Restriction map for pF1KE0155
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tth111I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII         177  CGCG                 CG^CG
AcyI          388  GGCGTC               G[GA]^CG[CT]C
AfaI          714  GTAC                 GT^AC
AlwNI         417  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I        15  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         593  GGACC                G^G[AT]CC
BanI          664  GGTGCC               G^G[CT][GA]CC
BbvCI         528  CCTCAGC              CCTCAGC(-5/-2)
BcnI          552  CCGGG                CC^[GC]GG
BglI          646  GCCATGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         711  CACGTA               [CT]AC^GT[GA]
BsaHI         388  GGCGTC               G[GA]^CG[CT]C
BseRI         586  GAGGAG               GAGGAG(10/8)
BsgI          622  GTGCAG               GTGCAG(16/14)
BsmAI         173  GTCTC                GTCTC(1/5)
BsmI          319  GCATTC               GAATGC(1/-1)
BspLU11I      618  ACATGT               A^CATGT
BsrDI          29  CATTGC               GCAATG(2/0)
BsrI          402  ACTGG                ACTGG(1/-1)
BstUI         177  CGCG                 CG^CG
CfoI          114  GCGC                 GCG^C
DpnII         186  GATC                 ^GATC
DraII         601  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      601  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       647  CCATGG               C^C[AT][AT]GG
HaeII         113  AGCGCC               [GA]GCGC^[CT]
HapII         552  CCGG                 C^CGG
HhaI          114  GCGC                 GCG^C
Hin1I         388  GGCGTC               G[GA]^CG[CT]C
HinP1I        114  GCGC                 G^CGC
HpaII         552  CCGG                 C^CGG
HphI          471  GGTGA                GGTGA(8/7)
MaeIII        145  GTCAC                ^GT[ACGT]AC
MboI          186  GATC                 ^GATC
MspI          552  CCGG                 C^CGG
MvnI          177  CGCG                 CG^CG
NciI          552  CCGGG                CC^[GC]GG
NcoI          647  CCATGG               C^CATGG
NdeII         186  GATC                 ^GATC
PflMI         363  CCAGGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          684  GACTC                GAGTC(4/5)
PstI          414  CTGCAG               CTGCA^G
RsaI          714  GTAC                 GT^AC
Sau3AI        186  GATC                 ^GATC
SexAI         161  ACCTGGT              A^CC[AT]GGT
SfcI          414  CTGCAG               C^T[GA][CT]AG
StuI          284  AGGCCT               AGG^CCT
StyI          647  CCATGG               C^C[AT][AT]GG
TaqI           74  TCGA                 T^CGA
Tsp45I        145  GTCAC                ^GT[GC]AC
TthHB8I        74  TCGA                 T^CGA
Van91I        363  CCAGGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI           15  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AcsI           37  AAATTC               [GA]^AATT[CT]
              544  AAATTT              
AflIII        618  ACATGT               A^C[GA][CT]GT
              710  ACACGT              
AluI          301  AGCT                 AG^CT
              579  AGCT                
ApoI           37  AAATTC               [GA]^AATT[CT]
              544  AAATTT              
Cac8I         150  GCTGGC               GC[ACGT]^[ACGT]GC
              627  GCAGGC              
DsaI          368  CCGTGG               C^C[GA][CT]GG
              647  CCATGG              
EaeI          152  TGGCCG               [CT]^GGCC[GA]
              506  TGGCCG              
EarI          238  CTCTTC               CTCTTC(1/4)
              408  CTCTTC              
FauI           45  CCCGC                CCCGC(4/6)
              194  GCGGG               
HgaI          143  GCGTC                GACGC(5/10)
              389  GCGTC               
Ksp632I       238  CTCTTC               CTCTTC(1/4)
              408  CTCTTC              
MaeII         257  ACGT                 A^CGT
              712  ACGT                
MslI          486  CACTGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              498  CACAGTGATG          
MspA1I         46  CCGCTG               C[AC]G^C[GT]G
              417  CAGCGG              
NspI           96  GCATGT               [GA]CATG^[CT]
              618  ACATGT              
TfiI          612  GAATC                G^A[AT]TC
              672  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        BbvI        BsaJI       BsiYI       BslI        
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
ItaI        MboII       MnlI        MvaI        MwoI        
NlaIII      NlaIV       Sau96I      ScrFI       SfaNI       
TseI        Tsp509I     TspRI       
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