Restriction map for
pF1KE0204
(Linear) (Six-base) MAPSORT of: KIEE0204.seq Check: 3823 from: 1 to: 621
KIEE0204.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 621
Size: 538 83
AfeI AGC'GCT
Cuts at: 0 529 621
Size: 529 92
AleI CACnn'nnGTG
Cuts at: 0 577 621
Size: 577 44
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 621
Size: 367 32 222
Fragments arranged by size:
367 222 32
AlwNI CAG_nnn'CTG
Cuts at: 0 72 204 621
Size: 72 132 417
Fragments arranged by size:
417 132 72
ApaI G_GGCC'C
Cuts at: 0 487 621
Size: 487 134
ApoI r'AATT_y
Cuts at: 0 313 621
Size: 313 308
AvaI C'yCGr_G
Cuts at: 0 128 494 621
Size: 128 366 127
Fragments arranged by size:
366 128 127
BamHI G'GATC_C
Cuts at: 0 599 621
Size: 599 22
BanI G'GyrC_C
Cuts at: 0 364 621
Size: 364 257
BanII G_rGCy'C
Cuts at: 0 129 487 621
Size: 129 358 134
Fragments arranged by size:
358 134 129
Bme1580I G_kGCm'C
Cuts at: 0 225 487 621
Size: 225 262 134
Fragments arranged by size:
262 225 134
BmgBI CAC'GTC
Cuts at: 0 513 621
Size: 513 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 621
Size: 421 200
BsaAI yAC'GTr
Cuts at: 0 108 450 621
Size: 108 342 171
Fragments arranged by size:
342 171 108
BsaWI w'CCGG_w
Cuts at: 0 290 621
Size: 290 331
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 367 397 621
Size: 367 30 224
Fragments arranged by size:
367 224 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 621
Size: 97 524
BseYI C'CCAG_C
Cuts at: 0 251 621
Size: 251 370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 621
Size: 6 615
BsiEI CG_ry'CG
Cuts at: 0 475 621
Size: 475 146
BsmBI CGTCTCn'nnnn_
Cuts at: 0 301 621
Size: 301 320
Bsp1286I G_dGCh'C
Cuts at: 0 129 225 487 621
Size: 129 96 262 134
Fragments arranged by size:
262 134 129 96
BspEI T'CCGG_A
Cuts at: 0 290 621
Size: 290 331
BspHI T'CATG_A
Cuts at: 0 521 621
Size: 521 100
BssSI C'ACGA_G
Cuts at: 0 346 621
Size: 346 275
BstAPI GCAn_nnn'nTGC
Cuts at: 0 513 621
Size: 513 108
BstEII G'GTnAC_C
Cuts at: 0 579 621
Size: 579 42
BstYI r'GATC_y
Cuts at: 0 599 621
Size: 599 22
BtgI C'CryG_G
Cuts at: 0 225 475 621
Size: 225 250 146
Fragments arranged by size:
250 225 146
DrdI GACnn_nn'nnGTC
Cuts at: 0 588 621
Size: 588 33
EaeI y'GGCC_r
Cuts at: 0 472 621
Size: 472 149
EagI C'GGCC_G
Cuts at: 0 472 621
Size: 472 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 6 621
Size: 6 615
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 538 621
Size: 421 117 83
Fragments arranged by size:
421 117 83
EcoO109I rG'GnC_Cy
Cuts at: 0 201 483 484 590 621
Size: 201 282 1 106 31
Fragments arranged by size:
282 201 106 31 1
EcoRI G'AATT_C
Cuts at: 0 313 621
Size: 313 308
HaeII r_GCGC'y
Cuts at: 0 13 531 621
Size: 13 518 90
Fragments arranged by size:
518 90 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 111 143 579 611 621
Size: 111 32 436 32 10
Fragments arranged by size:
436 111 32 32 10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 621
Size: 577 44
MslI CAynn'nnrTG
Cuts at: 0 106 577 621
Size: 106 471 44
Fragments arranged by size:
471 106 44
MspA1I CmG'CkG
Cuts at: 0 153 227 477 621
Size: 153 74 250 144
Fragments arranged by size:
250 153 144 74
NotI GC'GGCC_GC
Cuts at: 0 472 621
Size: 472 149
PflMI CCAn_nnn'nTGG
Cuts at: 0 204 621
Size: 204 417
PmlI CAC'GTG
Cuts at: 0 108 621
Size: 108 513
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 621
Size: 367 32 222
Fragments arranged by size:
367 222 32
PpuMI rG'GwC_Cy
Cuts at: 0 201 590 621
Size: 201 389 31
Fragments arranged by size:
389 201 31
PspOMI G'GGCC_C
Cuts at: 0 483 621
Size: 483 138
PvuII CAG'CTG
Cuts at: 0 153 621
Size: 153 468
SacII CC_GC'GG
Cuts at: 0 228 478 621
Size: 228 250 143
Fragments arranged by size:
250 228 143
ScaI AGT'ACT
Cuts at: 0 436 621
Size: 436 185
SfcI C'TryA_G
Cuts at: 0 171 621
Size: 171 450
SmaI CCC'GGG
Cuts at: 0 496 621
Size: 496 125
StyI C'CwwG_G
Cuts at: 0 536 621
Size: 536 85
TatI w'GTAC_w
Cuts at: 0 434 569 621
Size: 434 135 52
Fragments arranged by size:
434 135 52
XmaI C'CCGG_G
Cuts at: 0 494 621
Size: 494 127
XmnI GAAnn'nnTTC
Cuts at: 0 562 621
Size: 562 59
Enzymes that do cut:
AcuI AfeI AleI AloI AlwNI ApaI ApoI AvaI
BamHI BanI BanII Bme1580I BmgBI BpmI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspEI
BspHI BssSI BstAPI BstEII BstYI BtgI DrdI EaeI
EagI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I MmeI
MslI MspA1I NotI PflMI PmlI PpiI PpuMI PspOMI
PvuII SacII ScaI SfcI SmaI StyI TatI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaHI BsiHKAI BsiWI BsmI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstBI BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII EarI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI ZraI