Restriction map for pF1KB3769
 (Linear) (Six-base) MAPSORT of: KIBB3769.seq  Check: 9752  from: 1  to: 1917



KIBB3769.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:      0    1334    1917 
   Size:       1334     583
 
AclI AA'CG_TT

Cuts at:      0    1469    1917 
   Size:       1469     448
 
AflIII A'CryG_T

Cuts at:      0     626    1917 
   Size:        626    1291
 
AgeI A'CCGG_T

Cuts at:      0    1492    1917 
   Size:       1492     425
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1053    1179    1917 
   Size:       1053     126     738

  Fragments arranged by size: 

                1053     738     126
 
ApaI G_GGCC'C

Cuts at:      0     773    1917 
   Size:        773    1144
 
ApaLI G'TGCA_C

Cuts at:      0     418    1917 
   Size:        418    1499
 
ApoI r'AATT_y

Cuts at:      0     232    1917 
   Size:        232    1685
 
BanI G'GyrC_C

Cuts at:      0      37     487    1123    1917 
   Size:         37     450     636     794

  Fragments arranged by size: 

                 794     636     450      37
 
BanII G_rGCy'C

Cuts at:      0     503     530     773     835     923    1805    1917 
   Size:        503      27     243      62      88     882     112

  Fragments arranged by size: 

                 882     503     243     112      88      62      27
 
BbeI G_GCGC'C

Cuts at:      0    1127    1917 
   Size:       1127     790
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      54    1676    1917 
   Size:         54    1622     241

  Fragments arranged by size: 

                1622     241      54
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     840     874    1917 
   Size:        840      34    1043

  Fragments arranged by size: 

                1043     840      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1045    1917 
   Size:       1045     872
 
BclI T'GATC_A

Cuts at:      0    1739    1917 
   Size:       1739     178
 
BglI GCCn_nnn'nGGC

Cuts at:      0     155    1917 
   Size:        155    1762
 
Bme1580I G_kGCm'C

Cuts at:      0      40     422     773    1917 
   Size:         40     382     351    1144

  Fragments arranged by size: 

                1144     382     351      40
 
BmgBI CAC'GTC

Cuts at:      0     627    1194    1917 
   Size:        627     567     723

  Fragments arranged by size: 

                 723     627     567
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     378     879    1917 
   Size:        378     501    1038

  Fragments arranged by size: 

                1038     501     378
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     970    1654    1917 
   Size:        970     684     263

  Fragments arranged by size: 

                 970     684     263
 
Bpu10I CC'TnA_GC

Cuts at:      0     829    1917 
   Size:        829    1088
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     325    1917 
   Size:        325    1592
 
BsaAI yAC'GTr

Cuts at:      0     126    1917 
   Size:        126    1791
 
BsaHI Gr'CG_yC

Cuts at:      0     161     209     977    1124    1451    1917 
   Size:        161      48     768     147     327     466

  Fragments arranged by size: 

                 768     466     327     161     147      48
 
BsaWI w'CCGG_w

Cuts at:      0    1492    1556    1917 
   Size:       1492      64     361

  Fragments arranged by size: 

                1492     361      64
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     367     751     931    1917 
   Size:        367     384     180     986

  Fragments arranged by size: 

                 986     384     367     180
 
BseYI C'CCAG_C

Cuts at:      0     118    1209    1374    1917 
   Size:        118    1091     165     543

  Fragments arranged by size: 

                1091     543     165     118
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271    1153    1192    1348    1585    1917 
   Size:        271     882      39     156     237     332

  Fragments arranged by size: 

                 882     332     271     237     156      39
 
BsiEI CG_ry'CG

Cuts at:      0     677    1611    1755    1917 
   Size:        677     934     144     162

  Fragments arranged by size: 

                 934     677     162     144
 
BsiHKAI G_wGCw'C

Cuts at:      0     422     596     835     923    1492    1805    1917 
   Size:        422     174     239      88     569     313     112

  Fragments arranged by size: 

                 569     422     313     239     174     112      88
 
Bsp1286I G_dGCh'C

Cuts at:      0      40     422     503     530     596     773     835     923 
   Size:         40     382      81      27      66     177      62      88

Cuts at:    923    1492    1805    1917 
   Size:        569     313     112

  Fragments arranged by size: 

                 569     382     313     177     112      88      81      66
                  62      40      27
 
BspHI T'CATG_A

Cuts at:      0    1235    1917 
   Size:       1235     682
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1187    1320    1379    1917 
   Size:       1187     133      59     538

  Fragments arranged by size: 

                1187     538     133      59
 
BsrBI CCG'CTC

Cuts at:      0    1578    1917 
   Size:       1578     339
 
BsrFI r'CCGG_y

Cuts at:      0     676     954    1492    1917 
   Size:        676     278     538     425

  Fragments arranged by size: 

                 676     538     425     278
 
BssHII G'CGCG_C

Cuts at:      0     780     813     903    1917 
   Size:        780      33      90    1014

  Fragments arranged by size: 

                1014     780      90      33
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     806    1179    1917 
   Size:        806     373     738

  Fragments arranged by size: 

                 806     738     373
 
BstEII G'GTnAC_C

Cuts at:      0    1434    1917 
   Size:       1434     483
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     126    1917 
   Size:        126    1791
 
BstYI r'GATC_y

Cuts at:      0     998    1043    1715    1917 
   Size:        998      45     672     202

  Fragments arranged by size: 

                 998     672     202      45
 
BstZ17I GTA'TAC

Cuts at:      0    1335    1917 
   Size:       1335     582
 
BtgI C'CryG_G

Cuts at:      0       9     365     530     671    1127    1917 
   Size:          9     356     165     141     456     790

  Fragments arranged by size: 

                 790     456     356     165     141       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      72     528    1917 
   Size:         72     456    1389

  Fragments arranged by size: 

                1389     456      72
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1203    1917 
   Size:       1203     714
 
EaeI y'GGCC_r

Cuts at:      0     179     271     539     674    1139    1619    1917 
   Size:        179      92     268     135     465     480     298

  Fragments arranged by size: 

                 480     465     298     268     179     135      92
 
EagI C'GGCC_G

Cuts at:      0     674    1917 
   Size:        674    1243
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     748    1588    1917 
   Size:        748     840     329

  Fragments arranged by size: 

                 840     748     329
 
EcoICRI GAG'CTC

Cuts at:      0     833     921    1803    1917 
   Size:        833      88     882     114

  Fragments arranged by size: 

                 882     833     114      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     970    1654    1917 
   Size:        970     684     263

  Fragments arranged by size: 

                 970     684     263
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     733    1917 
   Size:        733    1184
 
EcoO109I rG'GnC_Cy

Cuts at:      0     944    1176    1419    1894    1917 
   Size:        944     232     243     475      23

  Fragments arranged by size: 

                 944     475     243     232      23
 
EcoRV GAT'ATC

Cuts at:      0     359    1917 
   Size:        359    1558
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     360    1917 
   Size:        360    1557
 
FspI TGC'GCA

Cuts at:      0     514     799    1917 
   Size:        514     285    1118

  Fragments arranged by size: 

                1118     514     285
 
HaeII r_GCGC'y

Cuts at:      0     150     468     970    1127    1917 
   Size:        150     318     502     157     790

  Fragments arranged by size: 

                 790     502     318     157     150
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     343     375     652     684    1886    1917 
   Size:        343      32     277      32    1202      31

  Fragments arranged by size: 

                1202     343     277      32      32      31
 
KasI G'GCGC_C

Cuts at:      0    1123    1917 
   Size:       1123     794
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     236    1917 
   Size:         90     146    1681

  Fragments arranged by size: 

                1681     146      90
 
MscI TGG'CCA

Cuts at:      0     181    1917 
   Size:        181    1736
 
MslI CAynn'nnrTG

Cuts at:      0     379    1917 
   Size:        379    1538
 
MspA1I CmG'CkG

Cuts at:      0    1129    1225    1865    1917 
   Size:       1129      96     640      52

  Fragments arranged by size: 

                1129     640      96      52
 
NaeI GCC'GGC

Cuts at:      0     678    1917 
   Size:        678    1239
 
NarI GG'CG_CC

Cuts at:      0    1124    1917 
   Size:       1124     793
 
NcoI C'CATG_G

Cuts at:      0     365    1917 
   Size:        365    1552
 
NgoMIV G'CCGG_C

Cuts at:      0     676    1917 
   Size:        676    1241
 
NruI TCG'CGA

Cuts at:      0    1607    1917 
   Size:       1607     310
 
PfoI T'CCnGG_A

Cuts at:      0    1001    1917 
   Size:       1001     916
 
PpuMI rG'GwC_Cy

Cuts at:      0     944    1176    1419    1894    1917 
   Size:        944     232     243     475      23

  Fragments arranged by size: 

                 944     475     243     232      23
 
PshAI GACnn'nnGTC

Cuts at:      0    1529    1727    1917 
   Size:       1529     198     190

  Fragments arranged by size: 

                1529     198     190
 
PspOMI G'GGCC_C

Cuts at:      0     769    1917 
   Size:        769    1148
 
PstI C_TGCA'G

Cuts at:      0    1061    1917 
   Size:       1061     856
 
PvuII CAG'CTG

Cuts at:      0    1225    1917 
   Size:       1225     692
 
SacI G_AGCT'C

Cuts at:      0     835     923    1805    1917 
   Size:        835      88     882     112

  Fragments arranged by size: 

                 882     835     112      88
 
SacII CC_GC'GG

Cuts at:      0    1130    1917 
   Size:       1130     787
 
SanDI GG'GwC_CC

Cuts at:      0     944    1419    1894    1917 
   Size:        944     475     475      23

  Fragments arranged by size: 

                 944     475     475      23
 
SexAI A'CCwGG_T

Cuts at:      0     173    1917 
   Size:        173    1744
 
SfcI C'TryA_G

Cuts at:      0    1057    1917 
   Size:       1057     860
 
SfoI GGC'GCC

Cuts at:      0    1125    1917 
   Size:       1125     792
 
StyI C'CwwG_G

Cuts at:      0     365     476    1358    1842    1917 
   Size:        365     111     882     484      75

  Fragments arranged by size: 

                 882     484     365     111      75
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     700    1625    1769    1917 
   Size:        700     925     144     148

  Fragments arranged by size: 

                 925     700     148     144
 
TatI w'GTAC_w

Cuts at:      0     320    1917 
   Size:        320    1597
 
Tth111I GACn'n_nGTC

Cuts at:      0    1612    1917 
   Size:       1612     305
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     174    1629    1849    1908    1917 
   Size:        174    1455     220      59       9

  Fragments arranged by size: 

                1455     220     174      59       9
 

 Enzymes that do cut:

     AccI     AclI   AflIII     AgeI    AlwNI     ApaI    ApaLI     ApoI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI     BglI
 Bme1580I    BmgBI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrBI    BsrFI   BssHII   BstAPI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FspI    HaeII    Hin4I
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NruI     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SacI    SacII    SanDI    SexAI     SfcI     SfoI     StyI    TaqII
     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AfeI    AflII     AhdI     AleI
     AlfI     AloI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BbvCI    BfrBI    BglII     BlpI     BmrI     BmtI    BpuEI
    BsaBI    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrGI
    BssSI    BstBI   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     EarI    EcoRI     FseI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     NdeI     NheI     NotI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII     SalI     SapI     SbfI     ScaI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI     XbaI     XhoI     XmaI     XmnI
     ZraI
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