Restriction map for pF1KE0203
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspI        
AvaI        AviII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BglI        BglII       
BlpI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsiYI       BslI        BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
TfiI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AciI          484  GCGG                 CCGC(-3/-1)
AfaI          160  GTAC                 GT^AC
AflII         240  CTTAAG               C^TTAAG
AseI          213  ATTAAT               AT^TAAT
AsnI          213  ATTAAT               AT^TAAT
AvaII         837  GGACC                G^G[AT]CC
AvrII         672  CCTAGG               C^CTAGG
BbvI          651  GCTGC                GCAGC(8/12)
BfrI          240  CTTAAG               C^TTAAG
BlnI          672  CCTAGG               C^CTAGG
BseRI         748  GAGGAG               GAGGAG(10/8)
BsmAI          99  GAGAC                GTCTC(1/5)
BspHI         282  TCATGA               T^CATGA
BstYI         522  AGATCC               [GA]^GATC[CT]
Cac8I         642  GCAGGC               GC[ACGT]^[ACGT]GC
Cfr13I        837  GGACC                G^G[ACGT]CC
EcoRI         425  GAATTC               G^AATTC
Fnu4HI        651  GCTGC                GC^[ACGT]GC
HaeIII        627  GGCC                 GG^CC
HphI          109  GGTGA                GGTGA(8/7)
ItaI          651  GCTGC                GC^[ACGT]GC
MaeIII        505  GTCAC                ^GT[ACGT]AC
MflI          522  AGATCC               [GA]^GATC[CT]
MwoI          642  GCAGGCAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PshBI         213  ATTAAT               AT^TAAT
PstI          352  CTGCAG               CTGCA^G
RcaI          282  TCATGA               T^CATGA
RsaI          160  GTAC                 GT^AC
SapI          589  GAAGAGC              GCTCTTC(1/4)
Sau96I        837  GGACC                G^G[ACGT]CC
SfcI          352  CTGCAG               C^T[GA][CT]AG
SmlI          240  CTTAAG               C^T[CT][GA]AG
SspI          886  AATATT               AAT^ATT
TaqI          429  TCGA                 T^CGA
TseI          651  GCTGC                G^C[AT]GC
Tsp45I        505  GTCAC                ^GT[GC]AC
TthHB8I       429  TCGA                 T^CGA
XhoII         522  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI          265  AAATTC               [GA]^AATT[CT]
              425  GAATTC              
AlwI          523  GATCC                GGATC(4/5)
              738  GGATC               
ApoI          265  AAATTC               [GA]^AATT[CT]
              425  GAATTC              
Asp700I        61  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              769  GAAGACATTC          
AspHI         146  GAGCAC               G[AT]GC[AT]^C
              322  GAGCAC              
BbsI          292  GAAGAC               GAAGAC(2/6)
              769  GAAGAC              
BpmI           83  CTCCAG               CTGGAG(16/14)
               92  CTCCAG              
BpuAI         292  GAAGAC               GAAGAC(2/6)
              769  GAAGAC              
BsaBI         303  GATGCTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              733  GATGAGGATC          
BsiHKAI       146  GAGCAC               G[AT]GC[AT]^C
              322  GAGCAC              
BssKI         387  CCTGG                ^CC[ACGT]GG
              701  CCAGG               
BstF5I        878  CATCC                GGATG(2/0)
              201  GGATG               
BstNI         387  CCTGG                CC^[AT]GG
              701  CCAGG               
EcoRII        387  CCTGG                ^CC[AT]GG
              701  CCAGG               
EcoT14I       629  CCTTGG               C^C[AT][AT]GG
              672  CCTAGG              
FokI          878  CATCC                GGATG(9/13)
              201  GGATG               
HinfI         133  GAGTC                G^A[ACGT]TC
              751  GAGTC               
MamI          303  GATGCTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              733  GATGAGGATC          
MvaI          387  CCTGG                CC^[AT]GG
              701  CCAGG               
NspI           48  GCATGT               [GA]CATG^[CT]
              246  GCATGT              
PleI          133  GAGTC                GAGTC(4/5)
              751  GAGTC               
ScrFI         387  CCTGG                CC^[ACGT]GG
              701  CCAGG               
StyI          629  CCTTGG               C^C[AT][AT]GG
              672  CCTAGG              
TspRI         325  CACTG                CA[GC]TG[ACGT][ACGT]^
              830  CACTG               
XmnI           61  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              769  GAAGACATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AluI        BfaI        BmyI        BsaJI       Bsp1286I    
BsrI        DdeI        DpnII       DraI        EarI        
Ksp632I     MaeI        MboI        MboII       MnlI        
MseI        NdeII       NlaIII      Sau3AI      SfaNI       
Tru9I       Tsp509I     
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