Restriction map for pF1KB7613
 (Linear) (Six-base) MAPSORT of: KIBB7613.seq  Check: 5125  from: 1  to: 909



KIBB7613.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AatII G_ACGT'C

Cuts at:      0     230     909 
   Size:        230     679
 
AccI GT'mk_AC

Cuts at:      0     287     909 
   Size:        287     622
 
Acc65I G'GTAC_C

Cuts at:      0     536     780     909 
   Size:        536     244     129

  Fragments arranged by size: 

                 536     244     129
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     674     909 
   Size:        442     232     235

  Fragments arranged by size: 

                 442     235     232
 
AflIII A'CryG_T

Cuts at:      0     587     909 
   Size:        587     322
 
AlwNI CAG_nnn'CTG

Cuts at:      0      28     813     909 
   Size:         28     785      96

  Fragments arranged by size: 

                 785      96      28
 
ApaI G_GGCC'C

Cuts at:      0     761     909 
   Size:        761     148
 
AvaI C'yCGr_G

Cuts at:      0     716     909 
   Size:        716     193
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     520     553     909 
   Size:        520      33     356

  Fragments arranged by size: 

                 520     356      33
 
BanI G'GyrC_C

Cuts at:      0      43     318     536     780     909 
   Size:         43     275     218     244     129

  Fragments arranged by size: 

                 275     244     218     129      43
 
BanII G_rGCy'C

Cuts at:      0     546     761     867     909 
   Size:        546     215     106      42

  Fragments arranged by size: 

                 546     215     106      42
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     735     909 
   Size:        735     174
 
BglII A'GATC_T

Cuts at:      0     417     909 
   Size:        417     492
 
Bme1580I G_kGCm'C

Cuts at:      0     761     909 
   Size:        761     148
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     760     909 
   Size:        760     149
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     683     909 
   Size:        208     475     226

  Fragments arranged by size: 

                 475     226     208
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     909 
   Size:         85     824
 
BsaHI Gr'CG_yC

Cuts at:      0     227     909 
   Size:        227     682
 
BsaWI w'CCGG_w

Cuts at:      0     903     909 
   Size:        903       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     140     143     152     808     909 
   Size:        140       3       9     656     101

  Fragments arranged by size: 

                 656     140     101       9       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     790     909 
   Size:        790     119
 
BsiHKAI G_wGCw'C

Cuts at:      0     546     807     867     909 
   Size:        546     261      60      42

  Fragments arranged by size: 

                 546     261      60      42
 
Bsp1286I G_dGCh'C

Cuts at:      0     546     761     807     867     909 
   Size:        546     215      46      60      42

  Fragments arranged by size: 

                 546     215      60      46      42
 
BspEI T'CCGG_A

Cuts at:      0     903     909 
   Size:        903       6
 
BsrGI T'GTAC_A

Cuts at:      0      59     909 
   Size:         59     850
 
BstEII G'GTnAC_C

Cuts at:      0     264     909 
   Size:        264     645
 
BstYI r'GATC_y

Cuts at:      0     354     417     909 
   Size:        354      63     492

  Fragments arranged by size: 

                 492     354      63
 
Bsu36I CC'TnA_GG

Cuts at:      0     298     909 
   Size:        298     611
 
BtgI C'CryG_G

Cuts at:      0     518     735     909 
   Size:        518     217     174

  Fragments arranged by size: 

                 518     217     174
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     314     349     563     598     909 
   Size:        314      35     214      35     311

  Fragments arranged by size: 

                 314     311     214      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0      93     909 
   Size:         93     816
 
EaeI y'GGCC_r

Cuts at:      0      84     482     909 
   Size:         84     398     427

  Fragments arranged by size: 

                 427     398      84
 
EarI CTCTTCn'nnn_

Cuts at:      0     643     909 
   Size:        643     266
 
EcoICRI GAG'CTC

Cuts at:      0     544     865     909 
   Size:        544     321      44

  Fragments arranged by size: 

                 544     321      44
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     442     674     683     909 
   Size:        208     234     232       9     226

  Fragments arranged by size: 

                 234     232     226     208       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     294     757     758     909 
   Size:        294     463       1     151

  Fragments arranged by size: 

                 463     294     151       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     654     909 
   Size:        654     255
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     339     371     609     641     909 
   Size:        339      32     238      32     268

  Fragments arranged by size: 

                 339     268     238      32      32
 
KpnI G_GTAC'C

Cuts at:      0     540     784     909 
   Size:        540     244     125

  Fragments arranged by size: 

                 540     244     125
 
MfeI C'AATT_G

Cuts at:      0     399     909 
   Size:        399     510
 
MscI TGG'CCA

Cuts at:      0      86     484     909 
   Size:         86     398     425

  Fragments arranged by size: 

                 425     398      86
 
MslI CAynn'nnrTG

Cuts at:      0     206     439     890     909 
   Size:        206     233     451      19

  Fragments arranged by size: 

                 451     233     206      19
 
MspA1I CmG'CkG

Cuts at:      0     810     909 
   Size:        810      99
 
NdeI CA'TA_TG

Cuts at:      0     570     909 
   Size:        570     339
 
NspI r_CATG'y

Cuts at:      0     286     591     909 
   Size:        286     305     318

  Fragments arranged by size: 

                 318     305     286
 
PciI A'CATG_T

Cuts at:      0     587     909 
   Size:        587     322
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      93     909 
   Size:         93     816
 
PpuMI rG'GwC_Cy

Cuts at:      0     294     909 
   Size:        294     615
 
PspOMI G'GGCC_C

Cuts at:      0     757     909 
   Size:        757     152
 
PstI C_TGCA'G

Cuts at:      0     563     872     909 
   Size:        563     309      37

  Fragments arranged by size: 

                 563     309      37
 
PvuII CAG'CTG

Cuts at:      0     810     909 
   Size:        810      99
 
SacI G_AGCT'C

Cuts at:      0     546     867     909 
   Size:        546     321      42

  Fragments arranged by size: 

                 546     321      42
 
SfcI C'TryA_G

Cuts at:      0     492     559     868     909 
   Size:        492      67     309      41

  Fragments arranged by size: 

                 492     309      67      41
 
SmlI C'TyrA_G

Cuts at:      0     100     716     909 
   Size:        100     616     193

  Fragments arranged by size: 

                 616     193     100
 
StuI AGG'CCT

Cuts at:      0     143     380     909 
   Size:        143     237     529

  Fragments arranged by size: 

                 529     237     143
 
TatI w'GTAC_w

Cuts at:      0      59     909 
   Size:         59     850
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     707     909 
   Size:        707     202
 
XhoI C'TCGA_G

Cuts at:      0     716     909 
   Size:        716     193
 
XmnI GAAnn'nnTTC

Cuts at:      0     554     909 
   Size:        554     355
 
ZraI GAC'GTC

Cuts at:      0     228     909 
   Size:        228     681
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI   AflIII    AlwNI     ApaI     AvaI
     BaeI     BanI    BanII    BciVI    BglII Bme1580I     BmrI     BpmI
    BpuEI    BsaHI    BsaWI    BseRI     BsgI  BsiHKAI Bsp1286I    BspEI
    BsrGI   BstEII    BstYI   Bsu36I     BtgI    CspCI   DraIII     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I     KpnI     MfeI
     MscI     MslI   MspA1I     NdeI     NspI     PciI    PflMI    PpuMI
   PspOMI     PstI    PvuII     SacI     SfcI     SmlI     StuI     TatI
     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII    BamHI
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaXI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
    BtgZI     BtsI     ClaI     DraI     DrdI     EagI     EciI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MluI     MmeI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XmaI
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