Restriction map for pF1KE4538
 (Linear) (Six-base) MAPSORT of: KIEE4538.seq  Check: 878  from: 1  to: 1860



KIEE4538.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

AccI GT'mk_AC

Cuts at:      0    1529    1860 
   Size:       1529     331
 
Acc65I G'GTAC_C

Cuts at:      0    1676    1860 
   Size:       1676     184
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     202     382     779    1100    1375    1692    1771 
   Size:        188      14     180     397     321     275     317      79

Cuts at:   1771    1860 
   Size:         89

  Fragments arranged by size: 

                 397     321     317     275     188     180      89      79
                  14
 
AfeI AGC'GCT

Cuts at:      0     821    1398    1860 
   Size:        821     577     462

  Fragments arranged by size: 

                 821     577     462
 
AflIII A'CryG_T

Cuts at:      0     249     474    1860 
   Size:        249     225    1386

  Fragments arranged by size: 

                1386     249     225
 
AleI CACnn'nnGTG

Cuts at:      0     316    1860 
   Size:        316    1544
 
AlwNI CAG_nnn'CTG

Cuts at:      0     360    1579    1860 
   Size:        360    1219     281

  Fragments arranged by size: 

                1219     360     281
 
ApoI r'AATT_y

Cuts at:      0     398    1279    1860 
   Size:        398     881     581

  Fragments arranged by size: 

                 881     581     398
 
AseI AT'TA_AT

Cuts at:      0    1715    1860 
   Size:       1715     145
 
BamHI G'GATC_C

Cuts at:      0    1752    1860 
   Size:       1752     108
 
BanI G'GyrC_C

Cuts at:      0    1676    1860 
   Size:       1676     184
 
BanII G_rGCy'C

Cuts at:      0     737    1652    1860 
   Size:        737     915     208

  Fragments arranged by size: 

                 915     737     208
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     614    1860 
   Size:        614    1246
 
BbvCI CC'TCA_GC

Cuts at:      0     968    1860 
   Size:        968     892
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1557    1591    1860 
   Size:       1557      34     269

  Fragments arranged by size: 

                1557     269      34
 
BfrBI ATG'CAT

Cuts at:      0    1314    1860 
   Size:       1314     546
 
Bme1580I G_kGCm'C

Cuts at:      0     443    1860 
   Size:        443    1417
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     921    1860 
   Size:        921     939
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1198    1860 
   Size:       1198     662
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     976    1104    1168    1666    1681    1860 
   Size:        976     128      64     498      15     179

  Fragments arranged by size: 

                 976     498     179     128      64      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     968    1860 
   Size:        968     892
 
BsaAI yAC'GTr

Cuts at:      0    1456    1860 
   Size:       1456     404
 
BsaBI GATnn'nnATC

Cuts at:      0    1124    1860 
   Size:       1124     736
 
BsaWI w'CCGG_w

Cuts at:      0     812    1860 
   Size:        812    1048
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1511    1541    1860 
   Size:       1511      30     319

  Fragments arranged by size: 

                1511     319      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      18      40     529    1157    1391    1860 
   Size:         18      22     489     628     234     469

  Fragments arranged by size: 

                 628     489     469     234      22      18
 
BseYI C'CCAG_C

Cuts at:      0     442    1860 
   Size:        442    1418
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258    1860 
   Size:        258    1602
 
BsiHKAI G_wGCw'C

Cuts at:      0     737    1652    1860 
   Size:        737     915     208

  Fragments arranged by size: 

                 915     737     208
 
BsmI GAATG_Cn'

Cuts at:      0    1502    1860 
   Size:       1502     358
 
Bsp1286I G_dGCh'C

Cuts at:      0     443     737    1652    1860 
   Size:        443     294     915     208

  Fragments arranged by size: 

                 915     443     294     208
 
BspEI T'CCGG_A

Cuts at:      0     812    1860 
   Size:        812    1048
 
BstYI r'GATC_y

Cuts at:      0    1119    1752    1860 
   Size:       1119     633     108

  Fragments arranged by size: 

                1119     633     108
 
BtsI GCAGTG_nn'

Cuts at:      0     707    1860 
   Size:        707    1153
 
ClaI AT'CG_AT

Cuts at:      0    1128    1317    1860 
   Size:       1128     189     543

  Fragments arranged by size: 

                1128     543     189
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     875     910    1860 
   Size:        875      35     950

  Fragments arranged by size: 

                 950     875      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     236    1860 
   Size:        236    1624
 
EaeI y'GGCC_r

Cuts at:      0     779    1490    1860 
   Size:        779     711     370

  Fragments arranged by size: 

                 779     711     370
 
EarI CTCTTCn'nnn_

Cuts at:      0     164     446    1205    1860 
   Size:        164     282     759     655

  Fragments arranged by size: 

                 759     655     282     164
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      37    1860 
   Size:         37    1823
 
EcoICRI GAG'CTC

Cuts at:      0     735    1650    1860 
   Size:        735     915     210

  Fragments arranged by size: 

                 915     735     210
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     202     382     779     976    1100    1104    1168 
   Size:        188      14     180     397     197     124       4      64

Cuts at:   1168    1375    1666    1681    1692    1771    1860 
   Size:        207     291      15      11      79      89

  Fragments arranged by size: 

                 397     291     207     197     188     180     124      89
                  79      64      15      14      11       4
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1068    1860 
   Size:       1068     792
 
FspI TGC'GCA

Cuts at:      0     310    1860 
   Size:        310    1550
 
HaeII r_GCGC'y

Cuts at:      0     823    1400    1860 
   Size:        823     577     460

  Fragments arranged by size: 

                 823     577     460
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     418     450    1511    1543    1860 
   Size:        418      32    1061      32     317

  Fragments arranged by size: 

                1061     418     317      32      32
 
HindIII A'AGCT_T

Cuts at:      0     582     947    1112    1860 
   Size:        582     365     165     748

  Fragments arranged by size: 

                 748     582     365     165
 
KpnI G_GTAC'C

Cuts at:      0    1680    1860 
   Size:       1680     180
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     824    1860 
   Size:        824    1036
 
MscI TGG'CCA

Cuts at:      0     781    1860 
   Size:        781    1079
 
MslI CAynn'nnrTG

Cuts at:      0     316     787    1860 
   Size:        316     471    1073

  Fragments arranged by size: 

                1073     471     316
 
MspA1I CmG'CkG

Cuts at:      0     105     672    1860 
   Size:        105     567    1188

  Fragments arranged by size: 

                1188     567     105
 
NsiI A_TGCA'T

Cuts at:      0    1316    1860 
   Size:       1316     544
 
NspI r_CATG'y

Cuts at:      0     253     478    1860 
   Size:        253     225    1382

  Fragments arranged by size: 

                1382     253     225
 
PciI A'CATG_T

Cuts at:      0     249     474    1860 
   Size:        249     225    1386

  Fragments arranged by size: 

                1386     249     225
 
PfoI T'CCnGG_A

Cuts at:      0     149    1860 
   Size:        149    1711
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     350     382    1860 
   Size:        350      32    1478

  Fragments arranged by size: 

                1478     350      32
 
PstI C_TGCA'G

Cuts at:      0      47    1860 
   Size:         47    1813
 
PvuII CAG'CTG

Cuts at:      0     672    1860 
   Size:        672    1188
 
SacI G_AGCT'C

Cuts at:      0     737    1652    1860 
   Size:        737     915     208

  Fragments arranged by size: 

                 915     737     208
 
SapI GCTCTTCn'nnn_

Cuts at:      0     446    1860 
   Size:        446    1414
 
ScaI AGT'ACT

Cuts at:      0     910    1860 
   Size:        910     950
 
SfcI C'TryA_G

Cuts at:      0      43    1860 
   Size:         43    1817
 
SspI AAT'ATT

Cuts at:      0     642    1860 
   Size:        642    1218
 
StyI C'CwwG_G

Cuts at:      0     806    1860 
   Size:        806    1054
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     328    1860 
   Size:        328    1532
 
TatI w'GTAC_w

Cuts at:      0     908    1860 
   Size:        908     952
 
XmnI GAAnn'nnTTC

Cuts at:      0     463    1113    1860 
   Size:        463     650     747

  Fragments arranged by size: 

                 747     650     463
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII     AleI    AlwNI     ApoI
     AseI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BfrBI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI
    BstYI     BtsI     ClaI    CspCI   DraIII     EaeI     EarI     EciI
  EcoICRI  Eco57MI     FalI     FspI    HaeII    Hin4I  HindIII     KpnI
     MmeI     MscI     MslI   MspA1I     NsiI     NspI     PciI     PfoI
     PsrI     PstI    PvuII     SacI     SapI     ScaI     SfcI     SspI
     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AgeI     AhdI     AlfI     AloI
     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI     BbeI
    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmtI    BpuEI
     BsaI    BsaHI    BsiEI    BsiWI    BsmBI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     DraI     DrdI     EagI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI    FspAI   HincII     HpaI
     KasI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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