Restriction map for pF1KB8864
 (Circular) (Six-base) MAPSORT of: pF1KB8864.seq  Check: 3202  from: 1  to: 3443



pF1KB8864  3443 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    282     291     282 
   Size:          9    3434
 
AccI GT'mk_AC

Cuts at:    481     481 
   Size:       3443
 
Acc65I G'GTAC_C

Cuts at:    459     459 
   Size:       3443
 
AclI AA'CG_TT

Cuts at:    437    3283     437 
   Size:       2846     597
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1268    1700    2447    2715    1268 
   Size:        432     747     268    1996

  Fragments arranged by size: 

                1996     747     432     268
 
AflIII A'CryG_T

Cuts at:    795    1899    2876     795 
   Size:       1104     977    1362

  Fragments arranged by size: 

                1362    1104     977
 
AgeI A'CCGG_T

Cuts at:   1831    1831 
   Size:       3443
 
AlwNI CAG_nnn'CTG

Cuts at:    404     748    2315     404 
   Size:        344    1567    1532

  Fragments arranged by size: 

                1567    1532     344
 
ApaI G_GGCC'C

Cuts at:    322     322 
   Size:       3443
 
ApaLI G'TGCA_C

Cuts at:   2213    2213 
   Size:       3443
 
ApoI r'AATT_y

Cuts at:    447    2796    3052     447 
   Size:       2349     256     838

  Fragments arranged by size: 

                2349     838     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3443
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3443
 
AvaI C'yCGr_G

Cuts at:    463     463 
   Size:       3443
 
BamHI G'GATC_C

Cuts at:    468     468 
   Size:       3443
 
BanI G'GyrC_C

Cuts at:    459     873    1121    1156     459 
   Size:        414     248      35    2746

  Fragments arranged by size: 

                2746     414     248      35
 
BanII G_rGCy'C

Cuts at:    322     457    1487     322 
   Size:        135    1030    2278

  Fragments arranged by size: 

                2278    1030     135
 
BbeI G_GCGC'C

Cuts at:    877    1125     877 
   Size:        248    3195
 
BciVI GTATCCnnnnn_n'

Cuts at:   1341    2102    3443    1341 
   Size:        761    1341    1341

  Fragments arranged by size: 

                1341    1341     761
 
BclI T'GATC_A

Cuts at:    963     963 
   Size:       3443
 
BglII A'GATC_T

Cuts at:    958    2995     958 
   Size:       2037    1406
 
BlpI GC'TnA_GC

Cuts at:    555     555 
   Size:       3443
 
Bme1580I G_kGCm'C

Cuts at:    322    1068    1161    2217     322 
   Size:        746      93    1056    1548

  Fragments arranged by size: 

                1548    1056     746      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    655     736    1071    3215     655 
   Size:         81     335    2144     883

  Fragments arranged by size: 

                2144     883     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1810    1810 
   Size:       3443
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    373     442     373 
   Size:         69    3374
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    619    1498    1990    2288     619 
   Size:        879     492     298    1774

  Fragments arranged by size: 

                1774     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3147    3147 
   Size:       3443
 
BsaAI yAC'GTr

Cuts at:    796     796 
   Size:       3443
 
BsaBI GATnn'nnATC

Cuts at:      6     957    2994       6 
   Size:        951    2037     455

  Fragments arranged by size: 

                2037     951     455
 
BsaHI Gr'CG_yC

Cuts at:    874    1122    1824    3320     874 
   Size:        248     702    1496     997

  Fragments arranged by size: 

                1496     997     702     248
 
BsaWI w'CCGG_w

Cuts at:    632    1153    1831    2105    2252    2663     632 
   Size:        521     678     274     147     411    1412

  Fragments arranged by size: 

                1412     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    168     309     868    1626    2203    2699     168 
   Size:        141     559     758     577     496     912

  Fragments arranged by size: 

                 912     758     577     559     496     141
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    347     347 
   Size:       3443
 
BsiEI CG_ry'CG

Cuts at:    104     985    1031    2239     104 
   Size:        881      46    1208    1308

  Fragments arranged by size: 

                1308    1208     881      46
 
BsiHKAI G_wGCw'C

Cuts at:    457    1235    1425    2217     457 
   Size:        778     190     792    1683

  Fragments arranged by size: 

                1683     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    322     457    1068    1161    1235    1425    1487    2217     322 
   Size:        135     611      93      74     190      62     730    1548

  Fragments arranged by size: 

                1548     730     611     190     135      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2663    2663 
   Size:       3443
 
BspHI T'CATG_A

Cuts at:   2619    2619 
   Size:       3443
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    282     291     296     493     707    1009    1390     282 
   Size:          9       5     197     214     302     381    2335

  Fragments arranged by size: 

                2335     381     302     214     197       9       5
 
BsrBI CCG'CTC

Cuts at:     50    1735    1789    3273    3438      50 
   Size:       1685      54    1484     165      55

  Fragments arranged by size: 

                1685    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1355    1355 
   Size:       3443
 
BsrFI r'CCGG_y

Cuts at:   1441    1622    1831    1441 
   Size:        181     209    3053

  Fragments arranged by size: 

                3053     209     181
 
BssSI C'ACGA_G

Cuts at:   1714    2072    1714 
   Size:        358    3085
 
BstAPI GCAn_nnn'nTGC

Cuts at:    820     820 
   Size:       3443
 
BstBI TT'CG_AA

Cuts at:   1804    1804 
   Size:       3443
 
BstYI r'GATC_y

Cuts at:      1     468     935     958    1293    1539    2551    2637    2649 
   Size:        467     467      23     335     246    1012      86      12

Cuts at:   2649    2995       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3443
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1393    1574    1737    1393 
   Size:        181     163    3099

  Fragments arranged by size: 

                3099     181     163
 
BtsI GCAGTG_nn'

Cuts at:    686     686 
   Size:       3443
 
DraI TTT'AAA

Cuts at:    442     442 
   Size:       3443
 
DraIII CAC_nnn'GTG

Cuts at:    213     213 
   Size:       3443
 
DrdI GACnn_nn'nnGTC

Cuts at:   1149    2007    1149 
   Size:        858    2585
 
EaeI y'GGCC_r

Cuts at:   1028    1202    1593    1620    2965    1028 
   Size:        174     391      27    1345    1506

  Fragments arranged by size: 

                1506    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1028    1028 
   Size:       3443
 
EarI CTCTTCn'nnn_

Cuts at:   1466    1676    1466 
   Size:        210    3233
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1651    1961    2107    3253    1651 
   Size:        310     146    1146    1841

  Fragments arranged by size: 

                1841    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    455     455 
   Size:       3443
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    373     442    1268    1700    2447    2715     373 
   Size:         69     826     432     747     268    1101

  Fragments arranged by size: 

                1101     826     747     432     268      69
 
EcoNI CCTnn'n_nnAGG

Cuts at:    326     326 
   Size:       3443
 
EcoO109I rG'GnC_Cy

Cuts at:    160     285     318     319     359     582     160 
   Size:        125      33       1      40     223    3021

  Fragments arranged by size: 

                3021     223     125      40      33       1
 
EcoRI G'AATT_C

Cuts at:    447     447 
   Size:       3443
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1462    1462 
   Size:       3443
 
FspI TGC'GCA

Cuts at:   1224    1224 
   Size:       3443
 
HaeII r_GCGC'y

Cuts at:    145     877    1125    2147    3126     145 
   Size:        732     248    1022     979     462

  Fragments arranged by size: 

                1022     979     732     462     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1285    1315    1317    1347    1285 
   Size:         30       2      30    3381

  Fragments arranged by size: 

                3381      30      30       2
 
HincII GTy'rAC

Cuts at:    482     482 
   Size:       3443
 
KasI G'GCGC_C

Cuts at:    873    1121     873 
   Size:        248    3195
 
KpnI G_GTAC'C

Cuts at:    463     463 
   Size:       3443
 
MluI A'CGCG_T

Cuts at:   2876    2876 
   Size:       3443
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2114    2298    2114 
   Size:        184    3259
 
MscI TGG'CCA

Cuts at:   1204    1204 
   Size:       3443
 
MslI CAynn'nnrTG

Cuts at:   1432    1559    1432 
   Size:        127    3316
 
MspA1I CmG'CkG

Cuts at:    420     554     868    1228    2241    2486     420 
   Size:        134     314     360    1013     245    1377

  Fragments arranged by size: 

                1377    1013     360     314     245     134
 
NaeI GCC'GGC

Cuts at:   1624    1624 
   Size:       3443
 
NarI GG'CG_CC

Cuts at:    874    1122     874 
   Size:        248    3195
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3443
 
NgoMIV G'CCGG_C

Cuts at:   1622    1622 
   Size:       3443
 
NspI r_CATG'y

Cuts at:    496     996    1903     496 
   Size:        500     907    2036

  Fragments arranged by size: 

                2036     907     500
 
PciI A'CATG_T

Cuts at:   1899    1899 
   Size:       3443
 
PmeI GTTT'AAAC

Cuts at:    442     442 
   Size:       3443
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2582    2614    2582 
   Size:         32    3411
 
PpuMI rG'GwC_Cy

Cuts at:    160     285     359     160 
   Size:        125      74    3244

  Fragments arranged by size: 

                3244     125      74
 
PspOMI G'GGCC_C

Cuts at:    318     318 
   Size:       3443
 
PstI C_TGCA'G

Cuts at:    293     341     368     490    1175     293 
   Size:         48      27     122     685    2561

  Fragments arranged by size: 

                2561     685     122      48      27
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3443
 
PvuII CAG'CTG

Cuts at:    420     868    1228     420 
   Size:        448     360    2635

  Fragments arranged by size: 

                2635     448     360
 
RsrII CG'GwC_CG

Cuts at:   1638    1638 
   Size:       3443
 
SacI G_AGCT'C

Cuts at:    457     457 
   Size:       3443
 
SalI G'TCGA_C

Cuts at:    480     480 
   Size:       3443
 
SanDI GG'GwC_CC

Cuts at:    359     359 
   Size:       3443
 
SapI GCTCTTCn'nnn_

Cuts at:   1466    1676    1466 
   Size:        210    3233
 
SbfI CC_TGCA'GG

Cuts at:    293     490     293 
   Size:        197    3246
 
ScaI AGT'ACT

Cuts at:   2960    2960 
   Size:       3443
 
SexAI A'CCwGG_T

Cuts at:    209     209 
   Size:       3443
 
SfcI C'TryA_G

Cuts at:     33     289     337     364     486    1171    2164    2355      33 
   Size:        256      48      27     122     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     256     191     122      48      27
            
 
SfoI GGC'GCC

Cuts at:    875    1123     875 
   Size:        248    3195
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3443
 
SmaI CCC'GGG

Cuts at:    465     465 
   Size:       3443
 
SmlI C'TyrA_G

Cuts at:    598    1513    2005    2267     598 
   Size:        915     492     262    1774

  Fragments arranged by size: 

                1774     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3443
 
SphI G_CATG'C

Cuts at:    496     996     496 
   Size:        500    2943
 
StyI C'CwwG_G

Cuts at:    107     344     577     931     107 
   Size:        237     233     354    2619

  Fragments arranged by size: 

                2619     354     237     233
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    276    1026    1159    1618    1827     276 
   Size:        750     133     459     209    1892

  Fragments arranged by size: 

                1892     750     459     209     133
 
TatI w'GTAC_w

Cuts at:   2958    2958 
   Size:       3443
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2960    2992    2960 
   Size:         32    3411
 
Tth111I GACn'n_nGTC

Cuts at:   1240    1240 
   Size:       3443
 
XbaI T'CTAG_A

Cuts at:    474     474 
   Size:       3443
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    938     938 
   Size:       3443
 
XmaI C'CCGG_G

Cuts at:    463     463 
   Size:       3443
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     AscI
    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI   Bpu10I    BsaXI    BseRI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PshAI
     PsiI    PspXI     PsrI    SacII     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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