Restriction map for pF1KB5454
 (Circular) (Six-base) MAPSORT of: pF1KB5454.seq  Check: 1427  from: 1  to: 4655



pF1KB5454  4655 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AatII G_ACGT'C

Cuts at:   1607    1607 
   Size:       4655
 
AccI GT'mk_AC

Cuts at:   1000    1693    1000 
   Size:        693    3962
 
Acc65I G'GTAC_C

Cuts at:   1671    1671 
   Size:       4655
 
AclI AA'CG_TT

Cuts at:   4495    4495 
   Size:       4655
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    295     472    2480    2912    3659    3927     295 
   Size:        177    2008     432     747     268    1023

  Fragments arranged by size: 

                2008    1023     747     432     268     177
 
AfeI AGC'GCT

Cuts at:    184     184 
   Size:       4655
 
AflIII A'CryG_T

Cuts at:   2007    3111    4088    2007 
   Size:       1104     977    2574

  Fragments arranged by size: 

                2574    1104     977
 
AgeI A'CCGG_T

Cuts at:   3043    3043 
   Size:       4655
 
AhdI GACnn_n'nnGTC

Cuts at:   1113    1113 
   Size:       4655
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    950    1477     950 
   Size:        527    4128
 
AlwNI CAG_nnn'CTG

Cuts at:   1170    1960    3527    1170 
   Size:        790    1567    2298

  Fragments arranged by size: 

                2298    1567     790
 
ApaLI G'TGCA_C

Cuts at:   3425    3425 
   Size:       4655
 
ApoI r'AATT_y

Cuts at:   1659    4008    4264    1659 
   Size:       2349     256    2050

  Fragments arranged by size: 

                2349    2050     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4655
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4655
 
AvaI C'yCGr_G

Cuts at:    195    1675     195 
   Size:       1480    3175
 
BamHI G'GATC_C

Cuts at:   1680    1680 
   Size:       4655
 
BanI G'GyrC_C

Cuts at:   1671    2085    2333    2368    1671 
   Size:        414     248      35    3958

  Fragments arranged by size: 

                3958     414     248      35
 
BanII G_rGCy'C

Cuts at:   1027    1264    1669    2699    1027 
   Size:        237     405    1030    2983

  Fragments arranged by size: 

                2983    1030     405     237
 
BbeI G_GCGC'C

Cuts at:   2089    2337    2089 
   Size:        248    4407
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    279     313     279 
   Size:         34    4621
 
BciVI GTATCCnnnnn_n'

Cuts at:   2553    3314    4655    2553 
   Size:        761    1341    2553

  Fragments arranged by size: 

                2553    1341     761
 
BclI T'GATC_A

Cuts at:   2175    2175 
   Size:       4655
 
BglI GCCn_nnn'nGGC

Cuts at:    670     670 
   Size:       4655
 
BglII A'GATC_T

Cuts at:   2170    4207    2170 
   Size:       2037    2618
 
BlpI GC'TnA_GC

Cuts at:    826    1767     826 
   Size:        941    3714
 
Bme1580I G_kGCm'C

Cuts at:   2280    2373    3429    2280 
   Size:         93    1056    3506

  Fragments arranged by size: 

                3506    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1867    1948    2283    4427    1867 
   Size:         81     335    2144    2095

  Fragments arranged by size: 

                2144    2095     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1041    1302    3022    1041 
   Size:        261    1720    2674

  Fragments arranged by size: 

                2674    1720     261
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1264    1450    1264 
   Size:        186    4469
 
Bpu10I CC'TnA_GC

Cuts at:   1263    1263 
   Size:       4655
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1831    2710    3202    3500    1831 
   Size:        879     492     298    2986

  Fragments arranged by size: 

                2986     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1290    4359    1290 
   Size:       3069    1586
 
BsaAI yAC'GTr

Cuts at:   2008    2008 
   Size:       4655
 
BsaBI GATnn'nnATC

Cuts at:      6    2169    4206       6 
   Size:       2163    2037     455

  Fragments arranged by size: 

                2163    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1604    2086    2334    3036    4532    1604 
   Size:        482     248     702    1496    1727

  Fragments arranged by size: 

                1727    1496     702     482     248
 
BsaWI w'CCGG_w

Cuts at:   1844    2365    3043    3317    3464    3875    1844 
   Size:        521     678     274     147     411    2624

  Fragments arranged by size: 

                2624     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1237    1267    1237 
   Size:         30    4625
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    421    1168     421 
   Size:        747    3908
 
BseYI C'CCAG_C

Cuts at:    153     673    2080    2838    3415    3911     153 
   Size:        520    1407     758     577     496     897

  Fragments arranged by size: 

                1407     897     758     577     520     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    508     928     508 
   Size:        420    4235
 
BsiEI CG_ry'CG

Cuts at:    104     967    2197    2243    3451     104 
   Size:        863    1230      46    1208    1308

  Fragments arranged by size: 

                1308    1230    1208     863      46
 
BsiHKAI G_wGCw'C

Cuts at:   1669    2447    2637    3429    1669 
   Size:        778     190     792    2895

  Fragments arranged by size: 

                2895     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    163    1595     163 
   Size:       1432    3223
 
Bsp1286I G_dGCh'C

Cuts at:   1027    1264    1669    2280    2373    2447    2637    2699    3429 
   Size:        237     405     611      93      74     190      62     730

Cuts at:   3429    1027 
   Size:       2253

  Fragments arranged by size: 

                2253     730     611     405     237     190      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3875    3875 
   Size:       4655
 
BspHI T'CATG_A

Cuts at:    791    3831     791 
   Size:       3040    1615
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    983    1012    1705    1919    2221    2602     983 
   Size:         29     693     214     302     381    3036

  Fragments arranged by size: 

                3036     693     381     302     214      29
 
BsrBI CCG'CTC

Cuts at:     50     468     489    2947    3001    4485    4650      50 
   Size:        418      21    2458      54    1484     165      55

  Fragments arranged by size: 

                2458    1484     418     165      55      54      21
 
BsrDI GCAATG_nn'

Cuts at:    932    2567     932 
   Size:       1635    3020
 
BsrFI r'CCGG_y

Cuts at:   1319    2653    2834    3043    1319 
   Size:       1334     181     209    2931

  Fragments arranged by size: 

                2931    1334     209     181
 
BsrGI T'GTAC_A

Cuts at:    592     592 
   Size:       4655
 
BssSI C'ACGA_G

Cuts at:   2926    3284    2926 
   Size:        358    4297
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2032    2032 
   Size:       4655
 
BstBI TT'CG_AA

Cuts at:   1326    3016    1326 
   Size:       1690    2965
 
BstEII G'GTnAC_C

Cuts at:   1638    1638 
   Size:       4655
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     114 
   Size:       4655
 
BstYI r'GATC_y

Cuts at:      1     117    1680    2147    2170    2505    2751    3763    3849 
   Size:        116    1563     467      23     335     246    1012      86

Cuts at:   3849    3861    4207       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1563    1012     467     449     346     335     246     116
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     665    1029    1044     107 
   Size:        558     364      15    3718

  Fragments arranged by size: 

                3718     558     364      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2605    2786    2949    2605 
   Size:        181     163    4311

  Fragments arranged by size: 

                4311     181     163
 
BtsI GCAGTG_nn'

Cuts at:    876    1898     876 
   Size:       1022    3633
 
DraI TTT'AAA

Cuts at:   1654    1654 
   Size:       4655
 
DraIII CAC_nnn'GTG

Cuts at:   1191    1191 
   Size:       4655
 
DrdI GACnn_nn'nnGTC

Cuts at:   2361    3219    2361 
   Size:        858    3797
 
EaeI y'GGCC_r

Cuts at:    964    1032    2240    2414    2805    2832    4177     964 
   Size:         68    1208     174     391      27    1345    1442

  Fragments arranged by size: 

                1442    1345    1208     391     174      68      27
 
EagI C'GGCC_G

Cuts at:    964    2240     964 
   Size:       1276    3379
 
EarI CTCTTCn'nnn_

Cuts at:   1127    2678    2888    1127 
   Size:       1551     210    2894

  Fragments arranged by size: 

                2894    1551     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2863    3173    3319    4465    2863 
   Size:        310     146    1146    3053

  Fragments arranged by size: 

                3053    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1667    1667 
   Size:       4655
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    295     472    1264    1450    2480    2912    3659    3927     295 
   Size:        177     792     186    1030     432     747     268    1023

  Fragments arranged by size: 

                1030    1023     792     747     432     268     186     177
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    718     718 
   Size:       4655
 
EcoO109I rG'GnC_Cy

Cuts at:    150     192     702     972    1794     150 
   Size:         42     510     270     822    3011

  Fragments arranged by size: 

                3011     822     510     270      42
 
EcoRI G'AATT_C

Cuts at:   1659    1659 
   Size:       4655
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2674    2674 
   Size:       4655
 
FspI TGC'GCA

Cuts at:   2436    2436 
   Size:       4655
 
HaeII r_GCGC'y

Cuts at:    186    2089    2337    3359    4338     186 
   Size:       1903     248    1022     979     503

  Fragments arranged by size: 

                1903    1022     979     503     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    325     357     874     906    1099    1131    2497    2527    2529 
   Size:         32     517      32     193      32    1366      30       2

Cuts at:   2529    2559     325 
   Size:         30    2421

  Fragments arranged by size: 

                2421    1366     517     193      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1694    1694 
   Size:       4655
 
HindIII A'AGCT_T

Cuts at:   1234    1234 
   Size:       4655
 
KasI G'GCGC_C

Cuts at:   2085    2333    2085 
   Size:        248    4407
 
KpnI G_GTAC'C

Cuts at:   1675    1675 
   Size:       4655
 
MluI A'CGCG_T

Cuts at:   4088    4088 
   Size:       4655
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    450     458    3326    3510     450 
   Size:          8    2868     184    1595

  Fragments arranged by size: 

                2868    1595     184       8
 
MscI TGG'CCA

Cuts at:   2416    2416 
   Size:       4655
 
MslI CAynn'nnrTG

Cuts at:    950    1034    1459    2644    2771     950 
   Size:         84     425    1185     127    2834

  Fragments arranged by size: 

                2834    1185     425     127      84
 
MspA1I CmG'CkG

Cuts at:    213    1766    2080    2440    3453    3698     213 
   Size:       1553     314     360    1013     245    1170

  Fragments arranged by size: 

                1553    1170    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1321    2836    1321 
   Size:       1515    3140
 
NarI GG'CG_CC

Cuts at:   2086    2334    2086 
   Size:        248    4407
 
NcoI C'CATG_G

Cuts at:    107    1029     107 
   Size:        922    3733
 
NdeI CA'TA_TG

Cuts at:   1379    1379 
   Size:       4655
 
NgoMIV G'CCGG_C

Cuts at:   1319    2834    1319 
   Size:       1515    3140
 
NspI r_CATG'y

Cuts at:   1708    2208    3115    1708 
   Size:        500     907    3248

  Fragments arranged by size: 

                3248     907     500
 
PasI CC'CwG_GG

Cuts at:   1170    1170 
   Size:       4655
 
PciI A'CATG_T

Cuts at:   3111    3111 
   Size:       4655
 
PflMI CCAn_nnn'nTGG

Cuts at:   1170    1170 
   Size:       4655
 
PfoI T'CCnGG_A

Cuts at:    713    1427     713 
   Size:        714    3941
 
PmeI GTTT'AAAC

Cuts at:   1654    1654 
   Size:       4655
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    710     742    3794    3826     710 
   Size:         32    3052      32    1539

  Fragments arranged by size: 

                3052    1539      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    150     192     702     972     150 
   Size:         42     510     270    3833

  Fragments arranged by size: 

                3833     510     270      42
 
PsiI TTA'TAA

Cuts at:    851     851 
   Size:       4655
 
PstI C_TGCA'G

Cuts at:    384    1628    1702    2387     384 
   Size:       1244      74     685    2652

  Fragments arranged by size: 

                2652    1244     685      74
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4655
 
PvuII CAG'CTG

Cuts at:    213    2080    2440     213 
   Size:       1867     360    2428

  Fragments arranged by size: 

                2428    1867     360
 
RsrII CG'GwC_CG

Cuts at:   2850    2850 
   Size:       4655
 
SacI G_AGCT'C

Cuts at:   1669    1669 
   Size:       4655
 
SalI G'TCGA_C

Cuts at:   1692    1692 
   Size:       4655
 
SanDI GG'GwC_CC

Cuts at:    150     150 
   Size:       4655
 
SapI GCTCTTCn'nnn_

Cuts at:   2678    2888    2678 
   Size:        210    4445
 
SbfI CC_TGCA'GG

Cuts at:   1702    1702 
   Size:       4655
 
ScaI AGT'ACT

Cuts at:    610    4172     610 
   Size:       3562    1093
 
SexAI A'CCwGG_T

Cuts at:    623     623 
   Size:       4655
 
SfcI C'TryA_G

Cuts at:     33     380    1624    1698    2383    3376    3567      33 
   Size:        347    1244      74     685     993     191    1121

  Fragments arranged by size: 

                1244    1121     993     685     347     191      74
 
SfoI GGC'GCC

Cuts at:   2087    2335    2087 
   Size:        248    4407
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4655
 
SmaI CCC'GGG

Cuts at:    197    1677     197 
   Size:       1480    3175
 
SmlI C'TyrA_G

Cuts at:   1810    2725    3217    3479    1810 
   Size:        915     492     262    2986

  Fragments arranged by size: 

                2986     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67     201      67 
   Size:        134    4521
 
SphI G_CATG'C

Cuts at:   1708    2208    1708 
   Size:        500    4155
 
SspI AAT'ATT

Cuts at:   1197    1197 
   Size:       4655
 
StuI AGG'CCT

Cuts at:   1249    1249 
   Size:       4655
 
StyI C'CwwG_G

Cuts at:    107     749    1029    1789    2143     107 
   Size:        642     280     760     354    2619

  Fragments arranged by size: 

                2619     760     642     354     280
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2238    2371    2830    3039    2238 
   Size:        133     459     209    3854

  Fragments arranged by size: 

                3854     459     209     133
 
TatI w'GTAC_w

Cuts at:    592     608    1091    4170     592 
   Size:         16     483    3079    1077

  Fragments arranged by size: 

                3079    1077     483      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    570     602    4172    4204     570 
   Size:         32    3570      32    1021

  Fragments arranged by size: 

                3570    1021      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2452    2452 
   Size:       4655
 
XbaI T'CTAG_A

Cuts at:   1686    1686 
   Size:       4655
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1353    2150    1353 
   Size:        797    3858
 
XmaI C'CCGG_G

Cuts at:    195    1675     195 
   Size:       1480    3175
 
XmnI GAAnn'nnTTC

Cuts at:    184    1480     184 
   Size:       1296    3359
 
ZraI GAC'GTC

Cuts at:   1605    1605 
   Size:       4655
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI     PsiI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI    AflII     AleI     AloI     ApaI     AscI    AvrII     BaeI
     BbsI    BbvCI    BfrBI    BmgBI     BmtI    BsiWI     BsmI   BssHII
  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV     FseI    FspAI     HpaI
     MfeI     NheI     NotI     NruI     NsiI     PacI     PmlI    PshAI
   PspOMI    PspXI     PsrI    SacII     SfiI    SgrAI    SnaBI     SrfI
     SwaI     XhoI
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