Restriction map for pF1KB5749
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AccIII      AclI        AflII       AflIII      AgeI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        AvrII       BbeI        
BbrPI       BcgI        BfrI        BglI        BlnI        
BsaWI       BseAI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoT22I     FseI        HgaI        HincII      
HindII      HindIII     HpaI        KasI        MfeI        
MluI        MroI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SpeI        SphI        SspBI       StuI        SwaI        
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII         652  GACGTC               GACGT^C
Acc65I          7  GGTACC               G^GTACC
AccII         338  CGCG                 CG^CG
AcyI          652  GACGTC               G[GA]^CG[CT]C
AhdI         1234  GACAAAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1057  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      2232  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I         7  GGTACC               G^GTACC
AspEI        1234  GACAAAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1088  CCCGAG               C^[CT]CG[GA]G
BamHI        1467  GGATCC               G^GATCC
BanI            7  GGTACC               G^G[CT][GA]CC
BciVI         917  GTATCC               GTATCC(6/5)
BclI          116  TGATCA               T^GATCA
BglII        1487  AGATCT               A^GATCT
BlpI          814  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      814  GCTCAGC              GC^T[ACGT]AGC
BsaAI         295  TACGTA               [CT]AC^GT[GA]
BsaBI        2380  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         652  GACGTC               G[GA]^CG[CT]C
BsmBI         330  CGTCTC               CGTCTC(1/5)
BsoBI        1088  CCCGAG               C^[CT]CG[GA]G
BspHI        1700  TCATGA               T^CATGA
BsrBI         642  CCGCTC               CCGCTC(-3/-3)
BstEII        694  GGTGACC              G^GT[ACGT]ACC
BstPI         694  GGTGACC              G^GT[ACGT]ACC
BstUI         338  CGCG                 CG^CG
CelII         814  GCTCAGC              GC^T[ACGT]AGC
Eam1105I     1234  GACAAAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1743  CCTGACACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       694  GGTGACC              G^GT[ACGT]ACC
FbaI          116  TGATCA               T^GATCA
HaeII        1169  AGCGCC               [GA]GCGC^[CT]
Hin1I         652  GACGTC               G[GA]^CG[CT]C
KpnI            7  GGTACC               GGTAC^C
KspI          337  CCGCGG               CCGC^GG
MamI         2380  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI          338  CGCG                 CG^CG
NaeI          628  GCCGGC               GCC^GGC
NgoMIV        628  GCCGGC               G^CCGGC
PflMI        2202  CCAGAAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         760  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI         1700  TCATGA               T^CATGA
SacI         1809  GAGCTC               GAGCT^C
SacII         337  CCGCGG               CCGC^GG
SbfI         1914  CCTGCAGG             CCTGCA^GG
SnaBI         295  TACGTA               TAC^GTA
Sse8387I     1914  CCTGCAGG             CCTGCA^GG
SspI         1018  AATATT               AAT^ATT
Van91I       2202  CCAGAAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         2232  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          574  GTCTAC               GT^[AC][GT]AC
             1363  GTCTAC              
AcsI           89  GAATTT               [GA]^AATT[CT]
             1228  GAATTT              
ApoI           89  GAATTT               [GA]^AATT[CT]
             1228  GAATTT              
AspHI        1523  GAGCAC               G[AT]GC[AT]^C
             1809  GAGCTC              
AspI          931  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1495  GACCATGTC           
BbvCI         360  CCTCAGC              CCTCAGC(-5/-2)
              832  GCTGAGG             
BfaI          406  CTAG                 C^TAG
              985  CTAG                
BsgI         1082  CTGCAC               GTGCAG(16/14)
             1691  GTGCAG              
BsiHKAI      1523  GAGCAC               G[AT]GC[AT]^C
             1809  GAGCTC              
BsrDI        1296  CATTGC               GCAATG(2/0)
             1767  CATTGC              
DraII         620  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              760  GGGACCT             
DrdI         1746  GACACAGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2383  GACAATCAGGTC        
EcoO109I      620  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              760  GGGACCT             
EcoRV         880  GATATC               GAT^ATC
             1573  GATATC              
MaeI          406  CTAG                 C^TAG
              985  CTAG                
NcoI          921  CCATGG               C^CATGG
             1190  CCATGG              
PflFI         931  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1495  GACCATGTC           
PstI         1335  CTGCAG               CTGCA^G
             1915  CTGCAG              
SapI         2329  GAAGAGC              GCTCTTC(1/4)
             1443  GCTCTTC             
SmlI          550  CTTGAG               C^T[CT][GA]AG
             2153  CTCAAG              
TaqI         1287  TCGA                 T^CGA
             1866  TCGA                
Tsp509I        90  AATT                 ^AATT
             1229  AATT                
Tth111I       931  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1495  GACCATGTC           
TthHB8I      1287  TCGA                 T^CGA
             1866  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
AviII       BalI        BanII       BbsI        BbvI        
BcnI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DsaI        EaeI        EarI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
FspI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        Tru9I       TseI        
Tsp45I      TspRI       XhoII       
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