Restriction map for pF1KE0155
 (Linear) (Six-base) MAPSORT of: KIEE0155.seq  Check: 3778  from: 1  to: 729



KIEE0155.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     291     729 
   Size:         70     221     438

  Fragments arranged by size: 

                 438     221      70
 
AflIII A'CryG_T

Cuts at:      0     618     710     729 
   Size:        618      92      19

  Fragments arranged by size: 

                 618      92      19
 
AlwNI CAG_nnn'CTG

Cuts at:      0     422     729 
   Size:        422     307
 
ApoI r'AATT_y

Cuts at:      0      37     544     729 
   Size:         37     507     185

  Fragments arranged by size: 

                 507     185      37
 
BanI G'GyrC_C

Cuts at:      0     664     729 
   Size:        664      65
 
BbvCI CC'TCA_GC

Cuts at:      0     529     729 
   Size:        529     200
 
BglI GCCn_nnn'nGGC

Cuts at:      0     652     729 
   Size:        652      77
 
Bpu10I CC'TnA_GC

Cuts at:      0     529     581     729 
   Size:        529      52     148

  Fragments arranged by size: 

                 529     148      52
 
BsaAI yAC'GTr

Cuts at:      0     713     729 
   Size:        713      16
 
BsaHI Gr'CG_yC

Cuts at:      0     389     729 
   Size:        389     340
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     700     729 
   Size:        700      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     601     729 
   Size:        601     128
 
BseYI C'CCAG_C

Cuts at:      0      92     383     729 
   Size:         92     291     346

  Fragments arranged by size: 

                 346     291      92
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     643     729 
   Size:        643      86
 
BsmI GAATG_Cn'

Cuts at:      0     319     729 
   Size:        319     410
 
BsrDI GCAATG_nn'

Cuts at:      0      28     729 
   Size:         28     701
 
BtgI C'CryG_G

Cuts at:      0     368     647     729 
   Size:        368     279      82

  Fragments arranged by size: 

                 368     279      82
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     507     729 
   Size:        507     222
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     192     227     729 
   Size:        192      35     502

  Fragments arranged by size: 

                 502     192      35
 
EaeI y'GGCC_r

Cuts at:      0     152     506     729 
   Size:        152     354     223

  Fragments arranged by size: 

                 354     223     152
 
EarI CTCTTCn'nnn_

Cuts at:      0     244     414     729 
   Size:        244     170     315

  Fragments arranged by size: 

                 315     244     170
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     684     729 
   Size:        684      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     291     729 
   Size:         70     221     438

  Fragments arranged by size: 

                 438     221      70
 
EcoO109I rG'GnC_Cy

Cuts at:      0     602     729 
   Size:        602     127
 
HaeII r_GCGC'y

Cuts at:      0     117     729 
   Size:        117     612
 
MslI CAynn'nnrTG

Cuts at:      0     490     502     729 
   Size:        490      12     227

  Fragments arranged by size: 

                 490     227      12
 
MspA1I CmG'CkG

Cuts at:      0      48     419     729 
   Size:         48     371     310

  Fragments arranged by size: 

                 371     310      48
 
NcoI C'CATG_G

Cuts at:      0     647     729 
   Size:        647      82
 
NspI r_CATG'y

Cuts at:      0     100     622     729 
   Size:        100     522     107

  Fragments arranged by size: 

                 522     107     100
 
PasI CC'CwG_GG

Cuts at:      0     660     729 
   Size:        660      69
 
PciI A'CATG_T

Cuts at:      0     618     729 
   Size:        618     111
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     369     729 
   Size:        369     360
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477     729 
   Size:        445      32     252

  Fragments arranged by size: 

                 445     252      32
 
PstI C_TGCA'G

Cuts at:      0     418     729 
   Size:        418     311
 
SexAI A'CCwGG_T

Cuts at:      0     161     729 
   Size:        161     568
 
SfcI C'TryA_G

Cuts at:      0     414     729 
   Size:        414     315
 
StuI AGG'CCT

Cuts at:      0     286     729 
   Size:        286     443
 
StyI C'CwwG_G

Cuts at:      0     647     729 
   Size:        647      82
 
XmnI GAAnn'nnTTC

Cuts at:      0      19     729 
   Size:         19     710
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     BanI    BbvCI     BglI   Bpu10I
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI     BsmI    BsrDI
     BtgI    BtgZI    CspCI     EaeI     EarI     EciI  Eco57MI EcoO109I
    HaeII     MslI   MspA1I     NcoI     NspI     PasI     PciI    PflMI
     PpiI     PstI    SexAI     SfcI     StuI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaWI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI     DraI   DraIII
     DrdI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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