Restriction map for pF1KE1035
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaII       
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BclI        BfrI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaHI       BsaI        BseAI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       ClaI        CpoI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SapI        SbfI        SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       StuI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI          181  GTCGAC               GT^[AC][GT]AC
AfaI          792  GTAC                 GT^AC
Aor51HI       401  AGCGCT               AGC^GCT
Asp700I       637  GAAATTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          25  GAGCTC               G[AT]GC[AT]^C
AvaI          365  CTCGGG               C^[CT]CG[GA]G
BanI         1144  GGCACC               G^G[CT][GA]CC
BbvCI          79  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1049  GCATGGACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         524  GTATCC               GTATCC(6/5)
BcnI          171  CCGGG                CC^[GC]GG
BglI         1136  GCCGGTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         743  AGATCT               A^GATCT
BsaAI         796  TACGTG               [CT]AC^GT[GA]
BsaBI        1251  GATAGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         824  ACCGGA               [AT]^CCGG[AT]
BsiHKAI        25  GAGCTC               G[AT]GC[AT]^C
BsmBI         760  GAGACG               CGTCTC(1/5)
BsmFI         511  GGGAC                GGGAC(10/14)
BsoBI         365  CTCGGG               C^[CT]CG[GA]G
BspHI         671  TCATGA               T^CATGA
BsrBI        1060  GAGCGG               CCGCTC(-3/-3)
BsrFI        1136  GCCGGT               [GA]^CCGG[CT]
BssSI         658  CACGAG               CACGAG(-5/-1)
BstAPI        777  GCATGGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI          402  GCGC                 GCG^C
Cfr10I       1136  GCCGGT               [GA]^CCGG[CT]
DraI          997  TTTAAA               TTT^AAA
DraII          83  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI          954  CCGTGG               C^C[GA][CT]GG
EarI          472  GAAGAG               CTCTTC(1/4)
Eco47III      401  AGCGCT               AGC^GCT
EcoNI         255  CCTGTGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       83  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1016  GAATTC               G^AATTC
HaeII         401  AGCGCT               [GA]GCGC^[CT]
HhaI          402  GCGC                 GCG^C
HinP1I        402  GCGC                 G^CGC
Ksp632I       472  GAAGAG               CTCTTC(1/4)
MaeII         797  ACGT                 A^CGT
MamI         1251  GATAGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI         1164  CACATTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          171  CCGGG                CC^[GC]GG
PflMI         325  CCAGTTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          513  GACTC                GAGTC(4/5)
PshAI         616  GACATGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          671  TCATGA               T^CATGA
RsaI          792  GTAC                 GT^AC
SacI           25  GAGCTC               GAGCT^C
SalI          181  GTCGAC               G^TCGAC
ScaI          791  AGTACT               AGT^ACT
SexAI         863  ACCAGGT              A^CC[AT]GGT
SpeI          918  ACTAGT               A^CTAGT
SspI         1228  AATATT               AAT^ATT
TfiI         1099  GATTC                G^A[AT]TC
Van91I        325  CCAGTTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          637  GAAATTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcsI          638  AAATTT               [GA]^AATT[CT]
             1016  GAATTC              
AlwNI          59  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              326  CAGTTACTG           
AocI          229  CCTCAGG              CC^T[ACGT]AGG
             1093  CCTGAGG             
ApoI          638  AAATTT               [GA]^AATT[CT]
             1016  GAATTC              
BalI          302  TGGCCA               TGG^CCA
             1124  TGGCCA              
BanII          25  GAGCTC               G[GA]GC[CT]^C
              108  GGGCTC              
BfaI          529  CTAG                 C^TAG
              919  CTAG                
BmyI           25  GAGCTC               G[AGT]GC[ACT]^C
              108  GGGCTC              
BpmI          515  CTCCAG               CTGGAG(16/14)
              319  CTGGAG              
BseRI        1260  CTCCTC               GAGGAG(10/8)
              970  GAGGAG              
BsgI          889  GTGCAG               GTGCAG(16/14)
             1207  GTGCAG              
Bsp1286I       25  GAGCTC               G[AGT]GC[ACT]^C
              108  GGGCTC              
BstXI         126  CCACAGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1148  CCAAGGGCTTGG        
Bsu36I        229  CCTCAGG              CC^T[ACGT]AGG
             1093  CCTGAGG             
Cac8I         494  GCCCGC               GC[ACGT]^[ACGT]GC
             1132  GCAGGC              
Eco81I        229  CCTCAGG              CC^T[ACGT]AGG
             1093  CCTGAGG             
EcoT14I       905  CCTTGG               C^C[AT][AT]GG
             1148  CCAAGG              
FauI          495  CCCGC                CCCGC(4/6)
             1062  GCGGG               
HgaI          771  GACGC                GACGC(5/10)
              621  GCGTC               
HincII        181  GTCGAC               GT[CT]^[GA]AC
              768  GTTGAC              
HindII        181  GTCGAC               GT[CT]^[GA]AC
              768  GTTGAC              
HinfI         513  GACTC                G^A[ACGT]TC
             1099  GATTC               
MaeI          529  CTAG                 C^TAG
              919  CTAG                
MluNI         302  TGGCCA               TGG^CCA
             1124  TGGCCA              
MscI          302  TGGCCA               TGG^CCA
             1124  TGGCCA              
PvuII          62  CAGCTG               CAG^CTG
              316  CAGCTG              
StyI          905  CCTTGG               C^C[AT][AT]GG
             1148  CCAAGG              
Tsp45I        143  GTGAC                ^GT[GC]AC
              359  GTCAC               
XcmI          236  CCATTCTGGGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              266  CCAAGTCGGGCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BsaJI       
BsiYI       BslI        BsmAI       BspMI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        TaqI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XhoII       
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