               GCATG^C
SspI          164  AATATT               AAT^ATT
StuI         1070  AGGCCT               AGG^CCT
XcmI         1129  CCATTTATAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         232  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcyI          638  GGCGTC               G[GA]^CG[CT]C
              890  GGCGCC              
BanI          890  GGCGCC               G^G[CT][GA]CC
              935  GGTACC              
BcgI          245  CGAATAAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1278  CGATATTGATGC        
BciVI         153  GTATCC               GTATCC(6/5)
              517  GTATCC              
BmyI          185  GAGCAC               G[AGT]GC[ACT]^C
              825  GGGCCC              
BsaHI         638  GGCGTC               G[GA]^CG[CT]C
              890  GGCGCC              
BsaJI          68  CCAAGG               C^C[ACGT][ACGT]GG
              821  CCATGG              
Bsp1286I      185  GAGCAC               G[AGT]GC[ACT]^C
              825  GGGCCC              
BsrDI          86  GCAATG               GCAATG(2/0)
              967  GCAATG              
CfoI          133  GCGC                 GCG^C
              891  GCGC                
Cfr13I        825  GGGCC                G^G[ACGT]CC
              826  GGCCC               
EcoT14I        68  CCAAGG               C^C[AT][AT]GG
              821  CCATGG              
HaeII         132  GGCGCT               [GA]GCGC^[CT]
              890  GGCGCC              
HhaI          133  GCGC                 GCG^C
              891  GCGC                
Hin1I         638  GGCGTC               G[GA]^CG[CT]C
              890  GGCGCC              
HinP1I        133  GCGC                 G^CGC
              891  GCGC                
MaeII         122  ACGT                 A^CGT
              151  ACGT                
MwoI          135  GCTTAATCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              377  GCTATCGAGGC         
Sau96I        825  GGGCC                G^G[ACGT]CC
              826  GGCCC               
StyI           68  CCAAGG               C^C[AT][AT]GG
              821  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AfaI        AluI        BbvI        BseRI       BsmAI       
BsrI        BstF5I      DdeI        EarI        Fnu4HI      
FokI        HaeIII      HincII      HindII      HinfI       
ItaI        Ksp632I     MaeIII      MboII       MnlI        
MseI        NlaIII      NlaIV       NspI        RsaI        
SfaNI       TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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