Restriction map for pF1KE0786
 (Linear) (Six-base) MAPSORT of: KIEE0786.seq  Check: 7047  from: 1  to: 1371



KIEE0786.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1279    1371 
   Size:       1279      92
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580    1087    1239    1371 
   Size:        580     507     152     132

  Fragments arranged by size: 

                 580     507     152     132
 
AfeI AGC'GCT

Cuts at:      0     697    1371 
   Size:        697     674
 
AflIII A'CryG_T

Cuts at:      0     896    1371 
   Size:        896     475
 
AhdI GACnn_n'nnGTC

Cuts at:      0     415    1371 
   Size:        415     956
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     809    1371 
   Size:        809     562
 
AlwNI CAG_nnn'CTG

Cuts at:      0     441    1371 
   Size:        441     930
 
ApaI G_GGCC'C

Cuts at:      0     252    1371 
   Size:        252    1119
 
ApaLI G'TGCA_C

Cuts at:      0    1084    1371 
   Size:       1084     287
 
BanI G'GyrC_C

Cuts at:      0     360     523     576     804     973    1371 
   Size:        360     163      53     228     169     398

  Fragments arranged by size: 

                 398     360     228     169     163      53
 
BanII G_rGCy'C

Cuts at:      0     252     626    1371 
   Size:        252     374     745

  Fragments arranged by size: 

                 745     374     252
 
BbeI G_GCGC'C

Cuts at:      0     364     977    1371 
   Size:        364     613     394

  Fragments arranged by size: 

                 613     394     364
 
BbvCI CC'TCA_GC

Cuts at:      0     229     241    1371 
   Size:        229      12    1130

  Fragments arranged by size: 

                1130     229      12
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     682     705     716     739    1371 
   Size:        682      23      11      23     632

  Fragments arranged by size: 

                 682     632      23      23      11
 
BglI GCCn_nnn'nGGC

Cuts at:      0     130    1371 
   Size:        130    1241
 
BlpI GC'TnA_GC

Cuts at:      0     151    1371 
   Size:        151    1220
 
Bme1580I G_kGCm'C

Cuts at:      0      80     252     581    1088    1157    1371 
   Size:         80     172     329     507      69     214

  Fragments arranged by size: 

                 507     329     214     172      80      69
 
BmgBI CAC'GTC

Cuts at:      0    1002    1371 
   Size:       1002     369
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     153    1371 
   Size:        153    1218
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43    1261    1371 
   Size:         43    1218     110

  Fragments arranged by size: 

                1218     110      43
 
Bpu10I CC'TnA_GC

Cuts at:      0     229     241    1371 
   Size:        229      12    1130

  Fragments arranged by size: 

                1130     229      12
 
BsaAI yAC'GTr

Cuts at:      0    1083    1371 
   Size:       1083     288
 
BsaHI Gr'CG_yC

Cuts at:      0     361     974    1114    1371 
   Size:        361     613     140     257

  Fragments arranged by size: 

                 613     361     257     140
 
BsaWI w'CCGG_w

Cuts at:      0    1121    1371 
   Size:       1121     250
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     119     149     389     419     904     934    1371 
   Size:        119      30     240      30     485      30     437

  Fragments arranged by size: 

                 485     437     240     119      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     565     925     979    1159    1371 
   Size:        565     360      54     180     212

  Fragments arranged by size: 

                 565     360     212     180      54
 
BseYI C'CCAG_C

Cuts at:      0     251     322     594     600     646     888    1015    1225 
   Size:        251      71     272       6      46     242     127     210

Cuts at:   1225    1371 
   Size:        146

  Fragments arranged by size: 

                 272     251     242     210     146     127      71      46
                   6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     805    1371 
   Size:        805     566
 
BsiEI CG_ry'CG

Cuts at:      0     412     469     475    1371 
   Size:        412      57       6     896

  Fragments arranged by size: 

                 896     412      57       6
 
BsiHKAI G_wGCw'C

Cuts at:      0     557     721    1088    1371 
   Size:        557     164     367     283

  Fragments arranged by size: 

                 557     367     283     164
 
BsmI GAATG_Cn'

Cuts at:      0     174    1371 
   Size:        174    1197
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     333    1371 
   Size:        333    1038
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     252     557     581     626     721    1088    1157 
   Size:         80     172     305      24      45      95     367      69

Cuts at:   1157    1371 
   Size:        214

  Fragments arranged by size: 

                 367     305     214     172      95      80      69      45
                  24
 
BspEI T'CCGG_A

Cuts at:      0    1121    1371 
   Size:       1121     250
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1279    1371 
   Size:       1279      92
 
BsrBI CCG'CTC

Cuts at:      0     405    1371 
   Size:        405     966
 
BsrFI r'CCGG_y

Cuts at:      0     255     744    1371 
   Size:        255     489     627

  Fragments arranged by size: 

                 627     489     255
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1196    1371 
   Size:       1196     175
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      38    1371 
   Size:         38    1333
 
BstYI r'GATC_y

Cuts at:      0     206    1371 
   Size:        206    1165
 
BtgI C'CryG_G

Cuts at:      0     134     293     304     469    1200    1371 
   Size:        134     159      11     165     731     171

  Fragments arranged by size: 

                 731     171     165     159     134      11
 
BtsI GCAGTG_nn'

Cuts at:      0    1086    1371 
   Size:       1086     285
 
ClaI AT'CG_AT

Cuts at:      0     158    1371 
   Size:        158    1213
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     486    1371 
   Size:        486     885
 
EaeI y'GGCC_r

Cuts at:      0     131     466     472     746    1371 
   Size:        131     335       6     274     625

  Fragments arranged by size: 

                 625     335     274     131       6
 
EagI C'GGCC_G

Cuts at:      0     466     472    1371 
   Size:        466       6     899

  Fragments arranged by size: 

                 899     466       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     941    1371 
   Size:        941     430
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     273    1371 
   Size:        273    1098
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     580    1087    1239    1261    1371 
   Size:         43     537     507     152      22     110

  Fragments arranged by size: 

                 537     507     152     110      43      22
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     946    1371 
   Size:        946     425
 
EcoO109I rG'GnC_Cy

Cuts at:      0     248     939    1130    1245    1371 
   Size:        248     691     191     115     126

  Fragments arranged by size: 

                 691     248     191     126     115
 
HaeII r_GCGC'y

Cuts at:      0     228     364     383     699     889     977    1371 
   Size:        228     136      19     316     190      88     394

  Fragments arranged by size: 

                 394     316     228     190     136      88      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     389     401     421     433    1371 
   Size:        389      12      20      12     938

  Fragments arranged by size: 

                 938     389      20      12      12
 
KasI G'GCGC_C

Cuts at:      0     360     973    1371 
   Size:        360     613     398

  Fragments arranged by size: 

                 613     398     360
 
MluI A'CGCG_T

Cuts at:      0     896    1371 
   Size:        896     475
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     432    1371 
   Size:        432     939
 
MslI CAynn'nnrTG

Cuts at:      0    1205    1371 
   Size:       1205     166
 
MspA1I CmG'CkG

Cuts at:      0     295     306     373     414     438     471     762     793 
   Size:        295      11      67      41      24      33     291      31

Cuts at:    793    1019    1259    1371 
   Size:        226     240     112

  Fragments arranged by size: 

                 295     291     240     226     112      67      41      33
                  31      24      11
 
NaeI GCC'GGC

Cuts at:      0     746    1371 
   Size:        746     625
 
NarI GG'CG_CC

Cuts at:      0     361     974    1371 
   Size:        361     613     397

  Fragments arranged by size: 

                 613     397     361
 
NcoI C'CATG_G

Cuts at:      0    1200    1371 
   Size:       1200     171
 
NgoMIV G'CCGG_C

Cuts at:      0     744    1371 
   Size:        744     627
 
NotI GC'GGCC_GC

Cuts at:      0     466    1371 
   Size:        466     905
 
PasI CC'CwG_GG

Cuts at:      0     668    1248    1371 
   Size:        668     580     123

  Fragments arranged by size: 

                 668     580     123
 
PfoI T'CCnGG_A

Cuts at:      0     941    1238    1371 
   Size:        941     297     133

  Fragments arranged by size: 

                 941     297     133
 
PmlI CAC'GTG

Cuts at:      0    1083    1371 
   Size:       1083     288
 
PpuMI rG'GwC_Cy

Cuts at:      0     939    1371 
   Size:        939     432
 
PspOMI G'GGCC_C

Cuts at:      0     248    1371 
   Size:        248    1123
 
PstI C_TGCA'G

Cuts at:      0     767    1371 
   Size:        767     604
 
PvuII CAG'CTG

Cuts at:      0     793    1019    1259    1371 
   Size:        793     226     240     112

  Fragments arranged by size: 

                 793     240     226     112
 
SacII CC_GC'GG

Cuts at:      0     296     307     472    1371 
   Size:        296      11     165     899

  Fragments arranged by size: 

                 899     296     165      11
 
SexAI A'CCwGG_T

Cuts at:      0     878    1371 
   Size:        878     493
 
SfcI C'TryA_G

Cuts at:      0     763    1371 
   Size:        763     608
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     130    1371 
   Size:        130    1241
 
SfoI GGC'GCC

Cuts at:      0     362     975    1371 
   Size:        362     613     396

  Fragments arranged by size: 

                 613     396     362
 
StuI AGG'CCT

Cuts at:      0     280    1338    1371 
   Size:        280    1058      33

  Fragments arranged by size: 

                1058     280      33
 
StyI C'CwwG_G

Cuts at:      0      37    1200    1371 
   Size:         37    1163     171

  Fragments arranged by size: 

                1163     171      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     127     135     336    1029    1371 
   Size:        127       8     201     693     342

  Fragments arranged by size: 

                 693     342     201     127       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     119     151     904     936    1371 
   Size:        119      32     753      32     435

  Fragments arranged by size: 

                 753     435     119      32      32
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AhdI     AlfI    AlwNI     ApaI
    ApaLI     BanI    BanII     BbeI    BbvCI     BcgI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BstAPI    BstXI    BstYI
     BtgI     BtsI     ClaI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KasI     MluI     MmeI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     PasI
     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII    SacII    SexAI
     SfcI     SfiI     SfoI     StuI     StyI    TaqII     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AleI     AloI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbsI    BciVI     BclI    BfrBI    BglII     BmtI     BplI    BpuEI
     BsaI    BsaBI    BsiWI    BspHI    BsrDI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI    CspCI     DraI   DraIII
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MscI     NdeI     NheI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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