Restriction map for pF1KB6876
 (Linear) (Six-base) MAPSORT of: KIBB6876.seq  Check: 3802  from: 1  to: 600



KIBB6876.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

Acc65I G'GTAC_C

Cuts at:      0      92     600 
   Size:         92     508
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     600 
   Size:        127     473
 
AlwNI CAG_nnn'CTG

Cuts at:      0     303     600 
   Size:        303     297
 
ApaI G_GGCC'C

Cuts at:      0      24     600 
   Size:         24     576
 
ApoI r'AATT_y

Cuts at:      0     511     542     600 
   Size:        511      31      58

  Fragments arranged by size: 

                 511      58      31
 
AvaI C'yCGr_G

Cuts at:      0     403     600 
   Size:        403     197
 
BanI G'GyrC_C

Cuts at:      0      92     235     600 
   Size:         92     143     365

  Fragments arranged by size: 

                 365     143      92
 
BanII G_rGCy'C

Cuts at:      0      24     600 
   Size:         24     576
 
BclI T'GATC_A

Cuts at:      0     395     600 
   Size:        395     205
 
BglII A'GATC_T

Cuts at:      0     461     600 
   Size:        461     139
 
Bme1580I G_kGCm'C

Cuts at:      0      24     600 
   Size:         24     576
 
BmgBI CAC'GTC

Cuts at:      0     330     411     600 
   Size:        330      81     189

  Fragments arranged by size: 

                 330     189      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     102     600 
   Size:        102     498
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     325     388     600 
   Size:         61     264      63     212

  Fragments arranged by size: 

                 264     212      63      61
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      67      97     600 
   Size:         67      30     503

  Fragments arranged by size: 

                 503      67      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58      88      91     600 
   Size:         58      30       3     509

  Fragments arranged by size: 

                 509      58      30       3
 
BseYI C'CCAG_C

Cuts at:      0     134     197     600 
   Size:        134      63     403

  Fragments arranged by size: 

                 403     134      63
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     600 
   Size:        253     347
 
BsiEI CG_ry'CG

Cuts at:      0     126     600 
   Size:        126     474
 
BsiHKAI G_wGCw'C

Cuts at:      0      88     600 
   Size:         88     512
 
Bsp1286I G_dGCh'C

Cuts at:      0      24      88     600 
   Size:         24      64     512

  Fragments arranged by size: 

                 512      64      24
 
BsrFI r'CCGG_y

Cuts at:      0     121     600 
   Size:        121     479
 
BstYI r'GATC_y

Cuts at:      0      35     461     600 
   Size:         35     426     139

  Fragments arranged by size: 

                 426     139      35
 
BtgI C'CryG_G

Cuts at:      0     281     600 
   Size:        281     319
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     246     600 
   Size:        246     354
 
EaeI y'GGCC_r

Cuts at:      0     123     530     600 
   Size:        123     407      70

  Fragments arranged by size: 

                 407     123      70
 
EagI C'GGCC_G

Cuts at:      0     123     600 
   Size:        123     477
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     127     325     388     600 
   Size:         61      66     198      63     212

  Fragments arranged by size: 

                 212     198      66      63      61
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      28     600 
   Size:         28     572
 
EcoO109I rG'GnC_Cy

Cuts at:      0      20      21     600 
   Size:         20       1     579

  Fragments arranged by size: 

                 579      20       1
 
EcoRI G'AATT_C

Cuts at:      0     511     600 
   Size:        511      89
 
KpnI G_GTAC'C

Cuts at:      0      96     600 
   Size:         96     504
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     248     600 
   Size:        248     352
 
MscI TGG'CCA

Cuts at:      0     532     600 
   Size:        532      68
 
MspA1I CmG'CkG

Cuts at:      0      54     283     600 
   Size:         54     229     317

  Fragments arranged by size: 

                 317     229      54
 
NspI r_CATG'y

Cuts at:      0     205     600 
   Size:        205     395
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     303     600 
   Size:        303     297
 
PfoI T'CCnGG_A

Cuts at:      0      38     600 
   Size:         38     562
 
PspOMI G'GGCC_C

Cuts at:      0      20     600 
   Size:         20     580
 
PstI C_TGCA'G

Cuts at:      0      53     600 
   Size:         53     547
 
PvuII CAG'CTG

Cuts at:      0      54     600 
   Size:         54     546
 
SacII CC_GC'GG

Cuts at:      0     284     600 
   Size:        284     316
 
ScaI AGT'ACT

Cuts at:      0     472     600 
   Size:        472     128
 
SfcI C'TryA_G

Cuts at:      0      49     600 
   Size:         49     551
 
StuI AGG'CCT

Cuts at:      0     190     600 
   Size:        190     410
 
StyI C'CwwG_G

Cuts at:      0      29     185     600 
   Size:         29     156     415

  Fragments arranged by size: 

                 415     156      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     148     600 
   Size:        148     452
 
TatI w'GTAC_w

Cuts at:      0     470     600 
   Size:        470     130
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      67      99     600 
   Size:         67      32     501

  Fragments arranged by size: 

                 501      67      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      36     300     600 
   Size:         36     264     300

  Fragments arranged by size: 

                 300     264      36
 
XmnI GAAnn'nnTTC

Cuts at:      0     511     600 
   Size:        511      89
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII
     BclI    BglII Bme1580I    BmgBI     BplI     BpmI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BsrFI    BstYI     BtgI
    BtgZI     EaeI     EagI  Eco57MI    EcoNI EcoO109I    EcoRI     KpnI
     MmeI     MscI   MspA1I     NspI    PflMI     PfoI   PspOMI     PstI
    PvuII    SacII     ScaI     SfcI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI     BlpI     BmrI     BmtI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI     EciI  EcoICRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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