Restriction map for pF1KB5454
 (Linear) (Six-base) MAPSORT of: KIBB5454.seq  Check: 1978  from: 1  to: 1542



KIBB5454.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AatII G_ACGT'C

Cuts at:      0    1499    1542 
   Size:       1499      43
 
AccI GT'mk_AC

Cuts at:      0     892    1542 
   Size:        892     650
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     364    1542 
   Size:        187     177    1178

  Fragments arranged by size: 

                1178     187     177
 
AfeI AGC'GCT

Cuts at:      0      76    1542 
   Size:         76    1466
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1005    1542 
   Size:       1005     537
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     842    1369    1542 
   Size:        842     527     173

  Fragments arranged by size: 

                 842     527     173
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1062    1542 
   Size:       1062     480
 
AvaI C'yCGr_G

Cuts at:      0      87    1542 
   Size:         87    1455
 
BanII G_rGCy'C

Cuts at:      0     919    1156    1542 
   Size:        919     237     386

  Fragments arranged by size: 

                 919     386     237
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     171     205    1542 
   Size:        171      34    1337

  Fragments arranged by size: 

                1337     171      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     562    1542 
   Size:        562     980
 
BlpI GC'TnA_GC

Cuts at:      0     718    1542 
   Size:        718     824
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     933    1194    1542 
   Size:        933     261     348

  Fragments arranged by size: 

                 933     348     261
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1156    1342    1542 
   Size:       1156     186     200

  Fragments arranged by size: 

                1156     200     186
 
Bpu10I CC'TnA_GC

Cuts at:      0    1155    1542 
   Size:       1155     387
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1182    1542 
   Size:       1182     360
 
BsaHI Gr'CG_yC

Cuts at:      0    1496    1542 
   Size:       1496      46
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1129    1159    1542 
   Size:       1129      30     383

  Fragments arranged by size: 

                1129     383      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     313    1060    1542 
   Size:        313     747     482

  Fragments arranged by size: 

                 747     482     313
 
BseYI C'CCAG_C

Cuts at:      0      45     565    1542 
   Size:         45     520     977

  Fragments arranged by size: 

                 977     520      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     820    1542 
   Size:        400     420     722

  Fragments arranged by size: 

                 722     420     400
 
BsiEI CG_ry'CG

Cuts at:      0     859    1542 
   Size:        859     683
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      55    1487    1542 
   Size:         55    1432      55

  Fragments arranged by size: 

                1432      55      55
 
Bsp1286I G_dGCh'C

Cuts at:      0     919    1156    1542 
   Size:        919     237     386

  Fragments arranged by size: 

                 919     386     237
 
BspHI T'CATG_A

Cuts at:      0     683    1542 
   Size:        683     859
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     875     904    1542 
   Size:        875      29     638

  Fragments arranged by size: 

                 875     638      29
 
BsrBI CCG'CTC

Cuts at:      0     360     381    1542 
   Size:        360      21    1161

  Fragments arranged by size: 

                1161     360      21
 
BsrDI GCAATG_nn'

Cuts at:      0     824    1542 
   Size:        824     718
 
BsrFI r'CCGG_y

Cuts at:      0    1211    1542 
   Size:       1211     331
 
BsrGI T'GTAC_A

Cuts at:      0     484    1542 
   Size:        484    1058
 
BstBI TT'CG_AA

Cuts at:      0    1218    1542 
   Size:       1218     324
 
BstEII G'GTnAC_C

Cuts at:      0    1530    1542 
   Size:       1530      12
 
BstYI r'GATC_y

Cuts at:      0       9    1542 
   Size:          9    1533
 
BtgI C'CryG_G

Cuts at:      0     557     921     936    1542 
   Size:        557     364      15     606

  Fragments arranged by size: 

                 606     557     364      15
 
BtsI GCAGTG_nn'

Cuts at:      0     768    1542 
   Size:        768     774
 
DraIII CAC_nnn'GTG

Cuts at:      0    1083    1542 
   Size:       1083     459
 
EaeI y'GGCC_r

Cuts at:      0     856     924    1542 
   Size:        856      68     618

  Fragments arranged by size: 

                 856     618      68
 
EagI C'GGCC_G

Cuts at:      0     856    1542 
   Size:        856     686
 
EarI CTCTTCn'nnn_

Cuts at:      0    1019    1542 
   Size:       1019     523
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     364    1156    1342    1542 
   Size:        187     177     792     186     200

  Fragments arranged by size: 

                 792     200     187     186     177
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     610    1542 
   Size:        610     932
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42      84     594     864    1542 
   Size:         42      42     510     270     678

  Fragments arranged by size: 

                 678     510     270      42      42
 
HaeII r_GCGC'y

Cuts at:      0      78    1542 
   Size:         78    1464
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     217     249     766     798     991    1023    1542 
   Size:        217      32     517      32     193      32     519

  Fragments arranged by size: 

                 519     517     217     193      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1126    1542 
   Size:       1126     416
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     342     350    1542 
   Size:        342       8    1192

  Fragments arranged by size: 

                1192     342       8
 
MslI CAynn'nnrTG

Cuts at:      0     842     926    1351    1542 
   Size:        842      84     425     191

  Fragments arranged by size: 

                 842     425     191      84
 
MspA1I CmG'CkG

Cuts at:      0     105    1542 
   Size:        105    1437
 
NaeI GCC'GGC

Cuts at:      0    1213    1542 
   Size:       1213     329
 
NcoI C'CATG_G

Cuts at:      0     921    1542 
   Size:        921     621
 
NdeI CA'TA_TG

Cuts at:      0    1271    1542 
   Size:       1271     271
 
NgoMIV G'CCGG_C

Cuts at:      0    1211    1542 
   Size:       1211     331
 
PasI CC'CwG_GG

Cuts at:      0    1062    1542 
   Size:       1062     480
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1062    1542 
   Size:       1062     480
 
PfoI T'CCnGG_A

Cuts at:      0     605    1319    1542 
   Size:        605     714     223

  Fragments arranged by size: 

                 714     605     223
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     602     634    1542 
   Size:        602      32     908

  Fragments arranged by size: 

                 908     602      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      42      84     594     864    1542 
   Size:         42      42     510     270     678

  Fragments arranged by size: 

                 678     510     270      42      42
 
PsiI TTA'TAA

Cuts at:      0     743    1542 
   Size:        743     799
 
PstI C_TGCA'G

Cuts at:      0     276    1520    1542 
   Size:        276    1244      22

  Fragments arranged by size: 

                1244     276      22
 
PvuII CAG'CTG

Cuts at:      0     105    1542 
   Size:        105    1437
 
SanDI GG'GwC_CC

Cuts at:      0      42    1542 
   Size:         42    1500
 
ScaI AGT'ACT

Cuts at:      0     502    1542 
   Size:        502    1040
 
SexAI A'CCwGG_T

Cuts at:      0     515    1542 
   Size:        515    1027
 
SfcI C'TryA_G

Cuts at:      0     272    1516    1542 
   Size:        272    1244      26

  Fragments arranged by size: 

                1244     272      26
 
SmaI CCC'GGG

Cuts at:      0      89    1542 
   Size:         89    1453
 
SpeI A'CTAG_T

Cuts at:      0      93    1542 
   Size:         93    1449
 
SspI AAT'ATT

Cuts at:      0    1089    1542 
   Size:       1089     453
 
StuI AGG'CCT

Cuts at:      0    1141    1542 
   Size:       1141     401
 
StyI C'CwwG_G

Cuts at:      0     641     921    1542 
   Size:        641     280     621

  Fragments arranged by size: 

                 641     621     280
 
TatI w'GTAC_w

Cuts at:      0     484     500     983    1542 
   Size:        484      16     483     559

  Fragments arranged by size: 

                 559     484     483      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     462     494    1542 
   Size:        462      32    1048

  Fragments arranged by size: 

                1048     462      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1245    1542 
   Size:       1245     297
 
XmaI C'CCGG_G

Cuts at:      0      87    1542 
   Size:         87    1455
 
XmnI GAAnn'nnTTC

Cuts at:      0      76    1372    1542 
   Size:         76    1296     170

  Fragments arranged by size: 

                1296     170      76
 
ZraI GAC'GTC

Cuts at:      0    1497    1542 
   Size:       1497      45
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI     AhdI     AlfI    AlwNI     AvaI
    BanII     BcgI     BglI     BlpI     BplI     BpmI   Bpu10I     BsaI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BstBI   BstEII
    BstYI     BtgI     BtsI   DraIII     EaeI     EagI     EarI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I  HindIII     MmeI     MslI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     PasI    PflMI     PfoI     PpiI
    PpuMI     PsiI     PstI    PvuII    SanDI     ScaI    SexAI     SfcI
     SmaI     SpeI     SspI     StuI     StyI     TatI     TstI     XcmI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII   AflIII     AgeI     AleI     AloI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI
    BglII Bme1580I    BmgBI     BmrI     BmtI    BpuEI    BsaAI    BsaBI
    BsaWI  BsiHKAI    BsiWI     BsmI    BspEI   BssHII    BssSI   BstAPI
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NarI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI
     PmlI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SphI     SrfI     SwaI    TaqII  Tth111I     XbaI
     XhoI
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