Restriction map for pF1KE0034
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# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BlnI        BsaAI       BsaHI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BspDI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BssHII      BssSI       BstBI       BstEII      BstPI       
BstUI       Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        KasI        
KpnI        KspI        MaeII       MluI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspI        
NspV        PacI        PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SwaI        
Tth111I     XcmI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccIII        316  TCCGGA               T^CCGGA
AvaII         522  GGACC                G^G[AT]CC
BanII        1321  GAGCCC               G[GA]GC[CT]^C
BbvI          735  GCTGC                GCAGC(8/12)
BglI          468  GCCTGACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         354  AGATCT               A^GATCT
BmyI         1321  GAGCCC               G[AGT]GC[ACT]^C
BsaBI         862  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI        1456  CCTTGG               C^C[ACGT][ACGT]GG
BsaWI         316  TCCGGA               [AT]^CCGG[AT]
BseAI         316  TCCGGA               T^CCGGA
BsgI          830  GTGCAG               GTGCAG(16/14)
BsmFI         488  GGGAC                GGGAC(10/14)
Bsp1286I     1321  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      796  TGTACA               T^GTACA
BspEI         316  TCCGGA               T^CCGGA
BsrGI         796  TGTACA               T^GTACA
Bst1107I     1337  GTATAC               GTA^TAC
BstAPI         94  GCAGTCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         720  CCACCGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         354  AGATCT               [GA]^GATC[CT]
BstZ17I      1337  GTATAC               GTA^TAC
DraI          651  TTTAAA               TTT^AAA
DraII         521  AGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII       1169  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I      521  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRV         688  GATATC               GAT^ATC
EcoT14I      1456  CCTTGG               C^C[AT][AT]GG
EcoT22I       433  ATGCAT               ATGCA^T
HapII         317  CCGG                 C^CGG
HinfI         515  GATTC                G^A[ACGT]TC
HpaII         317  CCGG                 C^CGG
MaeIII       1118  GTGAC                ^GT[ACGT]AC
MamI          862  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          673  CAATTG               C^AATTG
MflI          354  AGATCT               [GA]^GATC[CT]
MroI          316  TCCGGA               T^CCGGA
MslI          721  CACCGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          317  CCGG                 C^CGG
MunI          673  CAATTG               C^AATTG
NsiI          433  ATGCAT               ATGCA^T
PaeR7I        549  CTCGAG               C^TCGAG
PflMI         479  CCATCAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         521  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          390  CTGCAG               CTGCA^G
PvuII         733  CAGCTG               CAG^CTG
ScaI          310  AGTACT               AGT^ACT
SfcI          390  CTGCAG               C^T[GA][CT]AG
SspBI         796  TGTACA               T^GTACA
StuI          109  AGGCCT               AGG^CCT
StyI         1456  CCTTGG               C^C[AT][AT]GG
TfiI          515  GATTC                G^A[AT]TC
TseI          735  GCTGC                G^C[AT]GC
Tsp45I       1118  GTGAC                ^GT[GC]AC
Van91I        479  CCATCAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          549  CTCGAG               C^TCGAG
XhoII         354  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          199  GTCTAC               GT^[AC][GT]AC
             1337  GTATAC              
AciI          723  CCGC                 CCGC(-3/-1)
              750  CCGC                
AlwI         1107  GATCC                GGATC(4/5)
             1177  GATCC               
AvaI          549  CTCGAG               C^[CT]CG[GA]G
             1147  CTCGGG              
BalI          294  TGGCCA               TGG^CCA
              729  TGGCCA              
BbsI          532  GAAGAC               GAAGAC(2/6)
              940  GAAGAC              
BpmI          214  CTCCAG               CTGGAG(16/14)
             1189  CTCCAG              
BpuAI         532  GAAGAC               GAAGAC(2/6)
              940  GAAGAC              
BsaI           15  GAGACC               GGTCTC(1/5)
             1442  GAGACC              
BsiYI         155  CCGTAGAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              479  CCATCAAATGG         
BslI          155  CCGTAGAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              479  CCATCAAATGG         
BsoBI         549  CTCGAG               C^[CT]CG[GA]G
             1147  CTCGGG              
EaeI          294  TGGCCA               [CT]^GGCC[GA]
              729  TGGCCA              
EarI         1028  CTCTTC               CTCTTC(1/4)
             1246  GAAGAG              
Fnu4HI        735  GCTGC                GC^[ACGT]GC
              749  GCCGC               
HindIII       417  AAGCTT               A^AGCTT
             1134  AAGCTT              
ItaI          735  GCTGC                GC^[ACGT]GC
              749  GCCGC               
Ksp632I      1028  CTCTTC               CTCTTC(1/4)
             1246  GAAGAG              
MluNI         294  TGGCCA               TGG^CCA
              729  TGGCCA              
MscI          294  TGGCCA               TGG^CCA
              729  TGGCCA              
MspA1I        723  CCGCTG               C[AC]G^C[GT]G
              733  CAGCTG              
NlaIV         300  GGAACC               GG[ACGT]^[ACGT]CC
              522  GGACCC              
SmlI          105  CTTGAG               C^T[CT][GA]AG
              549  CTCGAG              
SspI         1019  AATATT               AAT^ATT
             1159  AATATT              
XbaI           36  TCTAGA               T^CTAGA
             1368  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BfaI        
BlpI        Bpu1102I    BseRI       BsmAI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CelII       
Cfr13I      DdeI        DpnII       EcoRII      FokI        
HaeIII      HphI        MaeI        MboI        MboII       
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        Tru9I       Tsp509I     TspRI       
TthHB8I     
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