Restriction map for pF1KE2363
 (Linear) (Six-base) MAPSORT of: KIEE2363.seq  Check: 188  from: 1  to: 1551



KIEE2363.orf

 With 182 enzymes: SgfI * 

                        July 21, 2011 14:40  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     724    1438    1551 
   Size:        724     714     113

  Fragments arranged by size: 

                 724     714     113
 
AccI GT'mk_AC

Cuts at:      0     350    1551 
   Size:        350    1201
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227    1551 
   Size:        227    1324
 
AflII C'TTAA_G

Cuts at:      0    1495    1551 
   Size:       1495      56
 
AgeI A'CCGG_T

Cuts at:      0     781    1551 
   Size:        781     770
 
AleI CACnn'nnGTG

Cuts at:      0     793    1551 
   Size:        793     758
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1269    1551 
   Size:       1269     282
 
AvaI C'yCGr_G

Cuts at:      0     248     364    1006    1349    1551 
   Size:        248     116     642     343     202

  Fragments arranged by size: 

                 642     343     248     202     116
 
AvrII C'CTAG_G

Cuts at:      0    1110    1551 
   Size:       1110     441
 
BanI G'GyrC_C

Cuts at:      0      15     278    1551 
   Size:         15     263    1273

  Fragments arranged by size: 

                1273     263      15
 
BanII G_rGCy'C

Cuts at:      0     524     871    1551 
   Size:        524     347     680

  Fragments arranged by size: 

                 680     524     347
 
BbeI G_GCGC'C

Cuts at:      0     282    1551 
   Size:        282    1269
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     160    1551 
   Size:        160    1391
 
BglI GCCn_nnn'nGGC

Cuts at:      0      38    1551 
   Size:         38    1513
 
BglII A'GATC_T

Cuts at:      0    1247    1551 
   Size:       1247     304
 
BlpI GC'TnA_GC

Cuts at:      0      28    1551 
   Size:         28    1523
 
Bme1580I G_kGCm'C

Cuts at:      0     200    1551 
   Size:        200    1351
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     871    1372    1551 
   Size:         19     852     501     179

  Fragments arranged by size: 

                 852     501     179      19
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820    1551 
   Size:        820     731
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     361    1551 
   Size:        361    1190
 
BsaAI yAC'GTr

Cuts at:      0     201    1551 
   Size:        201    1350
 
BsaBI GATnn'nnATC

Cuts at:      0     227    1551 
   Size:        227    1324
 
BsaHI Gr'CG_yC

Cuts at:      0     279    1551 
   Size:        279    1272
 
BsaWI w'CCGG_w

Cuts at:      0     781    1551 
   Size:        781     770
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      48    1141    1258    1306    1551 
   Size:         48    1093     117      48     245

  Fragments arranged by size: 

                1093     245     117      48      48
 
BseYI C'CCAG_C

Cuts at:      0     452     749     855     871    1067    1479    1551 
   Size:        452     297     106      16     196     412      72

  Fragments arranged by size: 

                 452     412     297     196     106      72      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1237    1282    1551 
   Size:       1237      45     269

  Fragments arranged by size: 

                1237     269      45
 
Bsp1286I G_dGCh'C

Cuts at:      0     200     524     871    1551 
   Size:        200     324     347     680

  Fragments arranged by size: 

                 680     347     324     200
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     724    1438    1551 
   Size:        724     714     113

  Fragments arranged by size: 

                 724     714     113
 
BsrBI CCG'CTC

Cuts at:      0     300     339    1551 
   Size:        300      39    1212

  Fragments arranged by size: 

                1212     300      39
 
BsrFI r'CCGG_y

Cuts at:      0     305     781    1551 
   Size:        305     476     770

  Fragments arranged by size: 

                 770     476     305
 
BstEII G'GTnAC_C

Cuts at:      0     211    1521    1551 
   Size:        211    1310      30

  Fragments arranged by size: 

                1310     211      30
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     486     795    1551 
   Size:        486     309     756

  Fragments arranged by size: 

                 756     486     309
 
BstYI r'GATC_y

Cuts at:      0    1247    1353    1551 
   Size:       1247     106     198

  Fragments arranged by size: 

                1247     198     106
 
Bsu36I CC'TnA_GG

Cuts at:      0    1079    1551 
   Size:       1079     472
 
BtgI C'CryG_G

Cuts at:      0     274     485     550     908    1016    1551 
   Size:        274     211      65     358     108     535

  Fragments arranged by size: 

                 535     358     274     211     108      65
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     729    1551 
   Size:        729     822
 
BtsI GCAGTG_nn'

Cuts at:      0     922    1551 
   Size:        922     629
 
EaeI y'GGCC_r

Cuts at:      0     492     537     740     884    1128    1551 
   Size:        492      45     203     144     244     423

  Fragments arranged by size: 

                 492     423     244     203     144      45
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     250    1175    1551 
   Size:        250     925     376

  Fragments arranged by size: 

                 925     376     250
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     227     871    1372    1551 
   Size:         19     208     644     501     179

  Fragments arranged by size: 

                 644     501     208     179      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     673    1551 
   Size:        673     878
 
EcoO109I rG'GnC_Cy

Cuts at:      0     269    1075    1551 
   Size:        269     806     476

  Fragments arranged by size: 

                 806     476     269
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     174    1551 
   Size:        174    1377
 
HaeII r_GCGC'y

Cuts at:      0     282    1551 
   Size:        282    1269
 
HincII GTy'rAC

Cuts at:      0     171     765    1551 
   Size:        171     594     786

  Fragments arranged by size: 

                 786     594     171
 
KasI G'GCGC_C

Cuts at:      0     278    1551 
   Size:        278    1273
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     559     854    1551 
   Size:        559     295     697

  Fragments arranged by size: 

                 697     559     295
 
MscI TGG'CCA

Cuts at:      0     494     539    1130    1551 
   Size:        494      45     591     421

  Fragments arranged by size: 

                 591     494     421      45
 
MslI CAynn'nnrTG

Cuts at:      0     430     484     758     793    1551 
   Size:        430      54     274      35     758

  Fragments arranged by size: 

                 758     430     274      54      35
 
MspA1I CmG'CkG

Cuts at:      0     114     820    1551 
   Size:        114     706     731

  Fragments arranged by size: 

                 731     706     114
 
NaeI GCC'GGC

Cuts at:      0     307    1551 
   Size:        307    1244
 
NarI GG'CG_CC

Cuts at:      0     279    1551 
   Size:        279    1272
 
NcoI C'CATG_G

Cuts at:      0     274     485    1551 
   Size:        274     211    1066

  Fragments arranged by size: 

                1066     274     211
 
NgoMIV G'CCGG_C

Cuts at:      0     305    1551 
   Size:        305    1246
 
NspI r_CATG'y

Cuts at:      0     907    1551 
   Size:        907     644
 
PasI CC'CwG_GG

Cuts at:      0     694    1551 
   Size:        694     857
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     756    1551 
   Size:        756     795
 
PfoI T'CCnGG_A

Cuts at:      0     967    1551 
   Size:        967     584
 
PmlI CAC'GTG

Cuts at:      0     201    1551 
   Size:        201    1350
 
PpuMI rG'GwC_Cy

Cuts at:      0     269    1551 
   Size:        269    1282
 
PvuII CAG'CTG

Cuts at:      0     114     820    1551 
   Size:        114     706     731

  Fragments arranged by size: 

                 731     706     114
 
SanDI GG'GwC_CC

Cuts at:      0     269    1551 
   Size:        269    1282
 
SexAI A'CCwGG_T

Cuts at:      0    1318    1551 
   Size:       1318     233
 
SfoI GGC'GCC

Cuts at:      0     280    1551 
   Size:        280    1271
 
SmaI CCC'GGG

Cuts at:      0     250    1351    1551 
   Size:        250    1101     200

  Fragments arranged by size: 

                1101     250     200
 
SmlI C'TyrA_G

Cuts at:      0     364     835    1006    1495    1551 
   Size:        364     471     171     489      56

  Fragments arranged by size: 

                 489     471     364     171      56
 
SphI G_CATG'C

Cuts at:      0     907    1551 
   Size:        907     644
 
StuI AGG'CCT

Cuts at:      0    1501    1551 
   Size:       1501      50
 
StyI C'CwwG_G

Cuts at:      0     274     371     407     485     563    1110    1551 
   Size:        274      97      36      78      78     547     441

  Fragments arranged by size: 

                 547     441     274      97      78      78      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     847    1081    1393    1471    1551 
   Size:        847     234     312      78      80

  Fragments arranged by size: 

                 847     312     234      80      78
 
TatI w'GTAC_w

Cuts at:      0    1370    1551 
   Size:       1370     181
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     817     879    1326    1441    1551 
   Size:        817      62     447     115     110

  Fragments arranged by size: 

                 817     447     115     110      62
 
XhoI C'TCGA_G

Cuts at:      0     364    1006    1551 
   Size:        364     642     545

  Fragments arranged by size: 

                 642     545     364
 
XmaI C'CCGG_G

Cuts at:      0     248    1349    1551 
   Size:        248    1101     202

  Fragments arranged by size: 

                1101     248     202
 
XmnI GAAnn'nnTTC

Cuts at:      0     157     388    1551 
   Size:        157     231    1163

  Fragments arranged by size: 

                1163     231     157
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII     AgeI     AleI    AlwNI     AvaI
    AvrII     BanI    BanII     BbeI     BbsI     BglI    BglII     BlpI
 Bme1580I     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI Bsp1286I    BspMI    BsrBI    BsrFI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EciI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII   HincII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI    PvuII    SanDI    SexAI
     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI
     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AhdI     AlfI     AloI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I  HindIII
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI     SfcI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     ZraI
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