Restriction map for pF1KE0786
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# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        AocI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbsI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BpuAI       BsaBI       BsaI        
BsiWI       BsoBI       Bsp1407I    BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CpoI        DraI        DraIII      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        MaeI        MamI        MfeI        MluNI       
MscI        MseI        MunI        NdeI        NheI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccIII       1121  TCCGGA               T^CCGGA
AflIII        896  ACGCGT               A^C[GA][CT]GT
AhdI          410  GACCGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1084  GTGCAC               G^TGCAC
AlwI          207  GATCC                GGATC(4/5)
AlwNI         436  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       695  AGCGCT               AGC^GCT
ApaI          248  GGGCCC               GGGCC^C
ApaLI        1084  GTGCAC               G^TGCAC
AspEI         410  GACCGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        1081  CACGTG               CAC^GTG
BglI          124  GCCGGAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          150  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      150  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1081  CACGTG               [CT]AC^GT[GA]
BsaWI        1121  TCCGGA               [AT]^CCGG[AT]
BseAI        1121  TCCGGA               T^CCGGA
BsgI          784  GTGCAG               GTGCAG(16/14)
BsmBI         327  CGTCTC               CGTCTC(1/5)
BsmI          168  GAATGC               GAATGC(1/-1)
BspDI         157  ATCGAT               AT^CGAT
BspEI        1121  TCCGGA               T^CCGGA
BspMI        1270  ACCTGC               ACCTGC(4/8)
BsrBI         403  CCGCTC               CCGCTC(-3/-3)
BstAPI       1190  GCACGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI          31  CCAGCACCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         206  AGATCC               [GA]^GATC[CT]
CelII         150  GCTGAGC              GC^T[ACGT]AGC
ClaI          157  ATCGAT               AT^CGAT
DrdI          480  GACAGGCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      410  GACCGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          946  GAAGAG               CTCTTC(1/4)
Eco47III      695  AGCGCT               AGC^GCT
EcoNI         942  CCTGGAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Ksp632I       946  GAAGAG               CTCTTC(1/4)
MflI          206  AGATCC               [GA]^GATC[CT]
MluI          896  ACGCGT               A^CGCGT
MroI         1121  TCCGGA               T^CCGGA
MslI         1201  CATGGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          744  GCCGGC               GCC^GGC
NcoI         1200  CCATGG               C^CATGG
NgoMIV        744  GCCGGC               G^CCGGC
NotI          465  GCGGCCGC             GC^GGCCGC
PleI          834  GACTC                GAGTC(4/5)
PmaCI        1081  CACGTG               CAC^GTG
PmlI         1081  CACGTG               CAC^GTG
PpuMI         938  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          763  CTGCAG               CTGCA^G
SexAI         878  ACCTGGT              A^CC[AT]GGT
SfcI          763  CTGCAG               C^T[GA][CT]AG
SfiI          123  GGCCGGAGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI            4  GATTC                G^A[AT]TC
XhoII         206  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AfaI          309  GTAC                 GT^AC
              900  GTAC                
BanII         248  GGGCCC               G[GA]GC[CT]^C
              622  GAGCCC              
BbeI          360  GGCGCC               GGCGC^C
              973  GGCGCC              
BbvCI         228  CCTCAGC              CCTCAGC(-5/-2)
              240  CCTCAGC             
BcgI          693  CGAGCGCTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              716  CGAGCACAGTGC        
BpmI           22  CTGGAG               CTGGAG(16/14)
             1240  CTGGAG              
BsmAI        1169  GAGAC                GTCTC(1/5)
              328  GTCTC               
BsrFI         255  GCCGGT               [GA]^CCGG[CT]
              744  GCCGGC              
Cfr10I        255  GCCGGT               [GA]^CCGG[CT]
              744  GCCGGC              
EagI          466  CGGCCG               C^GGCCG
              472  CGGCCG              
EclXI         466  CGGCCG               C^GGCCG
              472  CGGCCG              
Eco52I        466  CGGCCG               C^GGCCG
              472  CGGCCG              
EcoT14I        37  CCTTGG               C^C[AT][AT]GG
             1200  CCATGG              
HinfI           4  GATTC                G^A[ACGT]TC
              834  GACTC               
HphI          139  GGTGA                GGTGA(8/7)
              846  TCACC               
KasI          360  GGCGCC               G^GCGCC
              973  GGCGCC              
MaeII        1001  ACGT                 A^CGT
             1082  ACGT                
NarI          360  GGCGCC               GG^CGCC
              973  GGCGCC              
RsaI          309  GTAC                 GT^AC
              900  GTAC                
SfoI          360  GGCGCC               GGC^GCC
              973  GGCGCC              
StuI          278  AGGCCT               AGG^CCT
             1336  AGGCCT              
StyI           37  CCTTGG               C^C[AT][AT]GG
             1200  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AspHI       
AvaII       BanI        BbvI        BcnI        BmyI        
BsaHI       BsaJI       BseRI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
ItaI        KspI        MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PvuII       SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TseI        Tsp45I      TspRI       
TthHB8I     
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