Restriction map for pF1KE4538
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       AccII       AclI        AcyI        AflII       
AgeI        AhdI        Alw44I      AocI        ApaI        
ApaLI       AscI        AspEI       AspI        AvaI        
AvrII       BbeI        BbrPI       BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaHI       BsaI        BsiEI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      CpoI        DraI        DraII       
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       FauI        FbaI        FseI        
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KspI        MfeI        MluI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I       1676  GGTACC               G^GTACC
AccI         1528  GTCTAC               GT^[AC][GT]AC
AccIII        812  TCCGGA               T^CCGGA
AseI         1714  ATTAAT               AT^TAAT
AsnI         1714  ATTAAT               AT^TAAT
Asp718I      1676  GGTACC               G^GTACC
AviII         308  TGCGCA               TGC^GCA
BalI          779  TGGCCA               TGG^CCA
BamHI        1752  GGATCC               G^GATCC
BanI         1676  GGTACC               G^G[CT][GA]CC
BbsI          607  GAAGAC               GAAGAC(2/6)
BbvCI         967  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1568  GCAAGCCAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         1444  CCGGG                CC^[GC]GG
BpuAI         607  GAAGAC               GAAGAC(2/6)
BsaAI        1454  CACGTA               [CT]AC^GT[GA]
BsaBI        1120  GATCCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         812  TCCGGA               [AT]^CCGG[AT]
BseAI         812  TCCGGA               T^CCGGA
BsgI          237  GTGCAG               GTGCAG(16/14)
BsmI         1502  GCATTC               GAATGC(1/-1)
BspEI         812  TCCGGA               T^CCGGA
DraIII        231  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I       806  CCAAGG               C^C[AT][AT]GG
EcoT22I      1312  ATGCAT               ATGCA^T
FspI          308  TGCGCA               TGC^GCA
KpnI         1676  GGTACC               GGTAC^C
MaeII        1455  ACGT                 A^CGT
MamI         1120  GATCCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         779  TGGCCA               TGG^CCA
MroI          812  TCCGGA               T^CCGGA
MscI          779  TGGCCA               TGG^CCA
NciI         1444  CCGGG                CC^[GC]GG
NsiI         1312  ATGCAT               ATGCA^T
PshBI        1714  ATTAAT               AT^TAAT
PstI           43  CTGCAG               CTGCA^G
PvuII         670  CAGCTG               CAG^CTG
SapI          451  GAAGAGC              GCTCTTC(1/4)
ScaI          908  AGTACT               AGT^ACT
SfcI           43  CTGCAG               C^T[GA][CT]AG
SspI          640  AATATT               AAT^ATT
StyI          806  CCAAGG               C^C[AT][AT]GG
Tsp45I        478  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcsI          398  AAATTC               [GA]^AATT[CT]
             1279  AAATTC              
AflIII        249  ACATGT               A^C[GA][CT]GT
              474  ACATGT              
AlwNI         355  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1574  CAGTCGCTG           
Aor51HI       819  AGCGCT               AGC^GCT
             1396  AGCGCT              
ApoI          398  AAATTC               [GA]^AATT[CT]
             1279  AAATTC              
Asp700I       459  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1109  GAAAAGCTTC          
AspHI         733  GAGCTC               G[AT]GC[AT]^C
             1648  GAGCTC              
BanII         733  GAGCTC               G[GA]GC[CT]^C
             1648  GAGCTC              
BsiHKAI       733  GAGCTC               G[AT]GC[AT]^C
             1648  GAGCTC              
BspDI        1127  ATCGAT               AT^CGAT
             1316  ATCGAT              
BspLU11I      249  ACATGT               A^CATGT
              474  ACATGT              
BstF5I       1662  CATCC                GGATG(2/0)
             1851  CATCC               
BstYI        1119  AGATCC               [GA]^GATC[CT]
             1752  GGATCC              
ClaI         1127  ATCGAT               AT^CGAT
             1316  ATCGAT              
EaeI          779  TGGCCA               [CT]^GGCC[GA]
             1490  CGGCCA              
Eco47III      819  AGCGCT               AGC^GCT
             1396  AGCGCT              
FokI         1662  CATCC                GGATG(9/13)
             1851  CATCC               
HaeII         819  AGCGCT               [GA]GCGC^[CT]
             1396  AGCGCT              
HapII         813  CCGG                 C^CGG
             1444  CCGG                
HpaII         813  CCGG                 C^CGG
             1444  CCGG                
HphI         1266  GGTGA                GGTGA(8/7)
               60  TCACC               
MflI         1119  AGATCC               [GA]^GATC[CT]
             1752  GGATCC              
MseI         1021  TTAA                 T^TAA
             1715  TTAA                
MslI          312  CACCCAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              783  CACCATTATG          
MspA1I        103  CAGCGG               C[AC]G^C[GT]G
              670  CAGCTG              
MspI          813  CCGG                 C^CGG
             1444  CCGG                
NspI          249  ACATGT               [GA]CATG^[CT]
              474  ACATGT              
PleI          191  GACTC                GAGTC(4/5)
               28  GAGTC               
SacI          733  GAGCTC               GAGCT^C
             1648  GAGCTC              
TfiI           90  GATTC                G^A[AT]TC
             1832  GAATC               
Tru9I        1021  TTAA                 T^TAA
             1715  TTAA                
XhoII        1119  AGATCC               [GA]^GATC[CT]
             1752  GGATCC              
XmnI          459  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1109  GAAAAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbvI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EarI        
EcoRII      Fnu4HI      HaeIII      HhaI        HinP1I      
HindIII     HinfI       ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TseI        Tsp509I     TspRI       TthHB8I     
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