(Circular) (Six-base) MAPSORT of: pF1KE2341.seq Check: 4190 from: 1 to: 5321 pF1KE2341 5321 bp With 182 enzymes: SgfI * March 29, 2011 17:34 .. AccI GT'mk_AC Cuts at: 1060 2359 1060 Size: 1299 4022 Acc65I G'GTAC_C Cuts at: 2337 2337 Size: 5321 AclI AA'CG_TT Cuts at: 5161 5161 Size: 5321 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 548 746 766 1925 1957 2269 3146 3578 4325 Size: 198 20 1159 32 312 877 432 747 Cuts at: 4325 4593 548 Size: 268 1276 Fragments arranged by size: 1276 1159 877 747 432 312 268 198 32 20 AflIII A'CryG_T Cuts at: 2673 3777 4754 2673 Size: 1104 977 3240 Fragments arranged by size: 3240 1104 977 AgeI A'CCGG_T Cuts at: 3709 3709 Size: 5321 AlwNI CAG_nnn'CTG Cuts at: 2626 4193 2626 Size: 1567 3754 ApaLI G'TGCA_C Cuts at: 4091 4091 Size: 5321 ApoI r'AATT_y Cuts at: 410 633 1192 1286 1856 2325 4674 4930 410 Size: 223 559 94 570 469 2349 256 801 Fragments arranged by size: 2349 801 570 559 469 256 223 94 AseI AT'TA_AT Cuts at: 20 20 Size: 5321 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5321 AvaI C'yCGr_G Cuts at: 2341 2341 Size: 5321 BamHI G'GATC_C Cuts at: 1176 1414 2346 1176 Size: 238 932 4151 Fragments arranged by size: 4151 932 238 BanI G'GyrC_C Cuts at: 762 2337 2751 2999 3034 762 Size: 1575 414 248 35 3049 Fragments arranged by size: 3049 1575 414 248 35 BanII G_rGCy'C Cuts at: 115 2335 3365 115 Size: 2220 1030 2071 Fragments arranged by size: 2220 2071 1030 BbeI G_GCGC'C Cuts at: 2755 3003 2755 Size: 248 5073 BbsI GAAGACnn'nnnn_ Cuts at: 364 455 610 364 Size: 91 155 5075 Fragments arranged by size: 5075 155 91 BbvCI CC'TCA_GC Cuts at: 121 121 Size: 5321 BciVI GTATCCnnnnn_n' Cuts at: 979 3219 3980 5321 979 Size: 2240 761 1341 979 Fragments arranged by size: 2240 1341 979 761 BclI T'GATC_A Cuts at: 2841 2841 Size: 5321 BfrBI ATG'CAT Cuts at: 1909 1909 Size: 5321 BglII A'GATC_T Cuts at: 729 932 2836 4873 729 Size: 203 1904 2037 1177 Fragments arranged by size: 2037 1904 1177 203 BlpI GC'TnA_GC Cuts at: 2272 2433 2272 Size: 161 5160 Bme1580I G_kGCm'C Cuts at: 2946 3039 4095 2946 Size: 93 1056 4172 Fragments arranged by size: 4172 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 462 2533 2614 2949 5093 462 Size: 2071 81 335 2144 690 Fragments arranged by size: 2144 2071 690 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3688 3688 Size: 5321 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1103 1526 1907 1103 Size: 423 381 4517 Fragments arranged by size: 4517 423 381 Bpu10I CC'TnA_GC Cuts at: 121 264 121 Size: 143 5178 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2497 3376 3868 4166 2497 Size: 879 492 298 3652 Fragments arranged by size: 3652 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 5025 5025 Size: 5321 BsaAI yAC'GTr Cuts at: 2674 2674 Size: 5321 BsaBI GATnn'nnATC Cuts at: 6 2835 4872 6 Size: 2829 2037 455 Fragments arranged by size: 2829 2037 455 BsaHI Gr'CG_yC Cuts at: 2004 2752 3000 3702 5198 2004 Size: 748 248 702 1496 2127 Fragments arranged by size: 2127 1496 748 702 248 BsaWI w'CCGG_w Cuts at: 1417 2510 3031 3709 3983 4130 4541 1417 Size: 1093 521 678 274 147 411 2197 Fragments arranged by size: 2197 1093 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 197 227 335 365 669 699 860 890 1880 Size: 30 108 30 304 30 161 30 990 Cuts at: 1880 1910 197 Size: 30 3608 Fragments arranged by size: 3608 990 304 161 108 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 218 814 1040 218 Size: 596 226 4499 Fragments arranged by size: 4499 596 226 BseYI C'CCAG_C Cuts at: 851 2746 3504 4081 4577 851 Size: 1895 758 577 496 1595 Fragments arranged by size: 1895 1595 758 577 496 BsiEI CG_ry'CG Cuts at: 104 2863 2909 4117 104 Size: 2759 46 1208 1308 Fragments arranged by size: 2759 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 2335 3113 3303 4095 2335 Size: 778 190 792 3561 Fragments arranged by size: 3561 792 778 190 BsmI GAATG_Cn' Cuts at: 954 2230 954 Size: 1276 4045 Bsp1286I G_dGCh'C Cuts at: 115 2335 2946 3039 3113 3303 3365 4095 115 Size: 2220 611 93 74 190 62 730 1341 Fragments arranged by size: 2220 1341 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 4541 4541 Size: 5321 BspHI T'CATG_A Cuts at: 4497 4497 Size: 5321 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1669 2371 2585 2887 3268 1669 Size: 702 214 302 381 3722 Fragments arranged by size: 3722 702 381 302 214 BsrBI CCG'CTC Cuts at: 50 858 3613 3667 5151 5316 50 Size: 808 2755 54 1484 165 55 Fragments arranged by size: 2755 1484 808 165 55 54 BsrDI GCAATG_nn' Cuts at: 680 3233 680 Size: 2553 2768 BsrFI r'CCGG_y Cuts at: 151 3319 3500 3709 151 Size: 3168 181 209 1763 Fragments arranged by size: 3168 1763 209 181 BsrGI T'GTAC_A Cuts at: 1711 1711 Size: 5321 BssSI C'ACGA_G Cuts at: 787 3592 3950 787 Size: 2805 358 2158 Fragments arranged by size: 2805 2158 358 BstAPI GCAn_nnn'nTGC Cuts at: 1393 2152 2698 1393 Size: 759 546 4016 Fragments arranged by size: 4016 759 546 BstBI TT'CG_AA Cuts at: 3682 3682 Size: 5321 BstEII G'GTnAC_C Cuts at: 147 147 Size: 5321 BstXI CCAn_nnnn'nTGG Cuts at: 1885 1885 Size: 5321 BstYI r'GATC_y Cuts at: 1 729 932 1176 1414 1917 2346 2813 2836 Size: 728 203 244 238 503 429 467 23 Cuts at: 2836 3171 3417 4429 4515 4527 4873 1 Size: 335 246 1012 86 12 346 449 Fragments arranged by size: 1012 728 503 467 449 429 346 335 246 244 238 203 86 23 12 Bsu36I CC'TnA_GG Cuts at: 905 2027 905 Size: 1122 4199 BtgI C'CryG_G Cuts at: 107 107 Size: 5321 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3271 3452 3615 3271 Size: 181 163 4977 Fragments arranged by size: 4977 181 163 BtsI GCAGTG_nn' Cuts at: 320 2564 320 Size: 2244 3077 DraI TTT'AAA Cuts at: 2320 2320 Size: 5321 DrdI GACnn_nn'nnGTC Cuts at: 3027 3885 3027 Size: 858 4463 EaeI y'GGCC_r Cuts at: 317 585 1092 2906 3080 3471 3498 4843 317 Size: 268 507 1814 174 391 27 1345 795 Fragments arranged by size: 1814 1345 795 507 391 268 174 27 EagI C'GGCC_G Cuts at: 2906 2906 Size: 5321 EarI CTCTTCn'nnn_ Cuts at: 3344 3554 3344 Size: 210 5111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2093 3529 3839 3985 5131 2093 Size: 1436 310 146 1146 2283 Fragments arranged by size: 2283 1436 1146 310 146 EcoICRI GAG'CTC Cuts at: 2333 2333 Size: 5321 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 548 746 766 1103 1526 1907 1925 1957 2269 Size: 198 20 337 423 381 18 32 312 Cuts at: 2269 3146 3578 4325 4593 548 Size: 877 432 747 268 1276 Fragments arranged by size: 1276 877 747 432 423 381 337 312 268 198 32 20 18 EcoNI CCTnn'n_nnAGG Cuts at: 432 2147 432 Size: 1715 3606 EcoO109I rG'GnC_Cy Cuts at: 2140 2460 2140 Size: 320 5001 EcoRI G'AATT_C Cuts at: 410 1856 2325 410 Size: 1446 469 3406 Fragments arranged by size: 3406 1446 469 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2250 3340 2250 Size: 1090 4231 FspI TGC'GCA Cuts at: 3102 3102 Size: 5321 HaeII r_GCGC'y Cuts at: 2755 3003 4025 5004 2755 Size: 248 1022 979 3072 Fragments arranged by size: 3072 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1154 1186 1286 1318 1399 1431 1582 1614 1880 Size: 32 100 32 81 32 151 32 266 Cuts at: 1880 1912 2249 2281 3163 3193 3195 3225 1154 Size: 32 337 32 882 30 2 30 3250 Fragments arranged by size: 3250 882 337 266 151 100 81 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 399 2360 399 Size: 1961 3360 HindIII A'AGCT_T Cuts at: 1969 2212 1969 Size: 243 5078 KasI G'GCGC_C Cuts at: 2751 2999 2751 Size: 248 5073 KpnI G_GTAC'C Cuts at: 2341 2341 Size: 5321 MfeI C'AATT_G Cuts at: 644 644 Size: 5321 MluI A'CGCG_T Cuts at: 4754 4754 Size: 5321 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 804 3992 4176 804 Size: 3188 184 1949 Fragments arranged by size: 3188 1949 184 MscI TGG'CCA Cuts at: 319 587 1094 3082 319 Size: 268 507 1988 2558 Fragments arranged by size: 2558 1988 507 268 MslI CAynn'nnrTG Cuts at: 2197 3310 3437 2197 Size: 1113 127 4081 Fragments arranged by size: 4081 1113 127 MspA1I CmG'CkG Cuts at: 120 2432 2746 3106 4119 4364 120 Size: 2312 314 360 1013 245 1077 Fragments arranged by size: 2312 1077 1013 360 314 245 NaeI GCC'GGC Cuts at: 3502 3502 Size: 5321 NarI GG'CG_CC Cuts at: 2752 3000 2752 Size: 248 5073 NcoI C'CATG_G Cuts at: 107 107 Size: 5321 NdeI CA'TA_TG Cuts at: 1911 1911 Size: 5321 NgoMIV G'CCGG_C Cuts at: 3500 3500 Size: 5321 NsiI A_TGCA'T Cuts at: 1911 1911 Size: 5321 NspI r_CATG'y Cuts at: 2374 2874 3781 2374 Size: 500 907 3914 Fragments arranged by size: 3914 907 500 PciI A'CATG_T Cuts at: 3777 3777 Size: 5321 PflMI CCAn_nnn'nTGG Cuts at: 1933 1933 Size: 5321 PfoI T'CCnGG_A Cuts at: 1290 1812 1290 Size: 522 4799 PmeI GTTT'AAAC Cuts at: 2320 2320 Size: 5321 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 408 440 4460 4492 408 Size: 32 4020 32 1237 Fragments arranged by size: 4020 1237 32 32 PstI C_TGCA'G Cuts at: 2368 3053 2368 Size: 685 4636 PvuI CG_AT'CG Cuts at: 104 104 Size: 5321 PvuII CAG'CTG Cuts at: 2746 3106 2746 Size: 360 4961 RsrII CG'GwC_CG Cuts at: 3516 3516 Size: 5321 SacI G_AGCT'C Cuts at: 2335 2335 Size: 5321 SalI G'TCGA_C Cuts at: 2358 2358 Size: 5321 SapI GCTCTTCn'nnn_ Cuts at: 3344 3554 3344 Size: 210 5111 SbfI CC_TGCA'GG Cuts at: 2368 2368 Size: 5321 ScaI AGT'ACT Cuts at: 1336 4838 1336 Size: 3502 1819 SfcI C'TryA_G Cuts at: 33 1061 1550 2364 3049 4042 4233 33 Size: 1028 489 814 685 993 191 1121 Fragments arranged by size: 1121 1028 993 814 685 489 191 SfoI GGC'GCC Cuts at: 2753 3001 2753 Size: 248 5073 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5321 SgrAI Cr'CCGG_yG Cuts at: 151 151 Size: 5321 SmaI CCC'GGG Cuts at: 2343 2343 Size: 5321 SmlI C'TyrA_G Cuts at: 2476 3391 3883 4145 2476 Size: 915 492 262 3652 Fragments arranged by size: 3652 915 492 262 SpeI A'CTAG_T Cuts at: 67 1045 67 Size: 978 4343 SphI G_CATG'C Cuts at: 2374 2874 2374 Size: 500 4821 SspI AAT'ATT Cuts at: 2085 2085 Size: 5321 StyI C'CwwG_G Cuts at: 107 1152 1871 2136 2455 2809 107 Size: 1045 719 265 319 354 2619 Fragments arranged by size: 2619 1045 719 354 319 265 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 908 1258 2904 3037 3496 3705 908 Size: 350 1646 133 459 209 2524 Fragments arranged by size: 2524 1646 459 350 209 133 TatI w'GTAC_w Cuts at: 1334 1581 1711 4836 1334 Size: 247 130 3125 1819 Fragments arranged by size: 3125 1819 247 130 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 299 331 669 701 755 787 4838 4870 299 Size: 32 338 32 54 32 4051 32 750 Fragments arranged by size: 4051 750 338 54 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1537 1894 3118 1537 Size: 357 1224 3740 Fragments arranged by size: 3740 1224 357 XbaI T'CTAG_A Cuts at: 2352 2352 Size: 5321 XcmI CCAnnnn_n'nnnnTGG Cuts at: 979 2816 979 Size: 1837 3484 XmaI C'CCGG_G Cuts at: 2341 2341 Size: 5321 XmnI GAAnn'nnTTC Cuts at: 2230 2230 Size: 5321 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BcgI BglI BmgBI BmtI BsgI BsiWI BsmBI BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI NheI NotI NruI PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI StuI SwaI XhoI ZraI