(Circular) (Six-base) MAPSORT of: pF1KE2764.seq Check: 2945 from: 1 to: 4685 pF1KE2764 4685 bp With 182 enzymes: SgfI * July 3, 2020 20:21 .. AccI GT'mk_AC Cuts at: 1723 1723 Size: 4685 Acc65I G'GTAC_C Cuts at: 1701 1701 Size: 4685 AclI AA'CG_TT Cuts at: 1679 4525 1679 Size: 2846 1839 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 358 2510 2942 3689 3957 358 Size: 2152 432 747 268 1086 Fragments arranged by size: 2152 1086 747 432 268 AflIII A'CryG_T Cuts at: 410 780 1193 2037 3141 4118 410 Size: 370 413 844 1104 977 977 Fragments arranged by size: 1104 977 977 844 413 370 AgeI A'CCGG_T Cuts at: 3073 3073 Size: 4685 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 219 1276 1300 1450 1656 219 Size: 1057 24 150 206 3248 Fragments arranged by size: 3248 1057 206 150 24 AlwNI CAG_nnn'CTG Cuts at: 951 1990 3557 951 Size: 1039 1567 2079 Fragments arranged by size: 2079 1567 1039 ApaI G_GGCC'C Cuts at: 811 1603 811 Size: 792 3893 ApaLI G'TGCA_C Cuts at: 3455 3455 Size: 4685 ApoI r'AATT_y Cuts at: 1063 1689 4038 4294 1063 Size: 626 2349 256 1454 Fragments arranged by size: 2349 1454 626 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4685 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4685 AvaI C'yCGr_G Cuts at: 1705 1705 Size: 4685 BamHI G'GATC_C Cuts at: 1518 1710 1518 Size: 192 4493 BanI G'GyrC_C Cuts at: 203 1613 1701 2115 2363 2398 203 Size: 1410 88 414 248 35 2490 Fragments arranged by size: 2490 1410 414 248 88 35 BanII G_rGCy'C Cuts at: 193 811 1010 1487 1603 1699 2729 193 Size: 618 199 477 116 96 1030 2149 Fragments arranged by size: 2149 1030 618 477 199 116 96 BbeI G_GCGC'C Cuts at: 2119 2367 2119 Size: 248 4437 BbsI GAAGACnn'nnnn_ Cuts at: 223 388 223 Size: 165 4520 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1632 1666 1632 Size: 34 4651 BciVI GTATCCnnnnn_n' Cuts at: 2583 3344 4685 2583 Size: 761 1341 2583 Fragments arranged by size: 2583 1341 761 BclI T'GATC_A Cuts at: 1461 2205 1461 Size: 744 3941 BglI GCCn_nnn'nGGC Cuts at: 140 967 140 Size: 827 3858 BglII A'GATC_T Cuts at: 896 2200 4237 896 Size: 1304 2037 1344 Fragments arranged by size: 2037 1344 1304 BlpI GC'TnA_GC Cuts at: 1797 1797 Size: 4685 Bme1580I G_kGCm'C Cuts at: 811 1603 2310 2403 3459 811 Size: 792 707 93 1056 2037 Fragments arranged by size: 2037 1056 792 707 93 BmrI ACTGGGnnnn_n' Cuts at: 1632 1897 1978 2313 4457 1632 Size: 265 81 335 2144 1860 Fragments arranged by size: 2144 1860 335 265 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 519 3052 519 Size: 2533 2152 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 514 589 1246 1396 514 Size: 75 657 150 3803 Fragments arranged by size: 3803 657 150 75 Bpu10I CC'TnA_GC Cuts at: 1241 1241 Size: 4685 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1435 1861 2740 3232 3530 1435 Size: 426 879 492 298 2590 Fragments arranged by size: 2590 879 492 426 298 BsaI GGTCTCn'nnnn_ Cuts at: 4389 4389 Size: 4685 BsaAI yAC'GTr Cuts at: 1196 1305 2038 1196 Size: 109 733 3843 Fragments arranged by size: 3843 733 109 BsaBI GATnn'nnATC Cuts at: 6 1498 2199 4236 6 Size: 1492 701 2037 455 Fragments arranged by size: 2037 1492 701 455 BsaHI Gr'CG_yC Cuts at: 223 2116 2364 3066 4562 223 Size: 1893 248 702 1496 346 Fragments arranged by size: 1893 1496 702 346 248 BsaWI w'CCGG_w Cuts at: 1874 2395 3073 3347 3494 3905 1874 Size: 521 678 274 147 411 2654 Fragments arranged by size: 2654 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1189 1219 1189 Size: 30 4655 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 370 373 628 631 805 1243 370 Size: 3 255 3 174 438 3812 Fragments arranged by size: 3812 438 255 174 3 3 BseYI C'CCAG_C Cuts at: 674 1029 2110 2868 3445 3941 674 Size: 355 1081 758 577 496 1418 Fragments arranged by size: 1418 1081 758 577 496 355 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1246 1272 1274 1246 Size: 26 2 4657 Fragments arranged by size: 4657 26 2 BsiEI CG_ry'CG Cuts at: 104 2227 2273 3481 104 Size: 2123 46 1208 1308 Fragments arranged by size: 2123 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 505 1147 1699 2477 2667 3459 505 Size: 642 552 778 190 792 1731 Fragments arranged by size: 1731 792 778 642 552 190 BsiWI C'GTAC_G Cuts at: 1196 1196 Size: 4685 BsmI GAATG_Cn' Cuts at: 184 710 184 Size: 526 4159 Bsp1286I G_dGCh'C Cuts at: 193 505 811 1010 1147 1487 1603 1699 2310 Size: 312 306 199 137 340 116 96 611 Cuts at: 2310 2403 2477 2667 2729 3459 193 Size: 93 74 190 62 730 1419 Fragments arranged by size: 1419 730 611 340 312 306 199 190 137 116 96 93 74 62 BspEI T'CCGG_A Cuts at: 3905 3905 Size: 4685 BspHI T'CATG_A Cuts at: 3861 3861 Size: 4685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1735 1949 2251 2632 1735 Size: 214 302 381 3788 Fragments arranged by size: 3788 381 302 214 BsrBI CCG'CTC Cuts at: 50 2977 3031 4515 4680 50 Size: 2927 54 1484 165 55 Fragments arranged by size: 2927 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1650 1656 2597 1650 Size: 6 941 3738 Fragments arranged by size: 3738 941 6 BsrFI r'CCGG_y Cuts at: 154 2683 2864 3073 154 Size: 2529 181 209 1766 Fragments arranged by size: 2529 1766 209 181 BssSI C'ACGA_G Cuts at: 1021 2956 3314 1021 Size: 1935 358 2392 Fragments arranged by size: 2392 1935 358 BstAPI GCAn_nnn'nTGC Cuts at: 252 2062 252 Size: 1810 2875 BstBI TT'CG_AA Cuts at: 3046 3046 Size: 4685 BstEII G'GTnAC_C Cuts at: 1080 1080 Size: 4685 BstXI CCAn_nnnn'nTGG Cuts at: 138 1224 1610 138 Size: 1086 386 3213 Fragments arranged by size: 3213 1086 386 BstYI r'GATC_y Cuts at: 1 362 581 896 1400 1499 1518 1710 2177 Size: 361 219 315 504 99 19 192 467 Cuts at: 2177 2200 2535 2781 3793 3879 3891 4237 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 1012 504 467 449 361 346 335 315 246 219 192 99 86 23 19 12 Bsu36I CC'TnA_GG Cuts at: 650 650 Size: 4685 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2635 2816 2979 2635 Size: 181 163 4341 Fragments arranged by size: 4341 181 163 BtsI GCAGTG_nn' Cuts at: 203 245 246 1462 1928 203 Size: 42 1 1216 466 2960 Fragments arranged by size: 2960 1216 466 42 1 DraI TTT'AAA Cuts at: 1684 1684 Size: 4685 DrdI GACnn_nn'nnGTC Cuts at: 2391 3249 2391 Size: 858 3827 EaeI y'GGCC_r Cuts at: 128 623 818 2270 2444 2835 2862 4207 128 Size: 495 195 1452 174 391 27 1345 606 Fragments arranged by size: 1452 1345 606 495 391 195 174 27 EagI C'GGCC_G Cuts at: 2270 2270 Size: 4685 EarI CTCTTCn'nnn_ Cuts at: 124 1166 2708 2918 124 Size: 1042 1542 210 1891 Fragments arranged by size: 1891 1542 1042 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2893 3203 3349 4495 2893 Size: 310 146 1146 3083 Fragments arranged by size: 3083 1146 310 146 EcoICRI GAG'CTC Cuts at: 1697 1697 Size: 4685 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 358 514 589 1246 1396 2510 2942 3689 3957 Size: 156 75 657 150 1114 432 747 268 Cuts at: 3957 358 Size: 1086 Fragments arranged by size: 1114 1086 747 657 432 268 156 150 75 EcoNI CCTnn'n_nnAGG Cuts at: 1072 1225 1507 1072 Size: 153 282 4250 Fragments arranged by size: 4250 282 153 EcoO109I rG'GnC_Cy Cuts at: 808 1026 1599 1824 808 Size: 218 573 225 3669 Fragments arranged by size: 3669 573 225 218 EcoRI G'AATT_C Cuts at: 1063 1689 1063 Size: 626 4059 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2704 2704 Size: 4685 FspI TGC'GCA Cuts at: 2466 2466 Size: 4685 HaeII r_GCGC'y Cuts at: 2119 2367 3389 4368 2119 Size: 248 1022 979 2436 Fragments arranged by size: 2436 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2527 2557 2559 2589 2527 Size: 30 2 30 4623 Fragments arranged by size: 4623 30 30 2 HincII GTy'rAC Cuts at: 1724 1724 Size: 4685 KasI G'GCGC_C Cuts at: 2115 2363 2115 Size: 248 4437 KpnI G_GTAC'C Cuts at: 1705 1705 Size: 4685 MluI A'CGCG_T Cuts at: 4118 4118 Size: 4685 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 251 1148 3356 3540 251 Size: 897 2208 184 1396 Fragments arranged by size: 2208 1396 897 184 MscI TGG'CCA Cuts at: 130 625 2446 130 Size: 495 1821 2369 Fragments arranged by size: 2369 1821 495 MslI CAynn'nnrTG Cuts at: 715 2674 2801 715 Size: 1959 127 2599 Fragments arranged by size: 2599 1959 127 MspA1I CmG'CkG Cuts at: 325 567 1796 2110 2470 3483 3728 325 Size: 242 1229 314 360 1013 245 1282 Fragments arranged by size: 1282 1229 1013 360 314 245 242 NaeI GCC'GGC Cuts at: 156 2866 156 Size: 2710 1975 NarI GG'CG_CC Cuts at: 2116 2364 2116 Size: 248 4437 NdeI CA'TA_TG Cuts at: 301 826 301 Size: 525 4160 NgoMIV G'CCGG_C Cuts at: 154 2864 154 Size: 2710 1975 NspI r_CATG'y Cuts at: 414 784 885 1260 1738 2238 3145 414 Size: 370 101 375 478 500 907 1954 Fragments arranged by size: 1954 907 500 478 375 370 101 PciI A'CATG_T Cuts at: 410 780 3141 410 Size: 370 2361 1954 Fragments arranged by size: 2361 1954 370 PflMI CCAn_nnn'nTGG Cuts at: 771 1185 771 Size: 414 4271 PfoI T'CCnGG_A Cuts at: 458 458 Size: 4685 PmeI GTTT'AAAC Cuts at: 1684 1684 Size: 4685 PmlI CAC'GTG Cuts at: 1305 1305 Size: 4685 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 3824 Size: 32 4653 PpuMI rG'GwC_Cy Cuts at: 1026 1026 Size: 4685 PshAI GACnn'nnGTC Cuts at: 952 952 Size: 4685 PspOMI G'GGCC_C Cuts at: 807 1599 807 Size: 792 3893 PstI C_TGCA'G Cuts at: 257 1429 1732 2417 257 Size: 1172 303 685 2525 Fragments arranged by size: 2525 1172 685 303 PvuI CG_AT'CG Cuts at: 104 104 Size: 4685 PvuII CAG'CTG Cuts at: 325 567 2110 2470 325 Size: 242 1543 360 2540 Fragments arranged by size: 2540 1543 360 242 RsrII CG'GwC_CG Cuts at: 2880 2880 Size: 4685 SacI G_AGCT'C Cuts at: 1699 1699 Size: 4685 SalI G'TCGA_C Cuts at: 1722 1722 Size: 4685 SapI GCTCTTCn'nnn_ Cuts at: 2708 2918 2708 Size: 210 4475 SbfI CC_TGCA'GG Cuts at: 1732 1732 Size: 4685 ScaI AGT'ACT Cuts at: 4202 4202 Size: 4685 SfcI C'TryA_G Cuts at: 33 253 1425 1728 2413 3406 3597 33 Size: 220 1172 303 685 993 191 1121 Fragments arranged by size: 1172 1121 993 685 303 220 191 SfoI GGC'GCC Cuts at: 2117 2365 2117 Size: 248 4437 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4685 SmaI CCC'GGG Cuts at: 1707 1707 Size: 4685 SmlI C'TyrA_G Cuts at: 1414 1840 2755 3247 3509 1414 Size: 426 915 492 262 2590 Fragments arranged by size: 2590 915 492 426 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4685 SphI G_CATG'C Cuts at: 1260 1738 2238 1260 Size: 478 500 3707 Fragments arranged by size: 3707 500 478 StuI AGG'CCT Cuts at: 769 769 Size: 4685 StyI C'CwwG_G Cuts at: 165 770 890 1819 2173 165 Size: 605 120 929 354 2677 Fragments arranged by size: 2677 929 605 354 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2268 2401 2860 3069 2268 Size: 133 459 209 3884 Fragments arranged by size: 3884 459 209 133 TatI w'GTAC_w Cuts at: 4200 4200 Size: 4685 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4202 4234 4202 Size: 32 4653 Tth111I GACn'n_nGTC Cuts at: 222 313 2482 222 Size: 91 2169 2425 Fragments arranged by size: 2425 2169 91 XbaI T'CTAG_A Cuts at: 1716 1716 Size: 4685 XcmI CCAnnnn_n'nnnnTGG Cuts at: 948 2180 948 Size: 1232 3453 XmaI C'CCGG_G Cuts at: 1705 1705 Size: 4685 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BsmBI BsrGI BssHII BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI NheI NotI NruI NsiI PacI PasI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI