Restriction map for pF1KE0204
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BalI        BbeI        BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaI        BsiHKAI     BsiWI       
BsmI        Bsp1407I    BspDI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      Eam1105I    EarI        Eco81I      EcoNI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        KasI        KpnI        Ksp632I     MamI        
MboII       MfeI        MluI        MluNI       MscI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmeI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tth111I     
XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccIII        290  TCCGGA               T^CCGGA
AcsI          313  GAATTC               [GA]^AATT[CT]
Aor51HI       527  AGCGCT               AGC^GCT
ApaI          483  GGGCCC               GGGCC^C
ApoI          313  GAATTC               [GA]^AATT[CT]
Asp700I       558  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI         599  GGATCC               G^GATCC
BanI          364  GGCACC               G^G[CT][GA]CC
BbrPI         106  CACGTG               CAC^GTG
BfaI          259  CTAG                 C^TAG
BpmI          400  CTGGAG               CTGGAG(16/14)
BsaWI         290  TCCGGA               [AT]^CCGG[AT]
BseAI         290  TCCGGA               T^CCGGA
BseRI          82  GAGGAG               GAGGAG(10/8)
BsgI           21  CTGCAC               GTGCAG(16/14)
BsiEI         472  CGGCCG               CG[GA][CT]^CG
BsmBI         307  GAGACG               CGTCTC(1/5)
BspEI         290  TCCGGA               T^CCGGA
BspHI         521  TCATGA               T^CATGA
BsrI          182  CCAGT                ACTGG(1/-1)
BssSI         346  CTCGTG               CACGAG(-5/-1)
BstAPI        507  GCAGGACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        579  GGTGACC              G^GT[ACGT]ACC
BstPI         579  GGTGACC              G^GT[ACGT]ACC
BstYI         599  GGATCC               [GA]^GATC[CT]
DdeI          431  CTAAG                C^T[ACGT]AG
DrdI          582  GACCAAGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          472  CGGCCG               [CT]^GGCC[GA]
EagI          472  CGGCCG               C^GGCCG
EclXI         472  CGGCCG               C^GGCCG
Eco47III      527  AGCGCT               AGC^GCT
Eco52I        472  CGGCCG               C^GGCCG
EcoO65I       579  GGTGACC              G^GT[ACGT]ACC
EcoRI         313  GAATTC               G^AATTC
EcoT14I       536  CCAAGG               C^C[AT][AT]GG
HgaI          120  GACGC                GACGC(5/10)
MaeI          259  CTAG                 C^TAG
MaeIII        580  GTGAC                ^GT[ACGT]AC
MflI          599  GGATCC               [GA]^GATC[CT]
MroI          290  TCCGGA               T^CCGGA
NotI          471  GCGGCCGC             GC^GGCCGC
PflMI         198  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         106  CACGTG               CAC^GTG
PmlI          106  CACGTG               CAC^GTG
PvuII         151  CAGCTG               CAG^CTG
RcaI          521  TCATGA               T^CATGA
ScaI          434  AGTACT               AGT^ACT
SfaNI         101  GCATC                GCATC(5/9)
SfcI          171  CTACAG               C^T[GA][CT]AG
SmaI          494  CCCGGG               CCC^GGG
StyI          536  CCAAGG               C^C[AT][AT]GG
TaqI          389  TCGA                 T^CGA
Tsp45I        580  GTGAC                ^GT[GC]AC
Tsp509I       314  AATT                 ^AATT
TthHB8I       389  TCGA                 T^CGA
Van91I        198  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         599  GGATCC               [GA]^GATC[CT]
XmaCI         494  CCCGGG               C^CCGGG
XmaI          494  CCCGGG               C^CCGGG
XmnI          558  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI          67  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              199  CAGGTCCTG           
AvaI          128  CTCGGG               C^[CT]CG[GA]G
              494  CCCGGG              
BanII         125  GGGCTC               G[GA]GC[CT]^C
              483  GGGCCC              
BcnI          494  CCCGG                CC^[GC]GG
              495  CCGGG               
BsaAI         106  CACGTG               [CT]AC^GT[GA]
              448  TACGTG              
BsmAI         265  GAGAC                GTCTC(1/5)
              307  GAGAC               
BsoBI         128  CTCGGG               C^[CT]CG[GA]G
              494  CCCGGG              
CfoI           10  GCGC                 GCG^C
              528  GCGC                
DsaI          225  CCGCGG               C^C[GA][CT]GG
              475  CCGCGG              
FauI          224  CCCGC                CCCGC(4/6)
              123  GCGGG               
HaeII           9  AGCGCC               [GA]GCGC^[CT]
              527  AGCGCT              
HhaI           10  GCGC                 GCG^C
              528  GCGC                
HinP1I         10  GCGC                 G^CGC
              528  GCGC                
HphI          579  GGTGA                GGTGA(8/7)
              458  TCACC               
KspI          225  CCGCGG               CCGC^GG
              475  CCGCGG              
MslI          102  CATCCACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              573  CACGACGGTG          
NciI          494  CCCGG                CC^[GC]GG
              495  CCGGG               
NlaIII        327  CATG                 CATG^
              522  CATG                
PpuMI         200  AGGTCCT              [GA]G^G[AT]CC[CT]
              589  AGGTCCC             
SacII         225  CCGCGG               CCGC^GG
              475  CCGCGG              
TspRI         183  CAGTG                CA[GC]TG[ACGT][ACGT]^
              219  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaII       BbvI        BmyI        BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       Cac8I       Cfr13I      DpnII       
DraII       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HpaII       ItaI        MaeII       
MboI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeII       NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       TseI        
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