Restriction map for pF1KE2649
 (Circular) (Six-base) MAPSORT of: pF1KE2649.seq  Check: 2105  from: 1  to: 3686



pF1KE2649  3686 bp

 With 182 enzymes: SgfI * 

                        November 11, 2016 16:52  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    656     656 
   Size:       3686
 
AccI GT'mk_AC

Cuts at:    724     724 
   Size:       3686
 
Acc65I G'GTAC_C

Cuts at:    702     702 
   Size:       3686
 
AclI AA'CG_TT

Cuts at:   3526    3526 
   Size:       3686
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    574    1511    1943    2690    2958     574 
   Size:        937     432     747     268    1302

  Fragments arranged by size: 

                1302     937     747     432     268
 
AflIII A'CryG_T

Cuts at:    157    1038    2142    3119     157 
   Size:        881    1104     977     724

  Fragments arranged by size: 

                1104     977     881     724
 
AgeI A'CCGG_T

Cuts at:   2074    2074 
   Size:       3686
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    688     688 
   Size:       3686
 
AlwNI CAG_nnn'CTG

Cuts at:    529     991    2558     529 
   Size:        462    1567    1657

  Fragments arranged by size: 

                1657    1567     462
 
ApaLI G'TGCA_C

Cuts at:   2456    2456 
   Size:       3686
 
ApoI r'AATT_y

Cuts at:    187     690    3039    3295     187 
   Size:        503    2349     256     578

  Fragments arranged by size: 

                2349     578     503     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3686
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3686
 
AvaI C'yCGr_G

Cuts at:    706     706 
   Size:       3686
 
BamHI G'GATC_C

Cuts at:    711     711 
   Size:       3686
 
BanI G'GyrC_C

Cuts at:    399     702    1116    1364    1399     399 
   Size:        303     414     248      35    2686

  Fragments arranged by size: 

                2686     414     303     248      35
 
BanII G_rGCy'C

Cuts at:    700    1730     700 
   Size:       1030    2656
 
BbeI G_GCGC'C

Cuts at:   1120    1368    1120 
   Size:        248    3438
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3652
 
BciVI GTATCCnnnnn_n'

Cuts at:   1584    2345    3686    1584 
   Size:        761    1341    1584

  Fragments arranged by size: 

                1584    1341     761
 
BclI T'GATC_A

Cuts at:   1206    1206 
   Size:       3686
 
BglII A'GATC_T

Cuts at:   1201    3238    1201 
   Size:       2037    1649
 
BlpI GC'TnA_GC

Cuts at:    798     798 
   Size:       3686
 
Bme1580I G_kGCm'C

Cuts at:   1311    1404    2460    1311 
   Size:         93    1056    2537

  Fragments arranged by size: 

                2537    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    898     979    1314    3458     898 
   Size:         81     335    2144    1126

  Fragments arranged by size: 

                2144    1126     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2053    2053 
   Size:       3686
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    506     629     658     506 
   Size:        123      29    3534

  Fragments arranged by size: 

                3534     123      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    862    1741    2233    2531     862 
   Size:        879     492     298    2017

  Fragments arranged by size: 

                2017     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    366    3390     366 
   Size:       3024     662
 
BsaAI yAC'GTr

Cuts at:   1039    1039 
   Size:       3686
 
BsaBI GATnn'nnATC

Cuts at:      6    1200    3237       6 
   Size:       1194    2037     455

  Fragments arranged by size: 

                2037    1194     455
 
BsaHI Gr'CG_yC

Cuts at:   1117    1365    2067    3563    1117 
   Size:        248     702    1496    1240

  Fragments arranged by size: 

                1496    1240     702     248
 
BsaWI w'CCGG_w

Cuts at:    875    1396    2074    2348    2495    2906     875 
   Size:        521     678     274     147     411    1655

  Fragments arranged by size: 

                1655     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1111    1869    2446    2942    1111 
   Size:        758     577     496    1855

  Fragments arranged by size: 

                1855     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    566     566 
   Size:       3686
 
BsiEI CG_ry'CG

Cuts at:    104     313    1228    1274    2482     104 
   Size:        209     915      46    1208    1308

  Fragments arranged by size: 

                1308    1208     915     209      46
 
BsiHKAI G_wGCw'C

Cuts at:    700    1478    1668    2460     700 
   Size:        778     190     792    1926

  Fragments arranged by size: 

                1926     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    700    1311    1404    1478    1668    1730    2460     700 
   Size:        611      93      74     190      62     730    1926

  Fragments arranged by size: 

                1926     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2906    2906 
   Size:       3686
 
BspHI T'CATG_A

Cuts at:   2862    2862 
   Size:       3686
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    656     736     950    1252    1633     656 
   Size:         80     214     302     381    2709

  Fragments arranged by size: 

                2709     381     302     214      80
 
BsrBI CCG'CTC

Cuts at:     50    1978    2032    3516    3681      50 
   Size:       1928      54    1484     165      55

  Fragments arranged by size: 

                1928    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1598    1598 
   Size:       3686
 
BsrFI r'CCGG_y

Cuts at:   1684    1865    2074    1684 
   Size:        181     209    3296

  Fragments arranged by size: 

                3296     209     181
 
BssSI C'ACGA_G

Cuts at:   1957    2315    1957 
   Size:        358    3328
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1063    1063 
   Size:       3686
 
BstBI TT'CG_AA

Cuts at:   2047    2047 
   Size:       3686
 
BstYI r'GATC_y

Cuts at:      1     711    1178    1201    1536    1782    2794    2880    2892 
   Size:        710     467      23     335     246    1012      86      12

Cuts at:   2892    3238       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     710     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    152    1636    1817    1980     152 
   Size:       1484     181     163    1858

  Fragments arranged by size: 

                1858    1484     181     163
 
BtsI GCAGTG_nn'

Cuts at:    929     929 
   Size:       3686
 
DraI TTT'AAA

Cuts at:    685     685 
   Size:       3686
 
DrdI GACnn_nn'nnGTC

Cuts at:   1392    2250    1392 
   Size:        858    2828
 
EaeI y'GGCC_r

Cuts at:   1271    1445    1836    1863    3208    1271 
   Size:        174     391      27    1345    1749

  Fragments arranged by size: 

                1749    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1271    1271 
   Size:       3686
 
EarI CTCTTCn'nnn_

Cuts at:    652    1709    1919     652 
   Size:       1057     210    2419

  Fragments arranged by size: 

                2419    1057     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1894    2204    2350    3496    1894 
   Size:        310     146    1146    2084

  Fragments arranged by size: 

                2084    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    698     698 
   Size:       3686
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    506     574     629     658    1511    1943    2690    2958     506 
   Size:         68      55      29     853     432     747     268    1234

  Fragments arranged by size: 

                1234     853     747     432     268      68      55      29
            
 
EcoO109I rG'GnC_Cy

Cuts at:    825     825 
   Size:       3686
 
EcoRI G'AATT_C

Cuts at:    690     690 
   Size:       3686
 
EcoRV GAT'ATC

Cuts at:    170     170 
   Size:       3686
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1705    1705 
   Size:       3686
 
FspI TGC'GCA

Cuts at:   1467    1467 
   Size:       3686
 
HaeII r_GCGC'y

Cuts at:   1120    1368    2390    3369    1120 
   Size:        248    1022     979    1437

  Fragments arranged by size: 

                1437    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    460     492    1528    1558    1560    1590     460 
   Size:         32    1036      30       2      30    2556

  Fragments arranged by size: 

                2556    1036      32      30      30       2
 
HincII GTy'rAC

Cuts at:    725     725 
   Size:       3686
 
KasI G'GCGC_C

Cuts at:   1116    1364    1116 
   Size:        248    3438
 
KpnI G_GTAC'C

Cuts at:    706     706 
   Size:       3686
 
MluI A'CGCG_T

Cuts at:   3119    3119 
   Size:       3686
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2357    2541    2357 
   Size:        184    3502
 
MscI TGG'CCA

Cuts at:   1447    1447 
   Size:       3686
 
MslI CAynn'nnrTG

Cuts at:    238    1675    1802     238 
   Size:       1437     127    2122

  Fragments arranged by size: 

                2122    1437     127
 
MspA1I CmG'CkG

Cuts at:    315     797    1111    1471    2484    2729     315 
   Size:        482     314     360    1013     245    1272

  Fragments arranged by size: 

                1272    1013     482     360     314     245
 
NaeI GCC'GGC

Cuts at:   1867    1867 
   Size:       3686
 
NarI GG'CG_CC

Cuts at:   1117    1365    1117 
   Size:        248    3438
 
NgoMIV G'CCGG_C

Cuts at:   1865    1865 
   Size:       3686
 
NspI r_CATG'y

Cuts at:    161     739    1239    2146     161 
   Size:        578     500     907    1701

  Fragments arranged by size: 

                1701     907     578     500
 
PciI A'CATG_T

Cuts at:    157    2142     157 
   Size:       1985    1701
 
PmeI GTTT'AAAC

Cuts at:    685     685 
   Size:       3686
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2825    2857    2825 
   Size:         32    3654
 
PstI C_TGCA'G

Cuts at:    285     733    1418     285 
   Size:        448     685    2553

  Fragments arranged by size: 

                2553     685     448
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3686
 
PvuII CAG'CTG

Cuts at:   1111    1471    1111 
   Size:        360    3326
 
RsrII CG'GwC_CG

Cuts at:   1881    1881 
   Size:       3686
 
SacI G_AGCT'C

Cuts at:    700     700 
   Size:       3686
 
SalI G'TCGA_C

Cuts at:    723     723 
   Size:       3686
 
SapI GCTCTTCn'nnn_

Cuts at:    652    1709    1919     652 
   Size:       1057     210    2419

  Fragments arranged by size: 

                2419    1057     210
 
SbfI CC_TGCA'GG

Cuts at:    733     733 
   Size:       3686
 
ScaI AGT'ACT

Cuts at:   3203    3203 
   Size:       3686
 
SfcI C'TryA_G

Cuts at:     33     281     729    1414    2407    2598      33 
   Size:        248     448     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     448     248     191
 
SfoI GGC'GCC

Cuts at:   1118    1366    1118 
   Size:        248    3438
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3686
 
SmaI CCC'GGG

Cuts at:    708     708 
   Size:       3686
 
SmlI C'TyrA_G

Cuts at:    841    1756    2248    2510     841 
   Size:        915     492     262    2017

  Fragments arranged by size: 

                2017     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3686
 
SphI G_CATG'C

Cuts at:    739    1239     739 
   Size:        500    3186
 
SspI AAT'ATT

Cuts at:    626     626 
   Size:       3686
 
StuI AGG'CCT

Cuts at:    599     599 
   Size:       3686
 
StyI C'CwwG_G

Cuts at:    820    1174     820 
   Size:        354    3332
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    387    1269    1402    1861    2070     387 
   Size:        882     133     459     209    2003

  Fragments arranged by size: 

                2003     882     459     209     133
 
TatI w'GTAC_w

Cuts at:   3201    3201 
   Size:       3686
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3203    3235    3203 
   Size:         32    3654
 
Tth111I GACn'n_nGTC

Cuts at:   1483    1483 
   Size:       3686
 
XbaI T'CTAG_A

Cuts at:    717     717 
   Size:       3686
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1181    1181 
   Size:       3686
 
XmaI C'CCGG_G

Cuts at:    706     706 
   Size:       3686
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I    BsaXI    BseRI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII
    EcoNI     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SwaI     XhoI
     XmnI     ZraI
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