Restriction map for pF1KB5142
 (Linear) (Six-base) MAPSORT of: KIBB5142.seq  Check: 4048  from: 1  to: 1293



KIBB5142.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

Acc65I G'GTAC_C

Cuts at:      0     935    1293 
   Size:        935     358
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     815     863    1293 
   Size:        815      48     430

  Fragments arranged by size: 

                 815     430      48
 
AflIII A'CryG_T

Cuts at:      0     260    1293 
   Size:        260    1033
 
ApaI G_GGCC'C

Cuts at:      0     829    1293 
   Size:        829     464
 
ApoI r'AATT_y

Cuts at:      0     283    1293 
   Size:        283    1010
 
AseI AT'TA_AT

Cuts at:      0     563    1293 
   Size:        563     730
 
BanI G'GyrC_C

Cuts at:      0     890     935    1293 
   Size:        890      45     358

  Fragments arranged by size: 

                 890     358      45
 
BanII G_rGCy'C

Cuts at:      0     829    1293 
   Size:        829     464
 
BbeI G_GCGC'C

Cuts at:      0     894    1293 
   Size:        894     399
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     234     268    1267    1293 
   Size:        234      34     999      26

  Fragments arranged by size: 

                 999     234      34      26
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     164     528    1293 
   Size:        164     364     765

  Fragments arranged by size: 

                 765     364     164
 
BfrBI ATG'CAT

Cuts at:      0     972    1293 
   Size:        972     321
 
BlpI GC'TnA_GC

Cuts at:      0     980    1293 
   Size:        980     313
 
Bme1580I G_kGCm'C

Cuts at:      0     829    1293 
   Size:        829     464
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     189     615    1293 
   Size:        189     426     678

  Fragments arranged by size: 

                 678     426     189
 
BsaHI Gr'CG_yC

Cuts at:      0     639     891    1293 
   Size:        639     252     402

  Fragments arranged by size: 

                 639     402     252
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     560     607     724    1293 
   Size:        560      47     117     569

  Fragments arranged by size: 

                 569     560     117      47
 
BseYI C'CCAG_C

Cuts at:      0     180    1293 
   Size:        180    1113
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     396    1293 
   Size:        396     897
 
BsiHKAI G_wGCw'C

Cuts at:      0     189    1293 
   Size:        189    1104
 
Bsp1286I G_dGCh'C

Cuts at:      0     189     829    1293 
   Size:        189     640     464

  Fragments arranged by size: 

                 640     464     189
 
BspHI T'CATG_A

Cuts at:      0     237    1293 
   Size:        237    1056
 
BsrDI GCAATG_nn'

Cuts at:      0      93     974    1293 
   Size:         93     881     319

  Fragments arranged by size: 

                 881     319      93
 
BstBI TT'CG_AA

Cuts at:      0    1216    1293 
   Size:       1216      77
 
BstYI r'GATC_y

Cuts at:      0     232    1293 
   Size:        232    1061
 
BtgI C'CryG_G

Cuts at:      0     821    1293 
   Size:        821     472
 
BtsI GCAGTG_nn'

Cuts at:      0     531    1293 
   Size:        531     762
 
ClaI AT'CG_AT

Cuts at:      0    1277    1293 
   Size:       1277      16
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     989    1024    1293 
   Size:        989      35     269

  Fragments arranged by size: 

                 989     269      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     685    1293 
   Size:        685     608
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1251    1293 
   Size:       1251      42
 
EarI CTCTTCn'nnn_

Cuts at:      0     584     749    1037    1293 
   Size:        584     165     288     256

  Fragments arranged by size: 

                 584     288     256     165
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     815     863    1293 
   Size:        815      48     430

  Fragments arranged by size: 

                 815     430      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     826    1293 
   Size:        826     467
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1000    1293 
   Size:       1000     293
 
HaeII r_GCGC'y

Cuts at:      0     136     894    1293 
   Size:        136     758     399

  Fragments arranged by size: 

                 758     399     136
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      42      74    1147    1179    1293 
   Size:         42      32    1073      32     114

  Fragments arranged by size: 

                1073     114      42      32      32
 
HincII GTy'rAC

Cuts at:      0      15     111     120    1293 
   Size:         15      96       9    1173

  Fragments arranged by size: 

                1173      96      15       9
 
KasI G'GCGC_C

Cuts at:      0     890    1293 
   Size:        890     403
 
KpnI G_GTAC'C

Cuts at:      0     939    1293 
   Size:        939     354
 
MfeI C'AATT_G

Cuts at:      0     704    1293 
   Size:        704     589
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     245    1043    1293 
   Size:        172      73     798     250

  Fragments arranged by size: 

                 798     250     172      73
 
MslI CAynn'nnrTG

Cuts at:      0    1204    1293 
   Size:       1204      89
 
MspA1I CmG'CkG

Cuts at:      0    1058    1293 
   Size:       1058     235
 
NarI GG'CG_CC

Cuts at:      0     891    1293 
   Size:        891     402
 
NcoI C'CATG_G

Cuts at:      0     821    1293 
   Size:        821     472
 
NsiI A_TGCA'T

Cuts at:      0     974    1293 
   Size:        974     319
 
NspI r_CATG'y

Cuts at:      0     264     331     390    1293 
   Size:        264      67      59     903

  Fragments arranged by size: 

                 903     264      67      59
 
PciI A'CATG_T

Cuts at:      0     260    1293 
   Size:        260    1033
 
PsiI TTA'TAA

Cuts at:      0    1135    1293 
   Size:       1135     158
 
PspOMI G'GGCC_C

Cuts at:      0     825    1293 
   Size:        825     468
 
PvuII CAG'CTG

Cuts at:      0    1058    1293 
   Size:       1058     235
 
SfoI GGC'GCC

Cuts at:      0     892    1293 
   Size:        892     401
 
SphI G_CATG'C

Cuts at:      0     390    1293 
   Size:        390     903
 
SspI AAT'ATT

Cuts at:      0     166    1293 
   Size:        166    1127
 
StuI AGG'CCT

Cuts at:      0    1072    1293 
   Size:       1072     221
 
StyI C'CwwG_G

Cuts at:      0      68     821    1293 
   Size:         68     753     472

  Fragments arranged by size: 

                 753     472      68
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1136    1293 
   Size:       1136     157
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     ApaI     ApoI     AseI     BanI    BanII
     BbeI     BcgI    BciVI    BfrBI     BlpI Bme1580I     BmrI    BsaHI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrDI    BstBI
    BstYI     BtgI     BtsI     ClaI    CspCI   DraIII     DrdI     EarI
  Eco57MI EcoO109I     FalI    HaeII    Hin4I   HincII     KasI     KpnI
     MfeI     MmeI     MslI   MspA1I     NarI     NcoI     NsiI     NspI
     PciI     PsiI   PspOMI    PvuII     SfoI     SphI     SspI     StuI
     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BclI     BglI    BglII
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     DraI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII
     HpaI     MluI     MscI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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