Restriction map for pF1KE0158
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BclI        BfaI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FauI        FbaI        FseI        HaeII       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MaeII       MfeI        MluI        
MluNI       MroI        MscI        MslI        MunI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Van91I      XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII         248  CGCG                 CG^CG
AcyI          117  GGCGTC               G[GA]^CG[CT]C
AocI          825  CCTAAGG              CC^T[ACGT]AGG
AspI          685  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         413  TGCGCA               TGC^GCA
BamHI         523  GGATCC               G^GATCC
BbsI          796  GAAGAC               GAAGAC(2/6)
BciVI         731  GTATCC               GTATCC(6/5)
BglII         768  AGATCT               A^GATCT
BpmI          482  CTCCAG               CTGGAG(16/14)
BpuAI         796  GAAGAC               GAAGAC(2/6)
BsaBI         726  GATGGGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         117  GGCGTC               G[GA]^CG[CT]C
BsaI          127  GAGACC               GGTCTC(1/5)
BsaWI         776  ACCGGA               [AT]^CCGG[AT]
BsmBI         690  CGTCTC               CGTCTC(1/5)
BspMI         317  ACCTGC               ACCTGC(4/8)
BsrDI         299  GCAATG               GCAATG(2/0)
BsrI          172  CCAGT                ACTGG(1/-1)
BstAPI        439  GCAGCTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         248  CGCG                 CG^CG
Bsu36I        825  CCTAAGG              CC^T[ACGT]AGG
Eco81I        825  CCTAAGG              CC^T[ACGT]AGG
EcoRV         856  GATATC               GAT^ATC
EcoT14I       701  CCAAGG               C^C[AT][AT]GG
FspI          413  TGCGCA               TGC^GCA
Hin1I         117  GGCGTC               G[GA]^CG[CT]C
HinfI         762  GAGTC                G^A[ACGT]TC
MamI          726  GATGGGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          463  TTAA                 T^TAA
MspA1I        440  CAGCTG               C[AC]G^C[GT]G
MvnI          248  CGCG                 CG^CG
NdeI          224  CATATG               CA^TATG
PflFI         685  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          762  GAGTC                GAGTC(4/5)
PvuII         440  CAGCTG               CAG^CTG
SfaNI         445  GATGC                GCATC(5/9)
StyI          701  CCAAGG               C^C[AT][AT]GG
TaqI           63  TCGA                 T^CGA
Tru9I         463  TTAA                 T^TAA
Tsp509I        81  AATT                 ^AATT
Tth111I       685  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I        63  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI          378  CCGC                 CCGC(-3/-1)
              208  GCGG                
AlwI          524  GATCC                GGATC(4/5)
              523  GGATC               
AvaI          113  CCCGGG               C^[CT]CG[GA]G
              230  CCCGGG              
BmyI          110  GTGCCC               G[AGT]GC[ACT]^C
              166  GTGCCC              
BsoBI         113  CCCGGG               C^[CT]CG[GA]G
              230  CCCGGG              
Bsp1286I      110  GTGCCC               G[AGT]GC[ACT]^C
              166  GTGCCC              
BstYI         523  GGATCC               [GA]^GATC[CT]
              768  AGATCT              
DpnII         524  GATC                 ^GATC
              769  GATC                
DraII         587  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              821  AGGTCCT             
EcoO109I      587  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              821  AGGTCCT             
HgaI          118  GCGTC                GACGC(5/10)
              689  GCGTC               
HphI          566  TCACC                GGTGA(8/7)
              747  TCACC               
MaeIII         22  GTGAC                ^GT[ACGT]AC
              560  GTGAC               
MboI          524  GATC                 ^GATC
              769  GATC                
MflI          523  GGATCC               [GA]^GATC[CT]
              768  AGATCT              
NdeII         524  GATC                 ^GATC
              769  GATC                
PpuMI         587  AGGTCCC              [GA]G^G[AT]CC[CT]
              821  AGGTCCT             
PstI          391  CTGCAG               CTGCA^G
              663  CTGCAG              
Sau3AI        524  GATC                 ^GATC
              769  GATC                
SfcI          391  CTGCAG               C^T[GA][CT]AG
              663  CTGCAG              
SmaI          113  CCCGGG               CCC^GGG
              230  CCCGGG              
Tsp45I         22  GTGAC                ^GT[GC]AC
              560  GTGAC               
TspRI          18  CACTG                CA[GC]TG[ACGT][ACGT]^
              816  CACTG               
XhoII         523  GGATCC               [GA]^GATC[CT]
              768  AGATCT              
XmaCI         113  CCCGGG               C^CCGGG
              230  CCCGGG              
XmaI          113  CCCGGG               C^CCGGG
              230  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AluI        AvaII       BbvI        BcnI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        Ksp632I     MboII       MnlI        
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       Sau96I      ScrFI       TseI        
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