Restriction map for pF1KE0034
 (Linear) (Six-base) MAPSORT of: KIEE0034.seq  Check: 5958  from: 1  to: 1488



KIEE0034.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AccI GT'mk_AC

Cuts at:      0     200    1338    1488 
   Size:        200    1138     150

  Fragments arranged by size: 

                1138     200     150
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     901    1488 
   Size:        110     791     587

  Fragments arranged by size: 

                 791     587     110
 
AleI CACnn'nnGTG

Cuts at:      0     725    1488 
   Size:        725     763
 
ApoI r'AATT_y

Cuts at:      0     177     334     765    1294    1488 
   Size:        177     157     431     529     194

  Fragments arranged by size: 

                 529     431     194     177     157
 
AvaI C'yCGr_G

Cuts at:      0     549    1147    1488 
   Size:        549     598     341

  Fragments arranged by size: 

                 598     549     341
 
BanII G_rGCy'C

Cuts at:      0    1325    1488 
   Size:       1325     163
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     539     947    1488 
   Size:        539     408     541

  Fragments arranged by size: 

                 541     539     408
 
BfrBI ATG'CAT

Cuts at:      0     435    1488 
   Size:        435    1053
 
BglI GCCn_nnn'nGGC

Cuts at:      0     474    1488 
   Size:        474    1014
 
BglII A'GATC_T

Cuts at:      0     354    1488 
   Size:        354    1134
 
BlpI GC'TnA_GC

Cuts at:      0     361     753    1214    1488 
   Size:        361     392     461     274

  Fragments arranged by size: 

                 461     392     361     274
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199    1174    1488 
   Size:        199     975     314

  Fragments arranged by size: 

                 975     314     199
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     126    1488 
   Size:        126    1362
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       9    1436    1488 
   Size:          9    1427      52

  Fragments arranged by size: 

                1427      52       9
 
BsaBI GATnn'nnATC

Cuts at:      0     866    1488 
   Size:        866     622
 
BsaWI w'CCGG_w

Cuts at:      0     316    1488 
   Size:        316    1172
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      18      48    1488 
   Size:         18      30    1440

  Fragments arranged by size: 

                1440      30      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      39     717     936    1488 
   Size:         39     678     219     552

  Fragments arranged by size: 

                 678     552     219      39
 
BseYI C'CCAG_C

Cuts at:      0     440     707    1324    1488 
   Size:        440     267     617     164

  Fragments arranged by size: 

                 617     440     267     164
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     851    1488 
   Size:        851     637
 
Bsp1286I G_dGCh'C

Cuts at:      0    1325    1488 
   Size:       1325     163
 
BspEI T'CCGG_A

Cuts at:      0     316    1488 
   Size:        316    1172
 
BsrGI T'GTAC_A

Cuts at:      0     796    1488 
   Size:        796     692
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     100    1488 
   Size:        100    1388
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     727    1488 
   Size:        727     761
 
BstYI r'GATC_y

Cuts at:      0     354    1488 
   Size:        354    1134
 
BstZ17I GTA'TAC

Cuts at:      0    1339    1488 
   Size:       1339     149
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     699    1488 
   Size:        699     789
 
BtsI GCAGTG_nn'

Cuts at:      0     839    1378    1488 
   Size:        839     539     110

  Fragments arranged by size: 

                 839     539     110
 
DraI TTT'AAA

Cuts at:      0     653    1488 
   Size:        653     835
 
DraIII CAC_nnn'GTG

Cuts at:      0    1174    1488 
   Size:       1174     314
 
EaeI y'GGCC_r

Cuts at:      0     294     729    1488 
   Size:        294     435     759

  Fragments arranged by size: 

                 759     435     294
 
EarI CTCTTCn'nnn_

Cuts at:      0    1034    1241    1488 
   Size:       1034     207     247

  Fragments arranged by size: 

                1034     247     207
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     199     901    1174    1488 
   Size:        110      89     702     273     314

  Fragments arranged by size: 

                 702     314     273     110      89
 
EcoO109I rG'GnC_Cy

Cuts at:      0     522    1488 
   Size:        522     966
 
EcoRV GAT'ATC

Cuts at:      0     690    1488 
   Size:        690     798
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      18      50     649     681    1488 
   Size:         18      32     599      32     807

  Fragments arranged by size: 

                 807     599      32      32      18
 
HindIII A'AGCT_T

Cuts at:      0     417    1134    1488 
   Size:        417     717     354

  Fragments arranged by size: 

                 717     417     354
 
MfeI C'AATT_G

Cuts at:      0     673    1488 
   Size:        673     815
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1189    1488 
   Size:       1189     299
 
MscI TGG'CCA

Cuts at:      0     296     731    1488 
   Size:        296     435     757

  Fragments arranged by size: 

                 757     435     296
 
MslI CAynn'nnrTG

Cuts at:      0     725    1488 
   Size:        725     763
 
MspA1I CmG'CkG

Cuts at:      0     725     735    1488 
   Size:        725      10     753

  Fragments arranged by size: 

                 753     725      10
 
NsiI A_TGCA'T

Cuts at:      0     437    1488 
   Size:        437    1051
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     485    1488 
   Size:        485    1003
 
PpuMI rG'GwC_Cy

Cuts at:      0     522    1488 
   Size:        522     966
 
PsiI TTA'TAA

Cuts at:      0    1046    1488 
   Size:       1046     442
 
PstI C_TGCA'G

Cuts at:      0     394    1488 
   Size:        394    1094
 
PvuII CAG'CTG

Cuts at:      0     735    1488 
   Size:        735     753
 
ScaI AGT'ACT

Cuts at:      0     312    1488 
   Size:        312    1176
 
SfcI C'TryA_G

Cuts at:      0     390    1488 
   Size:        390    1098
 
SmlI C'TyrA_G

Cuts at:      0     105     549    1488 
   Size:        105     444     939

  Fragments arranged by size: 

                 939     444     105
 
SspI AAT'ATT

Cuts at:      0    1021    1161    1488 
   Size:       1021     140     327

  Fragments arranged by size: 

                1021     327     140
 
StuI AGG'CCT

Cuts at:      0     111    1488 
   Size:        111    1377
 
StyI C'CwwG_G

Cuts at:      0    1456    1488 
   Size:       1456      32
 
TatI w'GTAC_w

Cuts at:      0     126     310     796    1488 
   Size:        126     184     486     692

  Fragments arranged by size: 

                 692     486     184     126
 
XbaI T'CTAG_A

Cuts at:      0      36    1368    1488 
   Size:         36    1332     120

  Fragments arranged by size: 

                1332     120      36
 
XhoI C'TCGA_G

Cuts at:      0     549    1488 
   Size:        549     939
 

 Enzymes that do cut:

     AccI     AcuI     AleI     ApoI     AvaI    BanII     BbsI    BfrBI
     BglI    BglII     BlpI     BpmI    BpuEI     BsaI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI Bsp1286I    BspEI    BsrGI   BstAPI
    BstXI    BstYI  BstZ17I    BtgZI     BtsI     DraI   DraIII     EaeI
     EarI  Eco57MI EcoO109I    EcoRV    Hin4I  HindIII     MfeI     MmeI
     MscI     MslI   MspA1I     NsiI    PflMI    PpuMI     PsiI     PstI
    PvuII     ScaI     SfcI     SmlI     SspI     StuI     StyI     TatI
     XbaI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BsaAI    BsaHI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII
   Bsu36I     BtgI     ClaI    CspCI     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI    TaqII     TstI  Tth111I     XcmI     XmaI
     XmnI     ZraI
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