Restriction map for pF1KB5437
 (Circular) (Six-base) MAPSORT of: pF1KB5437.seq  Check: 4939  from: 1  to: 4112



pF1KB5437  4112 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:   1150    1150 
   Size:       4112
 
Acc65I G'GTAC_C

Cuts at:   1128    1128 
   Size:       4112
 
AclI AA'CG_TT

Cuts at:   3952    3952 
   Size:       4112
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1937    2369    3116    3384    1937 
   Size:        432     747     268    2665

  Fragments arranged by size: 

                2665     747     432     268
 
AflIII A'CryG_T

Cuts at:   1464    2568    3545    1464 
   Size:       1104     977    2031

  Fragments arranged by size: 

                2031    1104     977
 
AgeI A'CCGG_T

Cuts at:   2500    2500 
   Size:       4112
 
AlwNI CAG_nnn'CTG

Cuts at:    130     458     499     763    1417    2984     130 
   Size:        328      41     264     654    1567    1258

  Fragments arranged by size: 

                1567    1258     654     328     264      41
 
ApaLI G'TGCA_C

Cuts at:   2882    2882 
   Size:       4112
 
ApoI r'AATT_y

Cuts at:    610    1116    3465    3721     610 
   Size:        506    2349     256    1001

  Fragments arranged by size: 

                2349    1001     506     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4112
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4112
 
AvaI C'yCGr_G

Cuts at:   1132    1132 
   Size:       4112
 
BamHI G'GATC_C

Cuts at:   1137    1137 
   Size:       4112
 
BanI G'GyrC_C

Cuts at:   1128    1542    1790    1825    1128 
   Size:        414     248      35    3415

  Fragments arranged by size: 

                3415     414     248      35
 
BanII G_rGCy'C

Cuts at:   1126    2156    1126 
   Size:       1030    3082
 
BbeI G_GCGC'C

Cuts at:   1546    1794    1546 
   Size:        248    3864
 
BciVI GTATCCnnnnn_n'

Cuts at:   2010    2771    4112    2010 
   Size:        761    1341    2010

  Fragments arranged by size: 

                2010    1341     761
 
BclI T'GATC_A

Cuts at:    575    1632     575 
   Size:       1057    3055
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       4112
 
BglII A'GATC_T

Cuts at:    956    1627    3664     956 
   Size:        671    2037    1404

  Fragments arranged by size: 

                2037    1404     671
 
BlpI GC'TnA_GC

Cuts at:   1224    1224 
   Size:       4112
 
Bme1580I G_kGCm'C

Cuts at:   1737    1830    2886    1737 
   Size:         93    1056    2963

  Fragments arranged by size: 

                2963    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1324    1405    1740    3884    1324 
   Size:         81     335    2144    1552

  Fragments arranged by size: 

                2144    1552     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2479    2479 
   Size:       4112
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    674     674 
   Size:       4112
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    539    1288    2167    2659    2957     539 
   Size:        749     879     492     298    1694

  Fragments arranged by size: 

                1694     879     749     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3816    3816 
   Size:       4112
 
BsaAI yAC'GTr

Cuts at:   1465    1465 
   Size:       4112
 
BsaBI GATnn'nnATC

Cuts at:      6    1626    3663       6 
   Size:       1620    2037     455

  Fragments arranged by size: 

                2037    1620     455
 
BsaHI Gr'CG_yC

Cuts at:   1543    1791    2493    3989    1543 
   Size:        248     702    1496    1666

  Fragments arranged by size: 

                1666    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1301    1822    2500    2774    2921    3332    1301 
   Size:        521     678     274     147     411    2081

  Fragments arranged by size: 

                2081     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    217     217 
   Size:       4112
 
BseYI C'CCAG_C

Cuts at:   1537    2295    2872    3368    1537 
   Size:        758     577     496    2281

  Fragments arranged by size: 

                2281     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    458     458 
   Size:       4112
 
BsiEI CG_ry'CG

Cuts at:    104    1654    1700    2908     104 
   Size:       1550      46    1208    1308

  Fragments arranged by size: 

                1550    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    712    1126    1904    2094    2886     712 
   Size:        414     778     190     792    1938

  Fragments arranged by size: 

                1938     792     778     414     190
 
Bsp1286I G_dGCh'C

Cuts at:    712    1126    1737    1830    1904    2094    2156    2886     712 
   Size:        414     611      93      74     190      62     730    1938

  Fragments arranged by size: 

                1938     730     611     414     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3332    3332 
   Size:       4112
 
BspHI T'CATG_A

Cuts at:   3288    3288 
   Size:       4112
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1162    1376    1678    2059    1162 
   Size:        214     302     381    3215

  Fragments arranged by size: 

                3215     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2404    2458    3942    4107      50 
   Size:       2354      54    1484     165      55

  Fragments arranged by size: 

                2354    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2024    2024 
   Size:       4112
 
BsrFI r'CCGG_y

Cuts at:   2110    2291    2500    2110 
   Size:        181     209    3722

  Fragments arranged by size: 

                3722     209     181
 
BssSI C'ACGA_G

Cuts at:   2383    2741    2383 
   Size:        358    3754
 
BstAPI GCAn_nnn'nTGC

Cuts at:    130    1489     130 
   Size:       1359    2753
 
BstBI TT'CG_AA

Cuts at:   2473    2473 
   Size:       4112
 
BstYI r'GATC_y

Cuts at:      1     956    1137    1604    1627    1962    2208    3220    3306 
   Size:        955     181     467      23     335     246    1012      86

Cuts at:   3306    3318    3664       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     955     467     449     346     335     246     181
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4112
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2062    2243    2406    2062 
   Size:        181     163    3768

  Fragments arranged by size: 

                3768     181     163
 
BtsI GCAGTG_nn'

Cuts at:    344    1355     344 
   Size:       1011    3101
 
DraI TTT'AAA

Cuts at:    717     938    1111     717 
   Size:        221     173    3718

  Fragments arranged by size: 

                3718     221     173
 
DrdI GACnn_nn'nnGTC

Cuts at:    516    1818    2676     516 
   Size:       1302     858    1952

  Fragments arranged by size: 

                1952    1302     858
 
EaeI y'GGCC_r

Cuts at:   1697    1871    2262    2289    3634    1697 
   Size:        174     391      27    1345    2175

  Fragments arranged by size: 

                2175    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1697    1697 
   Size:       4112
 
EarI CTCTTCn'nnn_

Cuts at:   2135    2345    2135 
   Size:        210    3902
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2320    2630    2776    3922    2320 
   Size:        310     146    1146    2510

  Fragments arranged by size: 

                2510    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1124    1124 
   Size:       4112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    674    1937    2369    3116    3384     674 
   Size:       1263     432     747     268    1402

  Fragments arranged by size: 

                1402    1263     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    183    1251     183 
   Size:       1068    3044
 
EcoRI G'AATT_C

Cuts at:   1116    1116 
   Size:       4112
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2131    2131 
   Size:       4112
 
FspI TGC'GCA

Cuts at:   1893    1893 
   Size:       4112
 
HaeII r_GCGC'y

Cuts at:   1546    1794    2816    3795    1546 
   Size:        248    1022     979    1863

  Fragments arranged by size: 

                1863    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1954    1984    1986    2016    1954 
   Size:         30       2      30    4050

  Fragments arranged by size: 

                4050      30      30       2
 
HincII GTy'rAC

Cuts at:   1151    1151 
   Size:       4112
 
KasI G'GCGC_C

Cuts at:   1542    1790    1542 
   Size:        248    3864
 
KpnI G_GTAC'C

Cuts at:   1132    1132 
   Size:       4112
 
MluI A'CGCG_T

Cuts at:   3545    3545 
   Size:       4112
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    899    2783    2967     899 
   Size:       1884     184    2044

  Fragments arranged by size: 

                2044    1884     184
 
MscI TGG'CCA

Cuts at:   1873    1873 
   Size:       4112
 
MslI CAynn'nnrTG

Cuts at:    349    2101    2228     349 
   Size:       1752     127    2233

  Fragments arranged by size: 

                2233    1752     127
 
MspA1I CmG'CkG

Cuts at:   1223    1537    1897    2910    3155    1223 
   Size:        314     360    1013     245    2180

  Fragments arranged by size: 

                2180    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2293    2293 
   Size:       4112
 
NarI GG'CG_CC

Cuts at:   1543    1791    1543 
   Size:        248    3864
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4112
 
NgoMIV G'CCGG_C

Cuts at:   2291    2291 
   Size:       4112
 
NspI r_CATG'y

Cuts at:   1165    1665    2572    1165 
   Size:        500     907    2705

  Fragments arranged by size: 

                2705     907     500
 
PciI A'CATG_T

Cuts at:   2568    2568 
   Size:       4112
 
PmeI GTTT'AAAC

Cuts at:   1111    1111 
   Size:       4112
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3251    3283    3251 
   Size:         32    4080
 
PpuMI rG'GwC_Cy

Cuts at:    183     183 
   Size:       4112
 
PstI C_TGCA'G

Cuts at:    123    1159    1844     123 
   Size:       1036     685    2391

  Fragments arranged by size: 

                2391    1036     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4112
 
PvuII CAG'CTG

Cuts at:   1537    1897    1537 
   Size:        360    3752
 
RsrII CG'GwC_CG

Cuts at:   2307    2307 
   Size:       4112
 
SacI G_AGCT'C

Cuts at:   1126    1126 
   Size:       4112
 
SalI G'TCGA_C

Cuts at:   1149    1149 
   Size:       4112
 
SapI GCTCTTCn'nnn_

Cuts at:   2135    2345    2135 
   Size:        210    3902
 
SbfI CC_TGCA'GG

Cuts at:   1159    1159 
   Size:       4112
 
ScaI AGT'ACT

Cuts at:    722    3629     722 
   Size:       2907    1205
 
SfcI C'TryA_G

Cuts at:     33     119    1155    1840    2833    3024      33 
   Size:         86    1036     685     993     191    1121

  Fragments arranged by size: 

                1121    1036     993     685     191      86
 
SfoI GGC'GCC

Cuts at:   1544    1792    1544 
   Size:        248    3864
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4112
 
SmaI CCC'GGG

Cuts at:   1134    1134 
   Size:       4112
 
SmlI C'TyrA_G

Cuts at:    554    1267    2182    2674    2936     554 
   Size:        713     915     492     262    1730

  Fragments arranged by size: 

                1730     915     713     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4112
 
SphI G_CATG'C

Cuts at:   1165    1665    1165 
   Size:        500    3612
 
StyI C'CwwG_G

Cuts at:    107    1246    1600     107 
   Size:       1139     354    2619

  Fragments arranged by size: 

                2619    1139     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1695    1828    2287    2496    1695 
   Size:        133     459     209    3311

  Fragments arranged by size: 

                3311     459     209     133
 
TatI w'GTAC_w

Cuts at:    720    3627     720 
   Size:       2907    1205
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    276     308    3629    3661     276 
   Size:         32    3321      32     727

  Fragments arranged by size: 

                3321     727      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1909    1909 
   Size:       4112
 
XbaI T'CTAG_A

Cuts at:    321    1143     321 
   Size:        822    3290
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1607    1607 
   Size:       4112
 
XmaI C'CCGG_G

Cuts at:   1132    1132 
   Size:       4112
 
XmnI GAAnn'nnTTC

Cuts at:    683     683 
   Size:       4112
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PpiI
    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
    BmgBI     BmtI   Bpu10I    BsaXI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     ZraI
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