(Linear) (Six-base) MAPSORT of: KIEE2764.seq Check: 9847 from: 1 to: 1572 KIEE2764.orf With 182 enzymes: SgfI * July 3, 2020 20:21 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 1572 Size: 250 1322 AflIII A'CryG_T Cuts at: 0 302 672 1085 1572 Size: 302 370 413 487 Fragments arranged by size: 487 413 370 302 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 111 1168 1192 1342 1548 1572 Size: 111 1057 24 150 206 24 Fragments arranged by size: 1057 206 150 111 24 24 AlwNI CAG_nnn'CTG Cuts at: 0 843 1572 Size: 843 729 ApaI G_GGCC'C Cuts at: 0 703 1495 1572 Size: 703 792 77 Fragments arranged by size: 792 703 77 ApoI r'AATT_y Cuts at: 0 955 1572 Size: 955 617 BamHI G'GATC_C Cuts at: 0 1410 1572 Size: 1410 162 BanI G'GyrC_C Cuts at: 0 95 1505 1572 Size: 95 1410 67 Fragments arranged by size: 1410 95 67 BanII G_rGCy'C Cuts at: 0 85 703 902 1379 1495 1572 Size: 85 618 199 477 116 77 Fragments arranged by size: 618 477 199 116 85 77 BbsI GAAGACnn'nnnn_ Cuts at: 0 115 280 1572 Size: 115 165 1292 Fragments arranged by size: 1292 165 115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1524 1558 1572 Size: 1524 34 14 Fragments arranged by size: 1524 34 14 BclI T'GATC_A Cuts at: 0 1353 1572 Size: 1353 219 BglI GCCn_nnn'nGGC Cuts at: 0 32 859 1572 Size: 32 827 713 Fragments arranged by size: 827 713 32 BglII A'GATC_T Cuts at: 0 788 1572 Size: 788 784 Bme1580I G_kGCm'C Cuts at: 0 703 1495 1572 Size: 703 792 77 Fragments arranged by size: 792 703 77 BmrI ACTGGGnnnn_n' Cuts at: 0 1524 1572 Size: 1524 48 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 411 1572 Size: 411 1161 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 406 481 1138 1288 1572 Size: 406 75 657 150 284 Fragments arranged by size: 657 406 284 150 75 Bpu10I CC'TnA_GC Cuts at: 0 1133 1572 Size: 1133 439 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1327 1572 Size: 1327 245 BsaAI yAC'GTr Cuts at: 0 1088 1197 1572 Size: 1088 109 375 Fragments arranged by size: 1088 375 109 BsaBI GATnn'nnATC Cuts at: 0 1390 1572 Size: 1390 182 BsaHI Gr'CG_yC Cuts at: 0 115 1572 Size: 115 1457 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1081 1111 1572 Size: 1081 30 461 Fragments arranged by size: 1081 461 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 262 265 520 523 697 1135 1572 Size: 262 3 255 3 174 438 437 Fragments arranged by size: 438 437 262 255 174 3 3 BseYI C'CCAG_C Cuts at: 0 566 921 1572 Size: 566 355 651 Fragments arranged by size: 651 566 355 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1138 1164 1166 1572 Size: 1138 26 2 406 Fragments arranged by size: 1138 406 26 2 BsiHKAI G_wGCw'C Cuts at: 0 397 1039 1572 Size: 397 642 533 Fragments arranged by size: 642 533 397 BsiWI C'GTAC_G Cuts at: 0 1088 1572 Size: 1088 484 BsmI GAATG_Cn' Cuts at: 0 76 602 1572 Size: 76 526 970 Fragments arranged by size: 970 526 76 Bsp1286I G_dGCh'C Cuts at: 0 85 397 703 902 1039 1379 1495 1572 Size: 85 312 306 199 137 340 116 77 Fragments arranged by size: 340 312 306 199 137 116 85 77 BsrDI GCAATG_nn' Cuts at: 0 1542 1548 1572 Size: 1542 6 24 Fragments arranged by size: 1542 24 6 BsrFI r'CCGG_y Cuts at: 0 46 1572 Size: 46 1526 BssSI C'ACGA_G Cuts at: 0 913 1572 Size: 913 659 BstAPI GCAn_nnn'nTGC Cuts at: 0 144 1572 Size: 144 1428 BstEII G'GTnAC_C Cuts at: 0 972 1572 Size: 972 600 BstXI CCAn_nnnn'nTGG Cuts at: 0 30 1116 1502 1572 Size: 30 1086 386 70 Fragments arranged by size: 1086 386 70 30 BstYI r'GATC_y Cuts at: 0 254 473 788 1292 1391 1410 1572 Size: 254 219 315 504 99 19 162 Fragments arranged by size: 504 315 254 219 162 99 19 Bsu36I CC'TnA_GG Cuts at: 0 542 1572 Size: 542 1030 BtsI GCAGTG_nn' Cuts at: 0 95 137 138 1354 1572 Size: 95 42 1 1216 218 Fragments arranged by size: 1216 218 95 42 1 EaeI y'GGCC_r Cuts at: 0 20 515 710 1572 Size: 20 495 195 862 Fragments arranged by size: 862 495 195 20 EarI CTCTTCn'nnn_ Cuts at: 0 16 1058 1572 Size: 16 1042 514 Fragments arranged by size: 1042 514 16 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 406 481 1138 1288 1572 Size: 250 156 75 657 150 284 Fragments arranged by size: 657 284 250 156 150 75 EcoNI CCTnn'n_nnAGG Cuts at: 0 964 1117 1399 1572 Size: 964 153 282 173 Fragments arranged by size: 964 282 173 153 EcoO109I rG'GnC_Cy Cuts at: 0 700 918 1491 1572 Size: 700 218 573 81 Fragments arranged by size: 700 573 218 81 EcoRI G'AATT_C Cuts at: 0 955 1572 Size: 955 617 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 143 1040 1572 Size: 143 897 532 Fragments arranged by size: 897 532 143 MscI TGG'CCA Cuts at: 0 22 517 1572 Size: 22 495 1055 Fragments arranged by size: 1055 495 22 MslI CAynn'nnrTG Cuts at: 0 607 1572 Size: 607 965 MspA1I CmG'CkG Cuts at: 0 217 459 1572 Size: 217 242 1113 Fragments arranged by size: 1113 242 217 NaeI GCC'GGC Cuts at: 0 48 1572 Size: 48 1524 NdeI CA'TA_TG Cuts at: 0 193 718 1572 Size: 193 525 854 Fragments arranged by size: 854 525 193 NgoMIV G'CCGG_C Cuts at: 0 46 1572 Size: 46 1526 NspI r_CATG'y Cuts at: 0 306 676 777 1152 1572 Size: 306 370 101 375 420 Fragments arranged by size: 420 375 370 306 101 PciI A'CATG_T Cuts at: 0 302 672 1572 Size: 302 370 900 Fragments arranged by size: 900 370 302 PflMI CCAn_nnn'nTGG Cuts at: 0 663 1077 1572 Size: 663 414 495 Fragments arranged by size: 663 495 414 PfoI T'CCnGG_A Cuts at: 0 350 1572 Size: 350 1222 PmlI CAC'GTG Cuts at: 0 1197 1572 Size: 1197 375 PpuMI rG'GwC_Cy Cuts at: 0 918 1572 Size: 918 654 PshAI GACnn'nnGTC Cuts at: 0 844 1572 Size: 844 728 PspOMI G'GGCC_C Cuts at: 0 699 1491 1572 Size: 699 792 81 Fragments arranged by size: 792 699 81 PstI C_TGCA'G Cuts at: 0 149 1321 1572 Size: 149 1172 251 Fragments arranged by size: 1172 251 149 PvuII CAG'CTG Cuts at: 0 217 459 1572 Size: 217 242 1113 Fragments arranged by size: 1113 242 217 SfcI C'TryA_G Cuts at: 0 145 1317 1572 Size: 145 1172 255 Fragments arranged by size: 1172 255 145 SmlI C'TyrA_G Cuts at: 0 1306 1572 Size: 1306 266 SphI G_CATG'C Cuts at: 0 1152 1572 Size: 1152 420 StuI AGG'CCT Cuts at: 0 661 1572 Size: 661 911 StyI C'CwwG_G Cuts at: 0 57 662 782 1572 Size: 57 605 120 790 Fragments arranged by size: 790 605 120 57 Tth111I GACn'n_nGTC Cuts at: 0 114 205 1572 Size: 114 91 1367 Fragments arranged by size: 1367 114 91 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 840 1572 Size: 840 732 Enzymes that do cut: AcuI AflIII AlfI AlwNI ApaI ApoI BamHI BanI BanII BbsI BcgI BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtsI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NspI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SfcI SmlI SphI StuI StyI Tth111I XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BciVI BfrBI BlpI BmgBI BmtI BsaI BsaWI BsiEI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BstBI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NheI NotI NruI NsiI PacI PasI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII TatI TstI XbaI XhoI XmaI XmnI ZraI