Restriction map for pF1KE0158
 (Linear) (Six-base) MAPSORT of: KIEE0158.seq  Check: 8544  from: 1  to: 876



KIEE0158.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     695     757     876 
   Size:        370     325      62     119

  Fragments arranged by size: 

                 370     325     119      62
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     317     876 
   Size:        317     559
 
AvaI C'yCGr_G

Cuts at:      0     113     230     876 
   Size:        113     117     646

  Fragments arranged by size: 

                 646     117     113
 
BamHI G'GATC_C

Cuts at:      0     523     876 
   Size:        523     353
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     803     876 
   Size:        803      73
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     742     876 
   Size:        742     134
 
BglII A'GATC_T

Cuts at:      0     768     876 
   Size:        768     108
 
Bme1580I G_kGCm'C

Cuts at:      0     114     170     876 
   Size:        114      56     706

  Fragments arranged by size: 

                 706     114      56
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     166     876 
   Size:        166     710
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467     876 
   Size:        467     409
 
Bpu10I CC'TnA_GC

Cuts at:      0     373     593     876 
   Size:        373     220     283

  Fragments arranged by size: 

                 373     283     220
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     121     876 
   Size:        121     755
 
BsaBI GATnn'nnATC

Cuts at:      0     730     876 
   Size:        730     146
 
BsaHI Gr'CG_yC

Cuts at:      0     118     876 
   Size:        118     758
 
BsaWI w'CCGG_w

Cuts at:      0     776     876 
   Size:        776     100
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     232     346     586     876 
   Size:        232     114     240     290

  Fragments arranged by size: 

                 290     240     232     114
 
BseYI C'CCAG_C

Cuts at:      0     274     402     876 
   Size:        274     128     474

  Fragments arranged by size: 

                 474     274     128
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     696     876 
   Size:        696     180
 
Bsp1286I G_dGCh'C

Cuts at:      0     114     170     876 
   Size:        114      56     706

  Fragments arranged by size: 

                 706     114      56
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     326     876 
   Size:        326     550
 
BsrDI GCAATG_nn'

Cuts at:      0     306     876 
   Size:        306     570
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     445     876 
   Size:        445     431
 
BstYI r'GATC_y

Cuts at:      0     523     768     876 
   Size:        523     245     108

  Fragments arranged by size: 

                 523     245     108
 
Bsu36I CC'TnA_GG

Cuts at:      0     826     876 
   Size:        826      50
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     177     876 
   Size:        177     699
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     303     338     416     451     876 
   Size:        303      35      78      35     425

  Fragments arranged by size: 

                 425     303      78      35      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     209     507     671     876 
   Size:        209     298     164     205

  Fragments arranged by size: 

                 298     209     205     164
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     467     695     757     876 
   Size:        370      97     228      62     119

  Fragments arranged by size: 

                 370     228     119      97      62
 
EcoO109I rG'GnC_Cy

Cuts at:      0     588     822     876 
   Size:        588     234      54

  Fragments arranged by size: 

                 588     234      54
 
EcoRV GAT'ATC

Cuts at:      0     858     876 
   Size:        858      18
 
FspI TGC'GCA

Cuts at:      0     415     876 
   Size:        415     461
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     463     495     586     618     676     708     753     785 
   Size:        463      32      91      32      58      32      45      32

Cuts at:    785     876 
   Size:         91

  Fragments arranged by size: 

                 463      91      91      58      45      32      32      32
                  32
 
MspA1I CmG'CkG

Cuts at:      0     442     876 
   Size:        442     434
 
NdeI CA'TA_TG

Cuts at:      0     225     876 
   Size:        225     651
 
PfoI T'CCnGG_A

Cuts at:      0     483     876 
   Size:        483     393
 
PpuMI rG'GwC_Cy

Cuts at:      0     588     822     876 
   Size:        588     234      54

  Fragments arranged by size: 

                 588     234      54
 
PstI C_TGCA'G

Cuts at:      0     395     667     876 
   Size:        395     272     209

  Fragments arranged by size: 

                 395     272     209
 
PvuII CAG'CTG

Cuts at:      0     442     876 
   Size:        442     434
 
SfcI C'TryA_G

Cuts at:      0     391     663     876 
   Size:        391     272     213

  Fragments arranged by size: 

                 391     272     213
 
SmaI CCC'GGG

Cuts at:      0     115     232     876 
   Size:        115     117     644

  Fragments arranged by size: 

                 644     117     115
 
SrfI GCCC'GGGC

Cuts at:      0     115     876 
   Size:        115     761
 
StyI C'CwwG_G

Cuts at:      0     701     876 
   Size:        701     175
 
Tth111I GACn'n_nGTC

Cuts at:      0     688     876 
   Size:        688     188
 
XmaI C'CCGG_G

Cuts at:      0     113     230     876 
   Size:        113     117     646

  Fragments arranged by size: 

                 646     117     113
 

 Enzymes that do cut:

     AcuI     AlfI     AvaI    BamHI     BbsI    BciVI    BglII Bme1580I
     BmrI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI    BsmBI Bsp1286I    BspMI    BsrDI   BstAPI    BstYI   Bsu36I
    BtgZI    CspCI     EarI  Eco57MI EcoO109I    EcoRV     FspI    Hin4I
   MspA1I     NdeI     PfoI    PpuMI     PstI    PvuII     SfcI     SmaI
     SrfI     StyI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI
     BplI    BpuEI    BsaAI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I     BtgI     BtsI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FseI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     SwaI
    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmnI     ZraI
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