Restriction map for pF1KB6876
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BcnI        BfaI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        ClaI        CpoI        DdeI        
DraI        DraIII      DrdI        Eam1105I    EarI        
Eco47III    Eco81I      EcoO65I     EcoRV       EcoT22I     
FokI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        Ksp632I     MaeI        
MamI        MfeI        MluI        MroI        MseI        
MslI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tsp45I      Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I         92  GGTACC               G^GTACC
AccII         282  CGCG                 CG^CG
AlwNI         298  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           20  GGGCCC               GGGCC^C
Asp700I       507  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I        92  GGTACC               G^GTACC
AspHI          84  GTGCTC               G[AT]GC[AT]^C
AvaI          403  CCCGAG               C^[CT]CG[GA]G
AvaII         344  GGACC                G^G[AT]CC
BalI          530  TGGCCA               TGG^CCA
BanII          20  GGGCCC               G[GA]GC[CT]^C
BclI          395  TGATCA               T^GATCA
BglII         461  AGATCT               A^GATCT
BsgI          268  CTGCAC               GTGCAG(16/14)
BsiEI         123  CGGCCG               CG[GA][CT]^CG
BsiHKAI        84  GTGCTC               G[AT]GC[AT]^C
BsoBI         403  CCCGAG               C^[CT]CG[GA]G
BsrFI         121  ACCGGC               [GA]^CCGG[CT]
BstUI         282  CGCG                 CG^CG
Cfr10I        121  ACCGGC               [GA]^CCGG[CT]
DsaI          281  CCGCGG               C^C[GA][CT]GG
EagI          123  CGGCCG               C^GGCCG
EclXI         123  CGGCCG               C^GGCCG
Eco52I        123  CGGCCG               C^GGCCG
EcoNI          24  CCTGTCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         511  GAATTC               G^AATTC
FauI          283  GCGGG                CCCGC(4/6)
FbaI          395  TGATCA               T^GATCA
HinfI         220  GATTC                G^A[ACGT]TC
HphI          131  TCACC                GGTGA(8/7)
KpnI           92  GGTACC               GGTAC^C
KspI          281  CCGCGG               CCGC^GG
MaeIII        338  GTAAC                ^GT[ACGT]AC
MluNI         530  TGGCCA               TGG^CCA
MscI          530  TGGCCA               TGG^CCA
MvnI          282  CGCG                 CG^CG
MwoI          441  GCCGGAAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI          201  GCATGT               [GA]CATG^[CT]
PflMI         297  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI           49  CTGCAG               CTGCA^G
PvuII          52  CAGCTG               CAG^CTG
SacII         281  CCGCGG               CCGC^GG
ScaI          470  AGTACT               AGT^ACT
SfcI           49  CTGCAG               C^T[GA][CT]AG
StuI          188  AGGCCT               AGG^CCT
TfiI          220  GATTC                G^A[AT]TC
TspRI         527  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        297  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          507  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI          511  GAATTC               [GA]^AATT[CT]
              542  AAATTC              
AlwI           36  GATCC                GGATC(4/5)
              555  GATCC               
ApoI          511  GAATTC               [GA]^AATT[CT]
              542  AAATTC              
BanI           92  GGTACC               G^G[CT][GA]CC
              235  GGCACC              
BmyI           20  GGGCCC               G[AGT]GC[ACT]^C
               84  GTGCTC              
BsiYI          24  CCTGTCCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              297  CCAGAAGCTGG         
BslI           24  CCTGTCCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              297  CCAGAAGCTGG         
Bsp1286I       20  GGGCCC               G[AGT]GC[ACT]^C
               84  GTGCTC              
BstYI          35  AGATCC               [GA]^GATC[CT]
              461  AGATCT              
Cac8I         283  GCGGGC               GC[ACGT]^[ACGT]GC
              450  GCGAGC              
DraII          19  GGGGCCC              [GA]G^G[ACGT]CC[CT]
               20  GGGCCCT             
EaeI          123  CGGCCG               [CT]^GGCC[GA]
              530  TGGCCA              
EcoO109I       19  GGGGCCC              [GA]G^G[ACGT]CC[CT]
               20  GGGCCCT             
EcoT14I        29  CCAAGG               C^C[AT][AT]GG
              185  CCAAGG              
HapII         122  CCGG                 C^CGG
              442  CCGG                
HpaII         122  CCGG                 C^CGG
              442  CCGG                
MflI           35  AGATCC               [GA]^GATC[CT]
              461  AGATCT              
MspA1I         52  CAGCTG               C[AC]G^C[GT]G
              281  CCGCGG              
MspI          122  CCGG                 C^CGG
              442  CCGG                
SfaNI         128  GCATC                GCATC(5/9)
              152  GCATC               
StyI           29  CCAAGG               C^C[AT][AT]GG
              185  CCAAGG              
TaqI          218  TCGA                 T^CGA
              230  TCGA                
Tsp509I       512  AATT                 ^AATT
              543  AATT                
TthHB8I       218  TCGA                 T^CGA
              230  TCGA                
XcmI           29  CCAAGGAGATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              293  CCAACCAGAAGCTGG     
XhoII          35  AGATCC               [GA]^GATC[CT]
              461  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BpmI        
BsaJI       BseRI       BsmFI       BsrI        BssKI       
BstNI       Cfr13I      DpnII       EcoRII      Fnu4HI      
HaeIII      ItaI        MaeII       MboI        MboII       
MnlI        MvaI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       TseI        
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