Restriction map for pF1KB6986
 (Linear) (Six-base) MAPSORT of: KIBB6986.seq  Check: 4546  from: 1  to: 744



KIBB6986.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     686     744 
   Size:        361     325      58

  Fragments arranged by size: 

                 361     325      58
 
ApaI G_GGCC'C

Cuts at:      0     261     744 
   Size:        261     483
 
AvaI C'yCGr_G

Cuts at:      0     260     447     744 
   Size:        260     187     297

  Fragments arranged by size: 

                 297     260     187
 
AvrII C'CTAG_G

Cuts at:      0     424     744 
   Size:        424     320
 
BanI G'GyrC_C

Cuts at:      0     490     744 
   Size:        490     254
 
BanII G_rGCy'C

Cuts at:      0      88     261     744 
   Size:         88     173     483

  Fragments arranged by size: 

                 483     173      88
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      18     744 
   Size:         18     726
 
BclI T'GATC_A

Cuts at:      0     572     744 
   Size:        572     172
 
BglI GCCn_nnn'nGGC

Cuts at:      0     469     744 
   Size:        469     275
 
BglII A'GATC_T

Cuts at:      0     182     744 
   Size:        182     562
 
Bme1580I G_kGCm'C

Cuts at:      0     261     454     744 
   Size:        261     193     290

  Fragments arranged by size: 

                 290     261     193
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535     744 
   Size:        535     209
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634     744 
   Size:        634     110
 
BsaBI GATnn'nnATC

Cuts at:      0     247     744 
   Size:        247     497
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     127     157     744 
   Size:        127      30     587

  Fragments arranged by size: 

                 587     127      30
 
BsiEI CG_ry'CG

Cuts at:      0      12      92     744 
   Size:         12      80     652

  Fragments arranged by size: 

                 652      80      12
 
Bsp1286I G_dGCh'C

Cuts at:      0      88     261     454     744 
   Size:         88     173     193     290

  Fragments arranged by size: 

                 290     193     173      88
 
BspHI T'CATG_A

Cuts at:      0     629     744 
   Size:        629     115
 
BsrDI GCAATG_nn'

Cuts at:      0     369     744 
   Size:        369     375
 
BsrFI r'CCGG_y

Cuts at:      0     281     744 
   Size:        281     463
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     567     744 
   Size:        567     177
 
BstYI r'GATC_y

Cuts at:      0     182     744 
   Size:        182     562
 
BtgI C'CryG_G

Cuts at:      0      92     744 
   Size:         92     652
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     315     744 
   Size:        315     429
 
DraI TTT'AAA

Cuts at:      0     659     744 
   Size:        659      85
 
EaeI y'GGCC_r

Cuts at:      0      39     149     744 
   Size:         39     110     595

  Fragments arranged by size: 

                 595     110      39
 
EarI CTCTTCn'nnn_

Cuts at:      0      52     105     744 
   Size:         52      53     639

  Fragments arranged by size: 

                 639      53      52
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     535     686     744 
   Size:        361     174     151      58

  Fragments arranged by size: 

                 361     174     151      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0     257     297     744 
   Size:        257      40     447

  Fragments arranged by size: 

                 447     257      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     127     159     624     645     656     677     744 
   Size:        127      32     465      21      11      21      67

  Fragments arranged by size: 

                 465     127      67      32      21      21      11
 
MscI TGG'CCA

Cuts at:      0     151     744 
   Size:        151     593
 
MspA1I CmG'CkG

Cuts at:      0      94     744 
   Size:         94     650
 
PfoI T'CCnGG_A

Cuts at:      0     512     584     744 
   Size:        512      72     160

  Fragments arranged by size: 

                 512     160      72
 
PpuMI rG'GwC_Cy

Cuts at:      0     297     744 
   Size:        297     447
 
PspOMI G'GGCC_C

Cuts at:      0     257     744 
   Size:        257     487
 
PstI C_TGCA'G

Cuts at:      0     161     744 
   Size:        161     583
 
SacII CC_GC'GG

Cuts at:      0      95     744 
   Size:         95     649
 
SfcI C'TryA_G

Cuts at:      0     157     744 
   Size:        157     587
 
SmaI CCC'GGG

Cuts at:      0     262     744 
   Size:        262     482
 
SmlI C'TyrA_G

Cuts at:      0     649     744 
   Size:        649      95
 
StuI AGG'CCT

Cuts at:      0     224     744 
   Size:        224     520
 
StyI C'CwwG_G

Cuts at:      0     424     744 
   Size:        424     320
 
TatI w'GTAC_w

Cuts at:      0     314     744 
   Size:        314     430
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     171     203     744 
   Size:        171      32     541

  Fragments arranged by size: 

                 541     171      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     202     301     744 
   Size:        202      99     443

  Fragments arranged by size: 

                 443     202      99
 
XmaI C'CCGG_G

Cuts at:      0     260     744 
   Size:        260     484
 
XmnI GAAnn'nnTTC

Cuts at:      0     593     744 
   Size:        593     151
 

 Enzymes that do cut:

     AcuI     ApaI     AvaI    AvrII     BanI    BanII     BbsI     BclI
     BglI    BglII Bme1580I     BpmI    BpuEI    BsaBI    BsaXI    BsiEI
 Bsp1286I    BspHI    BsrDI    BsrFI    BstXI    BstYI     BtgI    BtgZI
     DraI     EaeI     EarI  Eco57MI EcoO109I    Hin4I     MscI   MspA1I
     PfoI    PpuMI   PspOMI     PstI    SacII     SfcI     SmaI     SmlI
     StuI     StyI     TatI     TstI  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     XbaI     XcmI     XhoI     ZraI
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