Restriction map for pF1KB6614
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspI        AvrII       BamHI       
BbeI        BbrPI       BbvCI       BciVI       BclI        
BfaI        BfrI        BlnI        BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KspI        MaeI        MaeII       
MamI        MluI        MroI        MseI        MslI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        TaqI        TfiI        Tru9I       
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I        356  GGTACC               G^GTACC
AgeI         1203  ACCGGT               A^CCGGT
AhdI         1209  GACCACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          376  GGGCCC               GGGCC^C
Asp718I       356  GGTACC               G^GTACC
AspEI        1209  GACCACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         904  GAGCTC               G[AT]GC[AT]^C
AviII         569  TGCGCA               TGC^GCA
BbsI         1119  GAAGAC               GAAGAC(2/6)
BcgI          146  GCAGCACCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         896  AGATCT               A^GATCT
BlpI          951  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      951  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1119  GAAGAC               GAAGAC(2/6)
BsaWI        1203  ACCGGT               [AT]^CCGG[AT]
BsiHKAI       904  GAGCTC               G[AT]GC[AT]^C
BspMI        1275  GCAGGT               ACCTGC(4/8)
BssSI         664  CACGAG               CACGAG(-5/-1)
BstEII       1206  GGTGACC              G^GT[ACGT]ACC
BstPI        1206  GGTGACC              G^GT[ACGT]ACC
BstXI         693  CCAGGTGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         951  GCTCAGC              GC^T[ACGT]AGC
DsaI          446  CCGTGG               C^C[GA][CT]GG
Eam1105I     1209  GACCACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1237  GAAGAG               CTCTTC(1/4)
EcoO65I      1206  GGTGACC              G^GT[ACGT]ACC
EcoT14I       926  CCTTGG               C^C[AT][AT]GG
FspI          569  TGCGCA               TGC^GCA
HinfI        1185  GACTC                G^A[ACGT]TC
KpnI          356  GGTACC               GGTAC^C
Ksp632I      1237  GAAGAG               CTCTTC(1/4)
MfeI          410  CAATTG               C^AATTG
MunI          410  CAATTG               C^AATTG
PinAI        1203  ACCGGT               A^CCGGT
PleI         1185  GACTC                GAGTC(4/5)
SacI          904  GAGCTC               GAGCT^C
SbfI          116  CCTGCAGG             CCTGCA^GG
ScaI          398  AGTACT               AGT^ACT
SmaI          372  CCCGGG               CCC^GGG
SmlI          631  CTCAAG               C^T[CT][GA]AG
Sse8387I      116  CCTGCAGG             CCTGCA^GG
StuI          923  AGGCCT               AGG^CCT
StyI          926  CCTTGG               C^C[AT][AT]GG
Tsp509I       411  AATT                 ^AATT
XmaCI         372  CCCGGG               C^CCGGG
XmaI          372  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwNI         529  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1027  CAGGGGCTG           
AvaI          154  CTCGGG               C^[CT]CG[GA]G
              372  CCCGGG              
BalI          596  TGGCCA               TGG^CCA
              857  TGGCCA              
BanI          106  GGTGCC               G^G[CT][GA]CC
              356  GGTACC              
BcnI          372  CCCGG                CC^[GC]GG
              373  CCGGG               
BglI           56  GCCTGGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              484  GCCCGTCTGGC         
BsgI          877  GTGCAG               GTGCAG(16/14)
             1009  GTGCAG              
BsmAI         520  GAGAC                GTCTC(1/5)
              988  GAGAC               
BsoBI         154  CTCGGG               C^[CT]CG[GA]G
              372  CCCGGG              
BstAPI         77  GCACCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              528  GCAGGCCCTGC         
EaeI          596  TGGCCA               [CT]^GGCC[GA]
              857  TGGCCA              
HgaI          724  GACGC                GACGC(5/10)
             1122  GACGC               
MluNI         596  TGGCCA               TGG^CCA
              857  TGGCCA              
MscI          596  TGGCCA               TGG^CCA
              857  TGGCCA              
NaeI           86  GCCGGC               GCC^GGC
              109  GCCGGC              
NciI          372  CCCGG                CC^[GC]GG
              373  CCGGG               
NgoMIV         86  GCCGGC               G^CCGGC
              109  GCCGGC              
TspRI         327  CACTG                CA[GC]TG[ACGT][ACGT]^
              867  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaII       BanII       BbvI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
EcoNI       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MflI        MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TseI        
Tsp45I      XcmI        XhoII       
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