Restriction map for pF1KB6321
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BmyI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmI        BsoBI       
Bsp1286I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrI        BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      EaeI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       FauI        FseI        FspI        
HapII       Hin1I       HincII      HindII      HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TfiI        Tru9I       Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AvaII          23  GGACC                G^G[AT]CC
BanI           94  GGCACC               G^G[CT][GA]CC
BclI          707  TGATCA               T^GATCA
BfaI          455  CTAG                 C^TAG
BlpI          739  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      739  GCTGAGC              GC^T[ACGT]AGC
BsmBI         144  GAGACG               CGTCTC(1/5)
BsmFI         383  GTCCC                GGGAC(10/14)
Bsp1407I      404  TGTACA               T^GTACA
BsrGI         404  TGTACA               T^GTACA
BssKI          20  CCAGG                ^CC[ACGT]GG
BssSI         718  CACGAG               CACGAG(-5/-1)
BstNI          20  CCAGG                CC^[AT]GG
CelII         739  GCTGAGC              GC^T[ACGT]AGC
Cfr13I         23  GGACC                G^G[ACGT]CC
DpnII         708  GATC                 ^GATC
DrdI          552  GACCTTGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII         20  CCAGG                ^CC[AT]GG
EcoRV         346  GATATC               GAT^ATC
EcoT22I       486  ATGCAT               ATGCA^T
FbaI          707  TGATCA               T^GATCA
HaeIII        480  GGCC                 GG^CC
HgaI          146  GACGC                GACGC(5/10)
HinfI          16  GACTC                G^A[ACGT]TC
MaeI          455  CTAG                 C^TAG
MaeII         381  ACGT                 A^CGT
MboI          708  GATC                 ^GATC
MspA1I        509  CAGCTG               C[AC]G^C[GT]G
MvaI           20  CCAGG                CC^[AT]GG
MwoI           70  GCCATGGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         708  GATC                 ^GATC
NsiI          486  ATGCAT               ATGCA^T
PleI           16  GACTC                GAGTC(4/5)
PstI          506  CTGCAG               CTGCA^G
PvuII         509  CAGCTG               CAG^CTG
Sau3AI        708  GATC                 ^GATC
Sau96I         23  GGACC                G^G[ACGT]CC
ScaI          585  AGTACT               AGT^ACT
ScrFI          20  CCAGG                CC^[ACGT]GG
SfaNI         244  GATGC                GCATC(5/9)
SfcI          506  CTGCAG               C^T[GA][CT]AG
SspBI         404  TGTACA               T^GTACA
TaqI            6  TCGA                 T^CGA
Tsp45I        449  GTGAC                ^GT[GC]AC
TspRI         222  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I         6  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          107  GAATTT               [GA]^AATT[CT]
              191  AAATTT              
AfaI          405  GTAC                 GT^AC
              586  GTAC                
Aor51HI       336  AGCGCT               AGC^GCT
              502  AGCGCT              
ApoI          107  GAATTT               [GA]^AATT[CT]
              191  AAATTT              
BpmI           18  CTCCAG               CTGGAG(16/14)
               38  CTCCAG              
BsaJI          71  CCATGG               C^C[ACGT][ACGT]GG
              599  CCATGG              
BsmAI         144  GAGAC                GTCTC(1/5)
                3  GTCTC               
BsrDI         276  CATTGC               GCAATG(2/0)
              398  CATTGC              
CfoI          337  GCGC                 GCG^C
              503  GCGC                
DdeI          268  CTAAG                C^T[ACGT]AG
              740  CTGAG               
DsaI           71  CCATGG               C^C[GA][CT]GG
              599  CCATGG              
Eco47III      336  AGCGCT               AGC^GCT
              502  AGCGCT              
EcoT14I        71  CCATGG               C^C[AT][AT]GG
              599  CCATGG              
HaeII         336  AGCGCT               [GA]GCGC^[CT]
              502  AGCGCT              
HhaI          337  GCGC                 GCG^C
              503  GCGC                
HinP1I        337  GCGC                 G^CGC
              503  GCGC                
HindIII       160  AAGCTT               A^AGCTT
              356  AAGCTT              
MaeIII        331  GTTAC                ^GT[ACGT]AC
              449  GTGAC               
NcoI           71  CCATGG               C^CATGG
              599  CCATGG              
NlaIV          94  GGCACC               GG[ACGT]^[ACGT]CC
              306  GGAGCC              
NspI           87  ACATGC               [GA]CATG^[CT]
              484  ACATGC              
RsaI          405  GTAC                 GT^AC
              586  GTAC                
StyI           71  CCATGG               C^C[AT][AT]GG
              599  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AluI        BbvI        BstF5I      Cac8I       Fnu4HI      
FokI        ItaI        MboII       MnlI        NlaIII      
TseI        Tsp509I     
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