Restriction map for pF1KB3769
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AhdI        
AocI        Aor51HI     AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvaI        AvrII       
BamHI       BbrPI       BbvCI       BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BseAI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrDI       
BsrGI       BssSI       BstBI       Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      Eam1105I    
EarI        Eco47III    Eco81I      EcoRI       EcoT22I     
FseI        HincII      HindII      HindIII     HpaI        
KpnI        Ksp632I     MaeI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PmaCI       PmeI        PmlI        
PshBI       PvuI        RsrII       SalI        SapI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI         1333  GTATAC               GT^[AC][GT]AC
AclI         1468  AACGTT               AA^CGTT
AcsI          232  AAATTC               [GA]^AATT[CT]
AflIII        626  ACGTGT               A^C[GA][CT]GT
AgeI         1492  ACCGGT               A^CCGGT
Alw44I        418  GTGCAC               G^TGCAC
ApaI          769  GGGCCC               GGGCC^C
ApaLI         418  GTGCAC               G^TGCAC
ApoI          232  AAATTC               [GA]^AATT[CT]
AspI         1609  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          179  TGGCCA               TGG^CCA
BbeI         1123  GGCGCC               GGCGC^C
BcgI          851  GCATCGAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1034  GTATCC               GTATCC(6/5)
BclI         1739  TGATCA               T^GATCA
BglI          149  GCCTCATCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         124  TACGTG               [CT]AC^GT[GA]
BsaI          331  GAGACC               GGTCTC(1/5)
BspHI        1235  TCATGA               T^CATGA
BsrBI        1576  GAGCGG               CCGCTC(-3/-3)
Bst1107I     1333  GTATAC               GTA^TAC
BstEII       1434  GGTTACC              G^GT[ACGT]ACC
BstPI        1434  GGTTACC              G^GT[ACGT]ACC
BstXI         119  CCAGCTACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1333  GTATAC               GTA^TAC
DrdI         1197  GACCCCGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          674  CGGCCG               C^GGCCG
EclXI         674  CGGCCG               C^GGCCG
Eco52I        674  CGGCCG               C^GGCCG
EcoNI         729  CCTGGCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1434  GGTTACC              G^GT[ACGT]ACC
EcoRV         357  GATATC               GAT^ATC
FbaI         1739  TGATCA               T^GATCA
KasI         1123  GGCGCC               G^GCGCC
KspI         1127  CCGCGG               CCGC^GG
MluNI         179  TGGCCA               TGG^CCA
MscI          179  TGGCCA               TGG^CCA
MseI         1466  TTAA                 T^TAA
MslI          375  CACGAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          676  GCCGGC               GCC^GGC
NarI         1123  GGCGCC               GG^CGCC
NcoI          365  CCATGG               C^CATGG
NgoMIV        676  GCCGGC               G^CCGGC
NruI         1605  TCGCGA               TCG^CGA
PflFI        1609  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1492  ACCGGT               A^CCGGT
Psp1406I     1468  AACGTT               AA^CGTT
PstI         1057  CTGCAG               CTGCA^G
PvuII        1223  CAGCTG               CAG^CTG
RcaI         1235  TCATGA               T^CATGA
SacII        1127  CCGCGG               CCGC^GG
SexAI         173  ACCAGGT              A^CC[AT]GGT
SfcI         1057  CTGCAG               C^T[GA][CT]AG
SfoI         1123  GGCGCC               GGC^GCC
Tru9I        1466  TTAA                 T^TAA
Tsp45I       1195  GTGAC                ^GT[GC]AC
Tsp509I       233  AATT                 ^AATT
Tth111I      1609  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AlwNI        1048  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1174  CAGGACCTG           
AviII         512  TGCGCA               TGC^GCA
              797  TGCGCA              
BbsI         1669  GAAGAC               GAAGAC(2/6)
               61  GTCTTC              
BpmI          949  CTGGAG               CTGGAG(16/14)
             1633  CTGGAG              
BpuAI        1669  GAAGAC               GAAGAC(2/6)
               61  GTCTTC              
BsaWI        1492  ACCGGT               [AT]^CCGG[AT]
             1556  ACCGGA              
BsmAI         331  GAGAC                GTCTC(1/5)
             1219  GAGAC               
BstAPI        800  GCACCGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1173  GCAGGACCTGC         
FspI          512  TGCGCA               TGC^GCA
              797  TGCGCA              
PshAI        1525  GACAAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1723  GACAAGTGTC          
TfiI         1398  GATTC                G^A[AT]TC
             1602  GAATC               
TspRI         248  CAGTG                CA[GC]TG[ACGT][ACGT]^
              268  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AspHI       AvaII       BanI        BanII       
BbvI        BcnI        BmyI        BsaHI       BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmFI       Bsp1286I    BspMI       BsrFI       
BsrI        BssHII      BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TaqI        TseI        TthHB8I     
XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com