Restriction map for pF1KB5930
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# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
AocI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbvCI       BciVI       BclI        BfrI        BglI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstYI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FspI        HgaI        Hin1I       HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MunI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        StyI        SwaI        
TfiI        Tsp509I     XbaI        XhoII       XmnI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII           5  CGCG                 CG^CG
AflIII       1463  ACACGT               A^C[GA][CT]GT
Alw44I        878  GTGCAC               G^TGCAC
AlwI         1101  GGATC                GGATC(4/5)
ApaLI         878  GTGCAC               G^TGCAC
AspHI         878  GTGCAC               G[AT]GC[AT]^C
BanI          927  GGTGCC               G^G[CT][GA]CC
BcgI          426  CGAAGACCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         1079  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1079  GCTCAGC              GC^T[ACGT]AGC
BsiHKAI       878  GTGCAC               G[AT]GC[AT]^C
BsrBI         820  GAGCGG               CCGCTC(-3/-3)
BstEII       1403  GGTCACC              G^GT[ACGT]ACC
BstPI        1403  GGTCACC              G^GT[ACGT]ACC
BstUI           5  CGCG                 CG^CG
CelII        1079  GCTCAGC              GC^T[ACGT]AGC
CfoI          414  GCGC                 GCG^C
DsaI          964  CCACGG               C^C[GA][CT]GG
EarI          166  GAAGAG               CTCTTC(1/4)
EcoO65I      1403  GGTCACC              G^GT[ACGT]ACC
FseI         1446  GGCCGGCC             GGCCGG^CC
HaeII         413  GGCGCT               [GA]GCGC^[CT]
HhaI          414  GCGC                 GCG^C
HinP1I        414  GCGC                 G^CGC
HincII        286  GTCAAC               GT[CT]^[GA]AC
HindII        286  GTCAAC               GT[CT]^[GA]AC
HindIII       457  AAGCTT               A^AGCTT
Ksp632I       166  GAAGAG               CTCTTC(1/4)
MseI          688  TTAA                 T^TAA
MslI          244  CACATGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI            5  CGCG                 CG^CG
PaeR7I        579  CTCGAG               C^TCGAG
PshAI        1266  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI          166  GAAGAGC              GCTCTTC(1/4)
SmaI         1528  CCCGGG               CCC^GGG
SphI          225  GCATGC               GCATG^C
StuI         1236  AGGCCT               AGG^CCT
Tru9I         688  TTAA                 T^TAA
XcmI          896  CCAGCCTGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          579  CTCGAG               C^TCGAG
XmaCI        1528  CCCGGG               C^CCGGG
XmaI         1528  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI        1047  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1133  CAGTGACTG           
ApaI          607  GGGCCC               GGGCC^C
             1318  GGGCCC              
BsaI          491  GAGACC               GGTCTC(1/5)
             1416  GGTCTC              
Bsp1407I      839  TGTACA               T^GTACA
              947  TGTACA              
BsrGI         839  TGTACA               T^GTACA
              947  TGTACA              
BstXI         318  CCAGGAGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1159  CCATGCACCTGG        
DpnII         525  GATC                 ^GATC
             1102  GATC                
EcoNI         837  CCTGTACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1306  CCTGGGCTAGG         
FauI          600  GCGGG                CCCGC(4/6)
             1432  GCGGG               
MaeII         374  ACGT                 A^CGT
             1465  ACGT                
MboI          525  GATC                 ^GATC
             1102  GATC                
NaeI          357  GCCGGC               GCC^GGC
             1447  GCCGGC              
NdeII         525  GATC                 ^GATC
             1102  GATC                
NgoMIV        357  GCCGGC               G^CCGGC
             1447  GCCGGC              
NspI          225  GCATGC               [GA]CATG^[CT]
              443  ACATGC              
Sau3AI        525  GATC                 ^GATC
             1102  GATC                
SmlI          537  CTCAAG               C^T[CT][GA]AG
              579  CTCGAG              
SspBI         839  TGTACA               T^GTACA
              947  TGTACA              
TaqI          580  TCGA                 T^CGA
             1591  TCGA                
TthHB8I       580  TCGA                 T^CGA
             1591  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AfaI        AluI        AspI        AvaI        
AvaII       BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr10I      
Cfr13I      DdeI        DraII       EaeI        EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboII       MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
PflFI       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        Sau96I      ScrFI       SfaNI       
SfcI        TseI        Tsp45I      TspRI       Tth111I     
Van91I      
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