Restriction map for pF1KB5748
 (Linear) (Six-base) MAPSORT of: KIBB5748.seq  Check: 3533  from: 1  to: 2238



KIBB5748.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     898    2238 
   Size:        898    1340
 
AccI GT'mk_AC

Cuts at:      0     347     353    2238 
   Size:        347       6    1885

  Fragments arranged by size: 

                1885     347       6
 
Acc65I G'GTAC_C

Cuts at:      0     809    1772    2238 
   Size:        809     963     466

  Fragments arranged by size: 

                 963     809     466
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1017    1127    1235    1945    2238 
   Size:       1017     110     108     710     293

  Fragments arranged by size: 

                1017     710     293     110     108
 
AhdI GACnn_n'nnGTC

Cuts at:      0     495    2238 
   Size:        495    1743
 
AleI CACnn'nnGTG

Cuts at:      0    1597    2238 
   Size:       1597     641
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1831    1863    2238 
   Size:       1831      32     375

  Fragments arranged by size: 

                1831     375      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1867    2238 
   Size:       1867     371
 
ApaLI G'TGCA_C

Cuts at:      0     232     511    2238 
   Size:        232     279    1727

  Fragments arranged by size: 

                1727     279     232
 
ApoI r'AATT_y

Cuts at:      0    1435    2238 
   Size:       1435     803
 
AseI AT'TA_AT

Cuts at:      0    1115    2238 
   Size:       1115    1123
 
AvaI C'yCGr_G

Cuts at:      0    2060    2238 
   Size:       2060     178
 
BanI G'GyrC_C

Cuts at:      0     809    1772    2238 
   Size:        809     963     466

  Fragments arranged by size: 

                 963     809     466
 
BanII G_rGCy'C

Cuts at:      0     421    2238 
   Size:        421    1817
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2090    2124    2238 
   Size:       2090      34     114

  Fragments arranged by size: 

                2090     114      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     468    1813    2238 
   Size:        468    1345     425

  Fragments arranged by size: 

                1345     468     425
 
BlpI GC'TnA_GC

Cuts at:      0    1078    1684    2238 
   Size:       1078     606     554

  Fragments arranged by size: 

                1078     606     554
 
Bme1580I G_kGCm'C

Cuts at:      0     236     515    1533    2238 
   Size:        236     279    1018     705

  Fragments arranged by size: 

                1018     705     279     236
 
BmgBI CAC'GTC

Cuts at:      0     618     855    2238 
   Size:        618     237    1383

  Fragments arranged by size: 

                1383     618     237
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     140    1515    2238 
   Size:        140    1375     723

  Fragments arranged by size: 

                1375     723     140
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     405    1063    1367    2238 
   Size:        252     153     658     304     871

  Fragments arranged by size: 

                 871     658     304     252     153
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     482    1476    2202    2238 
   Size:        482     994     726      36

  Fragments arranged by size: 

                 994     726     482      36
 
BsaAI yAC'GTr

Cuts at:      0     351    1318    2238 
   Size:        351     967     920

  Fragments arranged by size: 

                 967     920     351
 
BsaBI GATnn'nnATC

Cuts at:      0    1824    2238 
   Size:       1824     414
 
BsaHI Gr'CG_yC

Cuts at:      0    1676    2238 
   Size:       1676     562
 
BsaWI w'CCGG_w

Cuts at:      0     150    2238 
   Size:        150    2088
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     730     753     760     783    1831    1861    1885    1915 
   Size:        730      23       7      23    1048      30      24      30

Cuts at:   1915    2238 
   Size:        323

  Fragments arranged by size: 

                1048     730     323      30      30      24      23      23
                   7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     108     761     871    2238 
   Size:        108     653     110    1367

  Fragments arranged by size: 

                1367     653     110     108
 
BseYI C'CCAG_C

Cuts at:      0     442    1481    2096    2238 
   Size:        442    1039     615     142

  Fragments arranged by size: 

                1039     615     442     142
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     553     967    2238 
   Size:        553     414    1271

  Fragments arranged by size: 

                1271     553     414
 
BsiHKAI G_wGCw'C

Cuts at:      0     236     515     881    1685    2238 
   Size:        236     279     366     804     553

  Fragments arranged by size: 

                 804     553     366     279     236
 
BsmI GAATG_Cn'

Cuts at:      0     846    2238 
   Size:        846    1392
 
Bsp1286I G_dGCh'C

Cuts at:      0     236     421     515     881    1533    1685    2238 
   Size:        236     185      94     366     652     152     553

  Fragments arranged by size: 

                 652     553     366     236     185     152      94
 
BspEI T'CCGG_A

Cuts at:      0     150    2238 
   Size:        150    2088
 
BspHI T'CATG_A

Cuts at:      0    1967    2238 
   Size:       1967     271
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     898    1919    2238 
   Size:        898    1021     319

  Fragments arranged by size: 

                1021     898     319
 
BsrBI CCG'CTC

Cuts at:      0    1812    2238 
   Size:       1812     426
 
BsrDI GCAATG_nn'

Cuts at:      0    1089    2238 
   Size:       1089    1149
 
BssHII G'CGCG_C

Cuts at:      0    1780    2238 
   Size:       1780     458
 
BssSI C'ACGA_G

Cuts at:      0    1010    1501    2238 
   Size:       1010     491     737

  Fragments arranged by size: 

                1010     737     491
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     290    2238 
   Size:        290    1948
 
BstEII G'GTnAC_C

Cuts at:      0    1458    2238 
   Size:       1458     780
 
BstYI r'GATC_y

Cuts at:      0     523    2238 
   Size:        523    1715
 
BstZ17I GTA'TAC

Cuts at:      0     354    2238 
   Size:        354    1884
 
BtgI C'CryG_G

Cuts at:      0    1850    2238 
   Size:       1850     388
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     655    1157    2238 
   Size:        655     502    1081

  Fragments arranged by size: 

                1081     655     502
 
BtsI GCAGTG_nn'

Cuts at:      0    1439    1592    2238 
   Size:       1439     153     646

  Fragments arranged by size: 

                1439     646     153
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1213    1248    1703    1738    2238 
   Size:       1213      35     455      35     500

  Fragments arranged by size: 

                1213     500     455      35      35
 
DraI TTT'AAA

Cuts at:      0    1421    2187    2238 
   Size:       1421     766      51

  Fragments arranged by size: 

                1421     766      51
 
DraIII CAC_nnn'GTG

Cuts at:      0    1742    2238 
   Size:       1742     496
 
EaeI y'GGCC_r

Cuts at:      0     407     633     647    1946    2238 
   Size:        407     226      14    1299     292

  Fragments arranged by size: 

                1299     407     292     226      14
 
EarI CTCTTCn'nnn_

Cuts at:      0     799    2238 
   Size:        799    1439
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     405    1017    1063    1127    1235    1367    1945 
   Size:        252     153     612      46      64     108     132     578

Cuts at:   1945    2238 
   Size:        293

  Fragments arranged by size: 

                 612     578     293     252     153     132     108      64
                  46
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1693    2238 
   Size:       1693     545
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1028    2238 
   Size:       1028    1210
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     250     282     481     513    2142    2174    2182    2214 
   Size:        250      32     199      32    1629      32       8      32

Cuts at:   2214    2238 
   Size:         24

  Fragments arranged by size: 

                1629     250     199      32      32      32      32      24
                   8
 
HincII GTy'rAC

Cuts at:      0    1859    2238 
   Size:       1859     379
 
HindIII A'AGCT_T

Cuts at:      0    1495    2238 
   Size:       1495     743
 
KpnI G_GTAC'C

Cuts at:      0     813    1776    2238 
   Size:        813     963     462

  Fragments arranged by size: 

                 963     813     462
 
MfeI C'AATT_G

Cuts at:      0     208    1942    1950    2238 
   Size:        208    1734       8     288

  Fragments arranged by size: 

                1734     288     208       8
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     702    2238 
   Size:        702    1536
 
MscI TGG'CCA

Cuts at:      0     649    1948    2238 
   Size:        649    1299     290

  Fragments arranged by size: 

                1299     649     290
 
MslI CAynn'nnrTG

Cuts at:      0    1597    1830    1966    2238 
   Size:       1597     233     136     272

  Fragments arranged by size: 

                1597     272     233     136
 
MspA1I CmG'CkG

Cuts at:      0      37     122    1583    2129    2148    2238 
   Size:         37      85    1461     546      19      90

  Fragments arranged by size: 

                1461     546      90      85      37      19
 
NcoI C'CATG_G

Cuts at:      0    1850    2238 
   Size:       1850     388
 
NspI r_CATG'y

Cuts at:      0     789    2145    2238 
   Size:        789    1356      93

  Fragments arranged by size: 

                1356     789      93
 
PfoI T'CCnGG_A

Cuts at:      0     173    1040    1344    2238 
   Size:        173     867     304     894

  Fragments arranged by size: 

                 894     867     304     173
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1831    1863    2238 
   Size:       1831      32     375

  Fragments arranged by size: 

                1831     375      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1028    2238 
   Size:       1028    1210
 
PstI C_TGCA'G

Cuts at:      0    1446    1512    2238 
   Size:       1446      66     726

  Fragments arranged by size: 

                1446     726      66
 
PvuII CAG'CTG

Cuts at:      0      37    2129    2238 
   Size:         37    2092     109

  Fragments arranged by size: 

                2092     109      37
 
ScaI AGT'ACT

Cuts at:      0     666    1525    2238 
   Size:        666     859     713

  Fragments arranged by size: 

                 859     713     666
 
SexAI A'CCwGG_T

Cuts at:      0    2192    2238 
   Size:       2192      46
 
SfcI C'TryA_G

Cuts at:      0    1442    1508    2238 
   Size:       1442      66     730

  Fragments arranged by size: 

                1442     730      66
 
SnaBI TAC'GTA

Cuts at:      0     351    2238 
   Size:        351    1887
 
SspI AAT'ATT

Cuts at:      0    1301    1885    2238 
   Size:       1301     584     353

  Fragments arranged by size: 

                1301     584     353
 
StyI C'CwwG_G

Cuts at:      0     199    1850    2238 
   Size:        199    1651     388

  Fragments arranged by size: 

                1651     388     199
 
TatI w'GTAC_w

Cuts at:      0     664     782    1523    2238 
   Size:        664     118     741     715

  Fragments arranged by size: 

                 741     715     664     118
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     753     785    2238 
   Size:        753      32    1453

  Fragments arranged by size: 

                1453     753      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2176    2194    2238 
   Size:       2176      18      44

  Fragments arranged by size: 

                2176      44      18
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     999    2238 
   Size:        999    1239
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AhdI     AleI     AloI    AlwNI
    ApaLI     ApoI     AseI     AvaI     BanI    BanII     BcgI    BciVI
     BlpI Bme1580I    BmgBI     BmrI     BpmI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI
   BstAPI   BstEII    BstYI  BstZ17I     BtgI    BtgZI     BtsI    CspCI
     DraI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I    Hin4I
   HincII  HindIII     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
     NcoI     NspI     PfoI     PpiI    PpuMI     PstI    PvuII     ScaI
    SexAI     SfcI    SnaBI     SspI     StyI     TatI     TstI  Tth111I
     XcmI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AlfI     ApaI
     AscI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BclI    BfrBI     BglI    BglII     BmtI     BplI   Bpu10I    BpuEI
    BsiEI    BsiWI    BsmBI    BsrFI    BsrGI    BstBI    BstXI   Bsu36I
     ClaI     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     XbaI     XhoI
     XmaI     XmnI     ZraI
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