Restriction map for pF1KB6663
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspHI       AspI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BfaI        BfrI        
BglII       BlnI        BpuAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        EagI        EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRV       EcoT22I     
FseI        FspI        HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MamI        MluI        MroI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcsI          178  GAATTC               [GA]^AATT[CT]
AhdI          110  GACCAGTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         1075  GGATC                GGATC(4/5)
AlwNI         980  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          178  GAATTC               [GA]^AATT[CT]
AseI          439  ATTAAT               AT^TAAT
AsnI          439  ATTAAT               AT^TAAT
AspEI         110  GACCAGTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          150  TGGCCA               TGG^CCA
BanII         752  GAGCCC               G[GA]GC[CT]^C
BclI          227  TGATCA               T^GATCA
BglI          944  GCCCTCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          563  GCTCAGC              GC^T[ACGT]AGC
BmyI          752  GAGCCC               G[AGT]GC[ACT]^C
Bpu1102I      563  GCTCAGC              GC^T[ACGT]AGC
BsaAI          46  TACGTG               [CT]AC^GT[GA]
BsmAI         918  GTCTC                GTCTC(1/5)
BsmFI         401  GGGAC                GGGAC(10/14)
BsmI         1019  GAATGC               GAATGC(1/-1)
Bsp1286I      752  GAGCCC               G[AGT]GC[ACT]^C
BspHI         218  TCATGA               T^CATGA
BstAPI       1110  GCATGGTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1087  GGTCACC              G^GT[ACGT]ACC
BstPI        1087  GGTCACC              G^GT[ACGT]ACC
BstYI        1075  GGATCT               [GA]^GATC[CT]
CelII         563  GCTCAGC              GC^T[ACGT]AGC
DraIII        890  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          851  GACATCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      110  GACCAGTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1087  GGTCACC              G^GT[ACGT]ACC
EcoRI         178  GAATTC               G^AATTC
FbaI          227  TGATCA               T^GATCA
HincII        118  GTCAAC               GT[CT]^[GA]AC
HindII        118  GTCAAC               GT[CT]^[GA]AC
MaeII          47  ACGT                 A^CGT
MboII         193  GAAGA                GAAGA(8/7)
MfeI          473  CAATTG               C^AATTG
MflI         1075  GGATCT               [GA]^GATC[CT]
MluNI         150  TGGCCA               TGG^CCA
MscI          150  TGGCCA               TGG^CCA
MunI          473  CAATTG               C^AATTG
PaeR7I       1241  CTCGAG               C^TCGAG
PshBI         439  ATTAAT               AT^TAAT
PstI          809  CTGCAG               CTGCA^G
PvuII         625  CAGCTG               CAG^CTG
RcaI          218  TCATGA               T^CATGA
SfcI          809  CTGCAG               C^T[GA][CT]AG
TfiI           27  GAATC                G^A[AT]TC
XhoI         1241  CTCGAG               C^TCGAG
XhoII        1075  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI           45  GTAC                 GT^AC
              495  GTAC                
Asp700I        91  GAACTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              574  GAACGACTTC          
AvaI         1058  CCCGAG               C^[CT]CG[GA]G
             1241  CTCGAG              
BciVI         767  GGATAC               GTATCC(6/5)
             1150  GGATAC              
BpmI          100  CTCCAG               CTGGAG(16/14)
              749  CTGGAG              
BsoBI        1058  CCCGAG               C^[CT]CG[GA]G
             1241  CTCGAG              
BsrBI         598  CCGCTC               CCGCTC(-3/-3)
               22  GAGCGG              
BstXI         159  CCAAGGCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              662  CCAATCCTGTGG        
CfoI          362  GCGC                 GCG^C
              555  GCGC                
EaeI          150  TGGCCA               [CT]^GGCC[GA]
             1225  CGGCCA              
EcoNI         406  CCTGACACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              697  CCTCCACCAGG         
HaeII         361  AGCGCC               [GA]GCGC^[CT]
              554  AGCGCC              
HhaI          362  GCGC                 GCG^C
              555  GCGC                
HinP1I        362  GCGC                 G^CGC
              555  GCGC                
MseI          212  TTAA                 T^TAA
              440  TTAA                
MslI          123  CACCCACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              219  CATGAACATG          
PleI          541  GACTC                GAGTC(4/5)
              143  GAGTC               
PpuMI         401  GGGACCC              [GA]G^G[AT]CC[CT]
             1095  AGGTCCT             
RsaI           45  GTAC                 GT^AC
              495  GTAC                
SmlI          183  CTTGAG               C^T[CT][GA]AG
             1241  CTCGAG              
SphI          707  GCATGC               GCATG^C
             1049  GCATGC              
TaqI         1242  TCGA                 T^CGA
             1254  TCGA                
Tru9I         212  TTAA                 T^TAA
              440  TTAA                
Tsp45I        145  GTCAC                ^GT[GC]AC
             1088  GTCAC               
TthHB8I      1242  TCGA                 T^CGA
             1254  TCGA                
XmnI           91  GAACTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              574  GAACGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BcnI        
BsaJI       BseRI       BsiYI       BslI        BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TseI        Tsp509I     TspRI       Van91I      XcmI        
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